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ABSTRACT: PacBio long-read sequencing on Dugesia japonica

PROVIDER: PRJNA627589 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR11594181_subreads.fastq.gz	Fastqsanger.gz
	SRR11594182_subreads.fastq.gz	Fastqsanger.gz
	SRR11594183_subreads.fastq.gz	Fastqsanger.gz
	SRR11594184_subreads.fastq.gz	Fastqsanger.gz
	SRR11594185_subreads.fastq.gz	Fastqsanger.gz

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Deep Sequencing identifies regulated small RNAs in Dugesia japonica

Project description:We applied Illumina sequencing to identify microRNAs (miRNAs) and piwi-interacting small RNAs (piRNAs) in Dugesia japonica. Dugesia were cut up after seven day’s starvation. DJ1 is the whole Dugesia body, DJ2 is the head part, DJ3 is the tail part, DJ4 is the left part and DJ5 is the right part. Total RNA was extracted by Trizol, and preserved in ethanol, stored at -80°C until further use.

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Project description:Here we describe CapTrap-Seq, an experimental workflow designed to address the problem of reduced transcript end detection by long-read RNA sequencing methods, especially at the 5' ends. We apply CapTrap-Seq to profile transcriptomes of the human heart and brain and we compared the obtained results with other library preparation approaches. CapTrap-Seq is a platform-agnostic method and here tested the method by using 3 different long-read sequencing platforms: MinION (ONT), Sequel (PacBaio) and Sequel II (PacBio).

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single cell RNA-Seq of Dugesia japonica

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