Project description:Transcriptional profiling of human hepatoellular carcinoma (HCC) cell line: MHCC-97H comparing control untreated MHCC-97H cells with MHCC-97H cells overexpressed ROCK1 protein. Goal was to determine the effect of ROCK1 on global MHCC-97H cell mRNA and lncRNA expression. Transcriptional profiling of human hepatoellular carcinoma (HCC) cell line: MHCC-97H comparing control untreated MHCC-97H cells with MHCC-97H cells overexpressed ROCK2 protein. Goal was to determine the effect of ROCK2 on global MHCC-97H cell mRNA and lncRNA expression. Transcriptional profiling of human hepatoellular carcinoma (HCC) cell line: MHCC-97H comparing MHCC-97H cells overexpressed ROCK1 with MHCC-97H cells overexpressed ROCK2 protein. Goal was to determine the distinction of ROCK1 & 2 on global MHCC-97H cell mRNA and lncRNA expression.
Project description:Whole-genome gene expression profiling of MHCC-97H cells, to further dissect the possible molecular mechanisms mediated by UGT2B15 downregulation.
Project description:Overexpression of PHGDH in MHCC-97H cells, enrichment of PHGDH-bound RNA by antibody, and identification of target genes using high-throughput sequencing
Project description:To investigate the function of CENPU in the regulation of hepatocellular carcinoma tumorigenesis , we established Huh-7 and MHCC-97H cell lines in which CENPU gene has been knocked down by siRNA. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells.
Project description:To study the mRNA modulating network and different expressed mRNA lists in MHCC-97H-SH-UCK2 cells, we use high-throughout method to genomically detect the expression profile of mRNA
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
Project description:TGM2 IP-MS was performed in MHCC-97H cells to screen the interactors of TGM2. 5-PT pulldown-MS was perfromed in MHCC-97H cells to identify proteins modified by serotonylation.
Project description:The highly metastatic MHCC-97H HCC cell line was orthotopic transplanted into the liver of nude mice to induce orthotopic tumor formation, and lung metastasis was observed. This experimental model not only reproduces the metastasis process of HCC in vivo, but also provides an important biological material for studying the tumor microenvironment and metastasis mechanism. Subsequently, we collected liver cancer tissues, and further conducted in-depth proteomic analysis of the collected liver tumor tissue samples, in order to reveal the key protein expression changes and potential signaling pathway regulation mechanisms during tumor development and metastasis, and lay the foundation for subsequent targeted therapy research.
