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Chronic restraint stress increases transcriptomic indicators of biological aging in mouse bone marrow leukocytes

ABSTRACT: Chronic restraint stress increases transcriptomic indicators of biological aging in mouse bone marrow leukocytes

PROVIDER: PRJNA660216 | ENA |

REPOSITORIES: ENA

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Chronic restraint stress increases transcriptomic indicators of biological aging in mouse bone marrow leukocytes

Project description:Genome-wide transcriptional profiling results were used to examine the effect of a psychological stress paradigm for rodents (chronic restraint) on indicies of biological aging in murine femoral bone marrow leukocytes and to assess the extent to which propranolol could block such effects.

2021-03-31 | GSE157133 | GEO

Chronic stress increases transcriptomic indicators of biological aging in mouse bone marrow leukocytes.

Project description:Research with animals and humans has demonstrated that chronic stress exposure can impact key biological aging pathways such as inflammation and DNA damage, suggesting a mechanism through which stress may increase risk for age-related disease. However, it is less clear whether these effects extend to other hallmarks of the aging process, such as cellular senescence. Male SCID mice were exposed to 14 days of restraint stress, with (n = 6) or without (n = 10) propranolol administration, or a non-stress control condition (n = 10). Normal femoral bone marrow leukocytes were isolated from engrafted leukemia cells that had been injected prior to the stressor, as the mice were also under a cancer challenge. We performed whole genome transcriptional profiling to assess indicators of biological aging: cell stress, DNA damage repair, cellular senescence markers p16INK4a and p21, and the pro-inflammatory senescence-associated secretory phenotype (SASP). ANCOVAs that adjusted for tumor load and Fisher's pairwise comparisons revealed that stressed mice had enhanced p16INK4a (p = .02) and p21 (p = .004), lower DNA damage repair (p < .001), and higher SASP (p = .03) gene expression than control mice. Stressed mice also showed up-regulated beta-adrenergic (CREB) and inflammatory (NF-кB, AP-1) and down-regulated cell stress (Nrf2) transcription factor activity relative to control mice (ps < .01). Propranolol reversed CREB and Nrf2 activity (ps < .03). Findings suggest that chronic stress exposure can impact several key biological aging pathways within bone marrow leukocytes and these effects may be partially mediated by sympathetic beta-adrenergic receptor activation.

| S-EPMC9035650 | biostudies-literature

Leukocytes

Project description:Leukocytes

| PRJNA576499 | ENA

Extracellular vesicles as prospective biological indicators for midgestational placental complications in the mouse

Project description:Extracellular vesicles as prospective biological indicators for midgestational placental complications in the mouse

| PRJNA1283131 | ENA

Microbial indicators

Project description:Microbial indicators for rehabilitation of post-minings substrate

| PRJEB10826 | ENA

Transgenerational effects of paternal restraint stress in Drosophila

Project description:At present, the experimental evidences of epigenetic inheritance caused by stresses are beginning to accumulate in some animal models. However, molecular mechanism and biological meaning underlying these phenomena has not been clearly understood yet. Previously, we showed that Drosophila transcription factor-2 (dATF-2) is involved in heterochromatin formation and that phosphorylation of dATF-2 by various stresses, such as heat shock and osmotic stresses, induces heterochromatin disruption. We also revealed these stress dependent epigenome changes mediated by dATF-2 can be inherited to next generations (Seong et al., 2011). In this study, we established a drosophila model of restraint stress (RS) to analyze the transgenerational effects of strong psychological stress. We observed that Drosophila F1 progenies, derived from RS exposed fathers, showed significant disruption of heterochromatin state and that such effect was not observed in the dATF-2 and MEKK1 mutant background. To reveal trangenerational effects and role of paternal restraint stress, we analyzed gene expression profiles of F1 progenies derived from either RS exposed or control fathers of wild-type and dATF-2 KO father.

2020-04-29 | GSE140950 | GEO

Characterization of leukocytes

Project description:Characterization of leukocytes

| PRJNA746993 | ENA

Biomarkers of hippocampal gene expression in a mouse restraint chronic stress model

Project description:Acute stress provides many beneficial effects whereas chronic stress contributes to a variety of human health problems. The objective of this study was to use a rodent model to uncover hippocampal gene signatures associated with prolonged chronic stress which could potentially serve as biomarkers and therapeutic targets for early diagnosis and pharmacological intervention for stress induced disease. Mice were subjected to restraint stress over 7 consecutive days and gene expression changes in the hippocampus were analyzed at 3, 12 and 24 hours following the final restraint treatment. Data indicated that mice exposed to chronic restraint stress exhibit a differential gene expression profile compared to non-stressed controls. The greatest differences were observed 12 and 24 hrs following the final stress test.

2016-11-02 | E-MTAB-2402 | biostudies-arrayexpress

Actvity-dependent profiling of lateral septum neurons following restraint stress

Project description:We used the phospho-TRAP protocol (Knight et al., 2011) to profile lateral septum populations that are activated following 1 hour of restraint stress.

2020-08-30 | GSE154749 | GEO

Leukocytes in non-tumor-bearing and tumor-bearing mice

Project description:To clarify the functional role of migratory liver-resident leukocytes (LRLs) in the pre-metastatic lung, we identify differentially expressed genes and address biological significance in the liver.

2018-06-22 | GSE76506 | GEO

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