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Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse SMART-seq2]

ABSTRACT: Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse SMART-seq2]

PROVIDER: PRJNA497250 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8070100.fastq.gz	Fastqsanger.gz
	SRR8070110.fastq.gz	Fastqsanger.gz
	SRR8070120.fastq.gz	Fastqsanger.gz
	SRR8070130.fastq.gz	Fastqsanger.gz
	SRR8070140.fastq.gz	Fastqsanger.gz

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Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse SMART-seq2]

Project description:IL-10 is a prototypical anti-inflammatory cytokine, which is fundamental to the maintenance of immune homeostasis, especially in the intestine. There is an assumption that cells producing IL-10 have an immunoregulatory function. However, here we report that IL-10-producing CD4+ T cells are phenotypically and functionally heterogeneous. By combining single cell transcriptome and functional analyses, we identified a subpopulation of IL-10-producing Foxp3Neg CD4+ T cells that displays regulatory activity unlike other IL-10-producing CD4+ T cells, which are unexpectedly pro-inflammatory. The combinatorial expression of co-inhibitory receptors is sufficient to discriminate IL-10-producing CD4+ T cells with regulatory function from others and to identify them across different tissues and disease models in mice and humans. These regulatory IL-10-producing Foxp3Neg CD4+ T cells have a unique transcriptional program, which goes beyond the regulation of IL-10 expression. Finally, we found that patients with Inflammatory Bowel Disease (IBD), demonstrate a deficiency in this specific regulatory T-cell subpopulation.

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