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Chromatin binding profiles of the APC tumor suppressor and changes in gene expression following APC silencing

ABSTRACT: Chromatin binding profiles of the APC tumor suppressor and changes in gene expression following APC silencing

PROVIDER: PRJNA387736 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5595740.fastq.gz	Fastqsanger.gz
	SRR5595734.fastq.gz	Fastqsanger.gz
	SRR5595735.fastq.gz	Fastqsanger.gz
	SRR5595736.fastq.gz	Fastqsanger.gz
	SRR5595737.fastq.gz	Fastqsanger.gz

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Differential gene expression upon shRNA-mediated silencing of APC in HT-29 colorectal cancer cells

Project description:Wnt signaling plays a pivotal role in colorectal cancer. Intrinsic activation of Wnt by mutational events, such as mutations in the tumor suppressor gene APC, represents the most frequent initiating event in this disease background. Long truncated versions of APC retain partial functionality, which leads to a sub-maximal, “just right” activation state of Wnt signaling supposed to be beneficial for disease initiation. In order to study the transcriptomic alterations of an over-stimulated Wnt signaling pathway, conditional shRNA-mediated silencing of APC was performed in chromosomal instable HT-29 CRC cells which express a 1555 amino acid variant of APC protein able to bind and partially inactivate β-catenin. To achieve this, cells were stably transduced with lentiviral particles encoding for a doxycyline-inducible shRNA directed against APC, or, as a control, a non-silencing shRNA (pTRIPZ inducible shRNA vectors, RHS4740-EG324, Horizon Dharmacon, CO, USA). 72 hours after APC silencing, total RNA was isolated and quality controlled for subsequent RNA-Seq Analysis (DKFZ Heidelberg, Genomic and Proteomic Core Facility) on a HiSeq 2000 instrument (Illumina). Overall, we observed bona-fide Wnt target genes, such as NKD1, AXIN2, PTK7, ASCL2, and SMOC2, and additional putative direct or indirect targets of Wnt signaling up-regulated upon shRNA-mediated APC silencing.

2020-06-25 | GSE131575 | GEO

Chromatin binding profiles of the APC tumor suppressor and changes in gene expression following APC silencing

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-05-24 | GSE99264 | GEO

The impact of P53 loss on transcriptome changes following loss of Apc in the intestine

Project description:BACKGROUND: p53 is an important tumor suppressor with a known role in the later stages of colorectal cancer, but its relevance to the early stages of neoplastic initiation remains somewhat unclear. Although p53-dependent regulation of Wnt signalling activity is known to occur, the importance of these regulatory mechanisms during the early stages of intestinal neoplasia has not been demonstrated. METHODS: We have conditionally deleted the Adenomatous Polyposis coli gene (Apc) from the adult murine intestine in wild type and p53 deficient environments and subsequently compared the phenotype and transcriptome profiles in both genotypes. RESULTS: Expression of p53 was shown to be elevated following the conditional deletion of Apc in the adult small intestine. Furthermore, p53 status was shown to impact on the transcription profile observed following Apc loss. A number of key Wnt pathway components and targets were altered in the p53 deficient environment. However, the aberrant phenotype observed following loss of Apc (rapid nuclear localisation of beta-catenin, increased levels of DNA damage, nuclear atypia, perturbed cell death, proliferation, differentiation and migration) was not significantly altered by the absence of p53. CONCLUSION: p53 related feedback mechanisms regulating Wnt signalling activity are present in the intestine, and become activated following loss of Apc. However, the physiological Wnt pathway regulation by p53 appears to be overwhelmed by Apc loss and consequently the activity of these regulatory mechanisms is not sufficient to modulate the immediate phenotypes seen following Apc loss. Thus we are able to provide an explanation to the apparent contradiction that, despite having a Wnt regulatory capacity, p53 loss is not associated with early lesion development.

2015-06-25 | GSE70262 | GEO

The impact of P53 loss on transcriptome changes following loss of Apc in the intestine

2015-06-25 | E-GEOD-70262 | biostudies-arrayexpress

Transcriptional profiling of intestinal changes following APC loss

Project description:Transcriptional profiling of intestinal changes following APC loss

| PRJNA445299 | ENA

Transcriptional profiling of intestinal changes following APC loss

Project description:Vil-CreERT2 was used to drive loss of APC (Adenomatous polyposis coli) in the murine intestinal epithelium. 4 days post induction, mice were sampled and 1cm of tissue from the proximal intestine was collected into RNA later. This was compared to control (wild-type) intestine. This analysis allows investigation of transcriptional changes following APC loss (and therefore activation of the WNT signalling pathway).

2019-12-31 | GSE112226 | GEO

Transcriptomic analysis of APC knockdown in proliferating primary myoblasts

Project description:APC is a key regulator of canonical Wnt signalling since it participates to beta-catenin targeting to proteasomal degradation when the pathway is inactive. Moreover, independently of Wnt signaling, APC regulates several cellular functions such as mycrotubule dynamics, chromosome segregation, cell adhesion. Although APC has been widely studied for its implication in initation and progression of several cancers, its role in satellite cells (skeletal muscle stem cells) has never been investigated. Here we used microarrays and to clarify APC functions and we identified several pathways and cellular processes to be affected following APC silencing.

2015-01-01 | GSE57898 | GEO

Proteomic changes in the colon of mice with epithelial specific deletion of Apc or Apc/Dhps

Project description:C57BL/6 mice with a tamoxifen (TAM)-inducible disruption of Apc (CDX2P-CreERT2;Apc fl/fl) or Apc and Dhps (CDX2P-CreERT2;Apc fl/fl;Dhps fl/fl) were injected or not with one intraperitoneal injection 4.5 mg/kg TAM. After 35 days, the following colon biopsies were harvested: normal tissues from mice that did not receive TAM, non-tumor tissues from mice treated with TAM, and tumors from mice treated with TAM. Biopsies from 5 mice were collected for the following sample groups: (1) Apc Control; (2) Apc/Dhps Control; (3) Apc+TAM non-tumor; (4) Apc/Dhps+TAM non-tumor; (5) Apc+TAM tumor; (6) Apc/Dhps+TAM tumor

2025-09-12 | PXD061596 | Pride

Gene expression data from the HCT-116 colon cancer cell line in the presence or absence of siRNA targeting APC

Project description:Gene expression data were collected by RNA-seq from HCT-116 cells in the presence or absence of siRNA targeting APC and 1,376 transcripts changed in expression following APC silencing were identified relative to scrambled siRNA-transfected and untreated controls.

2018-05-24 | GSE99263 | GEO

Transcription profiling of mouse liver in conditional knockouts of the tumor suppressor gene Apc identifies Wnt/bet-catenin signaling activation in hepatocytes

Project description:A "Cartes d'Identite des Tumeurs" (CIT) project from the french Ligue Nationale Contre le Cancer (http://cit.ligue-cancer.net). Conditional Knockout mouse model for the tumor suppressor gene Ap, Apc 2lox. The disruption of the Apc gene is realized by injection of an adenovirus encoding the Cre recombinase (AdCre). Homozygous animals injected with a high dose of AdCre showed an activation of the Wnt/beta-catenin signaling in most of the hepatocytes and develop an hepatomegaly and die soon after. Analysis of gene up- and down regulation for liver-indicible APC disruption.

2006-03-13 | E-TABM-81 | biostudies-arrayexpress

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