Project description:To investigate the molecular mechanisms underlying the altered hematopoietic support of mesenchymal stromal cells (MSC) expanded in human platelet lysate (hPL)-supplemented medium, we performed a comprehensive transcriptomic analysis comparing hPL-MSC and fetal bovine serum (FBS)-expanded MSC. MSC from three bone marrow donors were cultured under different adaptation regimens and co-cultured with hematopoietic stem and progenitor cells (HSPC). Transcriptomic analysis revealed distinct gene expression profiles, with 13% of genes differentially expressed between hPL-MSC and FBS-MSC, impacting cell signaling, extracellular matrix, and chemotaxis. Key pathways, including TGF-beta, PI3K-Akt, and Wnt signaling, were altered, affecting MSC-mediated HSPC expansion. hPL-MSC exhibited increased expression of inhibitory factors and reduced expression of promoters of HSPC proliferation, influencing Notch, TGF-β, JAK/STAT, and Wnt regulation. These findings highlight critical molecular interactions in MSC-HSPC co-culture and provide insights for optimizing xeno-free MSC expansion for hematopoietic applications.
Project description:Study designed to explore the effects of endothelial cell/MSC co-culture on individual gene expression profile of each cell type 4 independent samples from each of 4 groups: MSCs cultured alone; Pulmonary endothelial cells cultured alone; MSCs co-cultured with PECs then FACS separated; PECs co-cultured with MSCs then FACS separated
Project description:Mesenchymal stromal cells (MSC) were isolated from human bone marrow. Here, we have compared gene expression profiles of MSC at early and late passages. Long-term culture associated gene expression changes were then correlated with DNA-methylation profiles. The goal of this study was to determine if senescence-associated DNA-methylation (SA-DNAm) changes are reflected by differential gene expression. Overall, genes with SA-DNAm changes (particularly with SA-hypomethylation) were detected at low level and seemed to be scarcely expressed at early and late passages. MSC were isolated from three different donors and culture expanded until replicative senescence. Gene expression profiles were compared at early and late passage using GeneChip Humang Gene 1.0 ST arrays (Affymetrix). Six hybridizations are included in this series.
Project description:Mesenchymal stromal cells (MSC) were isolated from human bone marrow. Here, we have compared gene expression profiles of MSC at early and late passages. Long-term culture associated gene expression changes were then correlated with DNA-methylation profiles. The goal of this study was to determine if senescence-associated DNA-methylation (SA-DNAm) changes are reflected by differential gene expression. Overall, genes with SA-DNAm changes (particularly with SA-hypomethylation) were detected at low level and seemed to be scarcely expressed at early and late passages.
Project description:Mesenchymal stem cells (MSC) have emerged as potent therapeutic tool for a number of pathologies, including immune ones. However, unwelcome effects of MSC on the blood coagulation were revealed in some cases, which require more in-depth analysis. In this study, we explored the trombotic properties of human MSC from umbilical cord. We revealed strong procoagulant effects of umbilical cord MSC toward human and rat whole blood and platelets-free plasma using rotational thromboelastometry and thrombodynamics tests. The similar potentiation of clotting was demonstrated for MSC-derived extracellular vesicles (EV). In order to suggest approaches to avoid unwanted effects we studied the impact of heparin supplement on MSC/EV procoagulation properties. We found that therapeutic doses of unfractionated heparin injected in the patient's blood (administered in vivo) did not abrogate the procoagulant properties of MSC. Mass-spectrometry analysis of proteins of MSC and EV involved in coagulation-associated pathways was used to evaluate mechanisms of protrombotic effects.
Project description:The gene expression of mouse wt small intestinal epithelium and myofibroblast,3D organoids, both in monoculture and in co-culture, as well as small interstinal adenoma organoids from the Apc+/1638N mouse model, were measured by Affymetrix Mouse Gene 2.1 ST Array.
Project description:Airway epithelial cells can promote the proliferation of airway smooth muscle cells in an undisturbed environment. We isolated airway epithelial cells and airway smooth muscle cells in mice and conducted co-culture experiments. The results showed that the proliferative phenotype of airway smooth muscle cells was significantly changed by co-culture of epithelial cells after five day. Therefore, we conducted gene sequencing for ASMC in the co-culture group and ASMC in the non-co-culture group after 5 days of culture. The results show that Wnt pathway related genes are activated in ASMC, and the activation of this pathway may be the main reason why AEC stimulates the proliferation of ASMC