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Identification differentially expressed genes in the mouse testis caused by Prss55 deficiency

ABSTRACT: Identification differentially expressed genes in the mouse testis caused by Prss55 deficiency

PROVIDER: PRJNA419885 | ENA |

REPOSITORIES: ENA

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Identification differentially expressed genes in the mouse testis caused by Prss54 deficiency

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Project description:Testes from three wt and three Prss37-/- mice were used for gene expression analysis using whole mouse genome 4 × 44 k microarray (Agilent Technologies). A total of 31 genes were identified to be differentially expressed significantly (p < 0.05, fc ≥ 2). Fold changes of ten genes (seven up-regulated genes and three down-regulated genes) were examined by qRT-PCR for data validation.

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Project description:We applied iTRAQ Quantification Proteomics to explore Differentially expressed proteins between WT testis and Cfap70-KO testis. In this iTRAQ quantification project,there were 6 Mus_musculus samples named C_1, C_2, C_3, K_1, K_2, K_3 and we did 1 technical duplicate experiments. Totally 480850 spectrums were generated, 38748 peptides and 6734 proteins were identified with 1% FDR. proteins with 1.2 fold change(mean value of all comparison groups) and P-value(t-test of all comparision groups) less than 0.05 were defined as differentially expressed proteins.

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Identification of Differentially Expressed Genes in Human Testis Biopsies with Defective Spermatogenesis

Project description:The study was designed to identify differences in the expressed transcriptome of the two pathological human testis sample groups compared to normal spermatogenesis samples, with the objective of discovering genes related to the pathological condition. Transcriptional differences in human testis biopsies collected from men with normal spermatogenesis (NSP, n=3; intact germ cells, clinical score - 10), spermatid arrest (SDA, n=2; contains somatic cells and germ cells up to round spermatids, clinical score – 4-5) and Sertoli cell-only (SCO, n=2; complete absence of germ cells, clinical score - 0) phenotypes were assessed by RNA sequencing. A total of over 49 million reads were generated in NSP samples, over 24 million reads in SDA samples, and over 13 million reads in SCO samples. Differentially expressed genes (DEG) were identified based on a mean minimum count => 5, FDR <= 0.05 and log2FC fold change of >0.585 or <-0.585. Genes differentially expressed in pathological groups SDA and SCO compared to NSP, and genes differentially expressed in between the two pathological groups SDA and SCO were filtered. The study discovered a number of significantly differentially expressed genes (DEG) between groups as follows: NSP vs SDA n=1,873; SDA vs SCO n= 4,017; and NSP vs SCO n=10,253. A number of genes (n=10) were selected for further analysis by qRT-PCR and the detection of the protein (n=2) localization in testis by immunohistochemistry.

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