Project description:Clones in the intestinal adult stem cell population mutant for Apc and Apc2, and over-expressing RasV12 and Snail are able to disseminate outside the gut and form macrometastases. These macrometastases can be cultured in allografts. This data shows the changes in gene expression profile of culturde tumors after 1, 2 and 10 (T1, T2, T10) rounds of allograft transplantation
Project description:Mutants in the Drosophila gene lethal (3) malignant brain tumor cause malignant growth in the larval brain. This data shows the changes in gene expression profile associated to mutations in l(3)mbt, both in situ in third instar larval brains and in tumors cultured for 1 5 and 10 (T1, T5, T10) rounds of allograft culture
Project description:Mutants in the Drosophila gene lethal (3) malignant brain tumor cause malignant growth in the larval brain. This data shows the changes in gene expression profile associated to mutations in l(3)mbt, both in situ in third instar larval brains and in tumors cultured for 1 5 and 10 (T1, T5, T10) rounds of allograft culture We performed genome-wide gene expression profiling of l(3)mbt[E2] and l(3)mbt[ts1] homozygous and transheterozygous larval brains raised at restrictive temperatures (29º C). We obtained three replicates for l(3)mbt[E2] at 17ªC (MBT_E2_17), l(3)mbt[ts1] at 17ºC (MBT_TS1_17), l(3)mbt[E2] at 29ºC (MBT_E2_29) and the transheterozygous at 29ºC (MBT_TS1_ME2), and six replicates l(3)mbt[ts1] at 29ºC (MBT_TS1_29). We also obtained three replicates for l(3)mbt[ts1] tumors at the first (T1), fifth (T5) and tenth (T10) round of allograft culture in adult flies, and six replicates for wild type w[1118] flies (WT).
Project description:To investigate the molecular mechanisms of pancreatic cancer metastasis, we performed proteomic profiling on clinical samples from metastatic and non-metastatic primary tumors. This approach aims to identify key drivers of tumor progression.
Project description:Metastasis to lymph nodes is an early and prognostically important event in the progression of many human cancers, and is associated with expression of vascular endothelial growth factor-D (VEGF-D). Changes to lymph node vasculature occur during metastasis, and may establish a metastatic niche capable of attracting and supporting tumor cells. We used microarrays to characterise the molecular profiles of endothelial cells from lymph nodes draining metastatic (VEGF-D-overexpressing) and non-metastatic tumors, and to identify differentially-expressed genes that might have therapeutic or prognostic potential. Draining lymph nodes of metastatic (VEGF-D-overexpressing) or non-metastatic tumors were pooled from 1-5 mice and enzymatically digested. Lymph nodes draining metastatic tumors were included for the analysis only if macroscopically enlarged, indicating the presence of metastatic cells. After digestion, tumor cells and leukocytes were depleted via immunomagnetic selection, and the resulting lymph node stromal cells were cultured briefly. Podoplanin was then used as a positive immunomagnetic selection marker to enrich for lymphatic and other endothelial cells in the lymph node. RNA was isolated from biological duplicate lymph node endothelial cell (LN EC) preparations and analysed by microarray.
Project description:This dataset contains mass spectrometry raw data (.d and wiff files) of primary and metastatic tumors of 16 localized colon cancer patients
Project description:<p>Metastatic breast tumors must adapt to the metabolic constraints of their target tissues. To understand how tumor metabolism varies across sites, we compared glucose utilization in primary mammary fat pad (MFP) tumors and brain metastases derived from the breast cancer cell line MDA-MB-231. Tumors in each site displayed distinct patterns of metabolite labeling, reflecting differences in nutrient availability and biosynthetic activity. Brain tumors exhibited higher labeling of amino acids and TCA cycle intermediates, consistent with increased synthesis to overcome restricted nutrient access in the brain, whereas MFP tumors showed greater nucleotide synthesis activity. These findings illustrate how the tumor microenvironment shapes metabolic adaptation and highlight the flexible strategies cancer cells use to maintain growth in diverse tissues.</p>