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Chronology of DNA methylation changes through transformation stage leading to senescence by-pass in Syrian hamster fetal primary cells exposed to benzo[a]pyrene

ABSTRACT: Chronology of DNA methylation changes through transformation stage leading to senescence by-pass in Syrian hamster fetal primary cells exposed to benzo[a]pyrene

PROVIDER: PRJNA604968 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11026976.fastq.gz	Fastqsanger.gz
	SRR11026977.fastq.gz	Fastqsanger.gz
	SRR11026978.fastq.gz	Fastqsanger.gz
	SRR11026979.fastq.gz	Fastqsanger.gz
	SRR11026980.fastq.gz	Fastqsanger.gz

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Chronology of DNA methylation changes through transformation stage leading to senescence by-pass in Syrian hamster fetal primary cells exposed to benzo[a]pyrene

Project description:Purpose: To use reduced representaion bisulfite sequencing to measure differential genome methylation among: 1. cells treated with benzo[a]pyrene (B[a]P) versus vehicle alone (DMSO) using the Syrian Hamster Cell Transformation Assay method; 2. cells originating from normal (Nc) versus morphologically transformed (MTc) colonies; 3. cells in senescence (SEN) versus those that have bypassed senescence (SENbp).

2023-02-10 | GSE144799 | GEO

DNA methylation changes from primary cultures through senescence-bypass in Syrian hamster fetal cells initially exposed to benzo[a]pyrene

Project description:This SuperSeries is composed of the SubSeries listed below. Purpose: Historically, the Syrian Hamster Embryo - cell transformation assay (SHE-CTA) has provided a method to predict the carcinogenicity of test chemicals. Note that the cells harvested for the SHE-CTA are from fetuses at gestation day 13.5 and not from embryos as historically recorded. This method has been criticized for insufficient mechanistic understanding and subjective assessment of colonies morphological transformation. A more objective method is needed that additionally provides mode of action information. A possible mode of action of carcinogens includes disruption of DNA methylation in gene regulatory regions and consequent changes in gene expression. Such genetic loci could provide biomarkers to assist in predicting the carcinogenicity of both genotoxic and non-genotoxic chemicals acting through DNA methylation disruption.

2023-02-10 | GSE220238 | GEO

Transcriptional profiling of Control vs. Pinealectomised Syrian hamster testes

Project description:Transcriptional profiling of male Syrian hamster testes, comparing control animals with those that have had a pinealectomy. Keywords: transcriptional profiling Two-condition experiment, Control vs pinealectomised animals

2010-05-26 | E-GEOD-10078 | biostudies-arrayexpress

DNA methylation in Syrian hamster female fetal cells (gestation day 13.5) exposed in vitro to 5-aza-2’deoxycytidine (5aCdR)

Project description:Purpose: To validate the reduced representation bisulfite sequencing (RRBS) methodology by comparing DNA methylation pattern in Syrian hamster fetal (SHF) primary cells to a SHF cell line (MT2) exposed to the DNA methyl transferase inhibitor,5aCdR. The MT2 cell line was originally derived from the expansion of a benzo[a]pyrene-induced morphologically transformed clone (Pickles et al 2016. Mutat.Res.Genet.Toxicol.Environ Mutagen. 802:50-58).

2023-02-10 | GSE144797 | GEO

Transcriptional profiling of Control vs. Pinealectomised Syrian hamster testes

Project description:Transcriptional profiling of male Syrian hamster testes, comparing control animals with those that have had a pinealectomy. Keywords: transcriptional profiling

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DNA methylation changes from primary cultures through senescence-bypass in Syrian hamster fetal cells initially exposed to benzo[a]pyrene

Project description:DNA methylation changes from primary cultures through senescence-bypass in Syrian hamster fetal cells initially exposed to benzo[a]pyrene

| PRJNA909278 | ENA

Glutathione S-transferase induction after exposure to benzo[a]pyrene

Project description:Benzo[a]pyrene induction of glutathione S-transferases, an activity-based protein profiling investigation

2020-05-26 | PXD010610 | Pride

Cytochrome P450 Monooxygenase Mediated Metabolic Utilization of Benzo(a)pyrene by Fungi

Project description:Soil dwelling Aspergillus fungi possess the versatile metabolic capability to utilize complex organic compounds which are toxic to humans, yet the mechanisms they employ remain largely unknown. Benzo(a)pyrene is a common carcinogenic contaminant, posing a significant concern for human health. Here, we report that Aspergillus fungi can degrade benzo(a)pyrene effectively. In Aspergillus nidulans, exposure to benzo(a)pyrene results in transcriptomic and metabolic changes associated with cellular growth and energy generation, implying that the fungus utilizes benzo(a)pyrene as a food. Importantly, we identify and characterize the conserved bapA gene encoding a cytochrome P450 monooxygenase that exerts the first step in the degradation of benzo(a)pyrene. We further demonstrate that the fungal NF-κB-type global regulators VeA and VelB are required for benzo(a)pyrene degradation in A. nidulans, which occurs through expression control of bapA in response to nutrient limitation. Our study illuminates fundamental knowledge of fungal benzo(a)pyrene metabolism and provides novel insights into enhancing bioremediation potential.

2018-07-10 | GSE116804 | GEO

Effect of the genotoxic human lung carcinogen benzo[a]pyrene and its non-carcinogenic isomer benzo[e]pyrene on transcriptomic profiles in HepaRG cells.

Project description:Twenty-eight days after the initial seeding, the terminally differentiated HepaRG cells were treated with 2 µM benzo[a]pyrene (B[a]P) and benzo[e]pyrene (B[e]P) for 72 hours. Following the treatment, the cells were harvested by mild trypsinization, washed in phosphate-buffered saline, and immediately frozen at −80°C for subsequent analyses. Gene expression profiles in the HepaRG cells treated with B[a]P and B[e]P were investigated using Agilent whole genome 8x60K human microarrays according to the manufacturer’s instructions.

2018-07-24 | GSE117476 | GEO

Acute hepatic transcriptional response in male mice following oral exposure to benzo(a)pyrene

Project description:Benzo(a)pyrene is a well-established human carcinogen in humans and rodents. In the present study, we sought to determine the dose- and time-dependent changes in gene expression upon oral exposure to benzo(a)pyrene. Adult male B6C3F1 mice were exposed to four doses of benzo(a)pyrene or vehicle control for three days and sacrificed 4 or 24 hours after the final exposure.

2015-07-01 | GSE43633 | GEO

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