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Mus musculus

ABSTRACT: Epigenetic profiling of aorta macrophages of mice exposed to intermittent hypoxia (CD11ME)

PROVIDER: PRJNA342773 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4240525.fastq.gz	Fastqsanger.gz
	SRR4240526.fastq.gz	Fastqsanger.gz
	SRR4240527.fastq.gz	Fastqsanger.gz
	SRR4240528.fastq.gz	Fastqsanger.gz

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Project description:We hypothesize that the culture media collected from macrophages exposed to intermittent hypoxia will induce a greater pro-inflammatory gene profile in naïve cultured macrophages than will culture media collected from macrophages exposed to sustained hypoxia. We will evaluate gene expression using microarray analysis of RNA collected from RAW 264.7 macrophages cultured for 24 hours in DMEM media obtained from 1) cells cultured with intermittent hypoxia (2 minute cycles: 90 seconds at 40 Torr and 30 seconds at 8 Torr), 2) media exposed to intermittent hypoxia, 3) cells cultured with sustained hypoxia (8 Torr), 4) media exposed to sustained hypoxia and 4) standard tissue culture conditions (fresh DMEM media; reference).

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Project description:We hypothesize that cultured macrophages directly exposed intermittent hypoxia will have a greater change in expression in genes related to inflammatory response than macrophages exposed to sustained hypoxia. We will evaluate gene expression using microarray analysis of RNA collected from RAW 264.7 macrophages cultured under the following environmental conditions: 1) 4 hours of intermittent hypoxia (2 minute cycles: 90 seconds at 40 Torr and 30 seconds at 8 Torr), 2) 4 hours of sustained hypoxia (8 Torr), and 3 ) standard tissue culture conditions (141 Torr; reference).

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GNPS_2017 Mouse intermittent hypoxia (IH) - Aorta & pulmonary artery

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Project description:ChIP-seq targeting H3K9ac and H3K27me3 histone modifications was carried out on macrophages isolated from aortas of mice exposed to intermittent hypoxia or room air conditions.

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Bidirectional Introgression between Mus musculus domesticus and Mus spretus.

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Project description:We exposed p14 mice to 56 day of forced intermittent hypoxia as a proxy stimlulus for pediatric OSA. After this exposure, we performed sn-RNA seq of the hippocampus of both normoxic controls and IH exposed mice to study potentially DEGs.

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