Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Platelet microRNA bioinformatics analysis in patients with hepatocellular carcinoma

ABSTRACT: Platelet microRNA bioinformatics analysis in patients with hepatocellular carcinoma

PROVIDER: PRJNA665508 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12707564_1.fastq.gz	Fastqsanger.gz
	SRR12707564_2.fastq.gz	Fastqsanger.gz
	SRR12707565_1.fastq.gz	Fastqsanger.gz
	SRR12707565_2.fastq.gz	Fastqsanger.gz
	SRR12707566_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

Platelet microRNA bioinformatics analysis in patients with hepatocellular carcinoma

Project description:Purpose:To reveal platelet microRNAs（miRNAs） expression status and its potential clinical significance in patients with hepatocellular carcinoma(HCC) by bioinformatics analysis. Method: 5 patients (4 male, 1 female) with hepatocellular carcinoma, and 5 healthy individuals(3 male，2 female) as control from June 2019 to November 2019 were taken part in this cohort. The leukocyte—depleted platelets were prepared from the anticoagulated whole blood samples. The platelet RNA were extracted to construct miRNA libraries by using conventional gradient centrifugation, MACS and instructions of miRNeasy Mini Kit, performed data analysis and bioinformatics analysis after sequencing. Results: The DESeq2 package of R software was used to compare the gene expression level difference between the HCC group and the normal group. The cut-off criteria of differentially expressed miRNAs was |log2FC| >1 and p<0.05. Total 250 differentially expressed miRNAs were screened out, of which 111 miRNAs were down-regulated and 139 miRNAs were up-regulated. Gene Ontology(GO) analysis found that the differential miRNAs are involved in Positional Regulatory of Catabolic process, positional regulatory of cell catabolic process, negative regulation of phosphorylation, Wnt signaling pathway. Kyoto Encyclopedia of Genes and Genomes (KEGG)pathway analysis showed that platelet miRNAs are involved in a number of signal pathways, including FoxO signaling pathway, ubiquitin mediated proteolysis, axon guidance, autophagy-animal, TGF-beta signaling pathway. Conclusion:MiRNAs in platelets of patients with hepatocellular carcinoma were differentially expressed. Platelet miRNAs should be involved in the biological process of hepatocellular carcinoma, while more samples and molecular biological experiments are needed.

2021-09-21 | GSE158523 | GEO

microRNA profiling of human hepatocellular carcinoma

Project description:microRNA profiling of 89 human hepatocellular carcinoma tissues using bead-based technology. microRNA expression profiling was performed using freshly frozen hepatocellular carcinoma tissues obtained at the time of surgical treatment.

2011-02-17 | E-GEOD-20594 | biostudies-arrayexpress

microRNA profiling of human healthy liver and hepatocellular carcinoma

Project description:microRNA profiling of 10 human healthy liver and 9 human hepatocellular carcinoma tissues using bead-based technology microRNA expression profiling was performed using freshly frozen healthy liver and hepatocellular carcinoma tissues obtained at the time of surgical treatment. keywords: healthy liver, hepatocellular carcinoma, expression array, microRNA

2012-07-21 | E-GEOD-31383 | biostudies-arrayexpress

microRNA profiling of human hepatocellular carcinoma

Project description:microRNA profiling of 89 human hepatocellular carcinoma tissues using bead-based technology.

2011-02-17 | GSE20594 | GEO

MicroRNA expression profile in human hepatocellular carcinoma

Project description:MicroRNA expression profile in human hepatocellular carcinoma

| PRJNA477559 | ENA

mRNA expression profile modified by microRNA mir-517a (MIR517A) in human hepatocellular carcinoma cell line

2011-02-17 | GSE20679 | GEO

microRNA profiling of human healthy liver and hepatocellular carcinoma

Project description:microRNA profiling of 10 human healthy liver and 9 human hepatocellular carcinoma tissues using bead-based technology

2012-07-22 | GSE31383 | GEO

microRNA profile of human liver tissues: hepatocellular carcinoma vs. matched noncancerous liver tissue

Project description:microRNA profiling of hepatocellular carcinoma vs. matched noncancerous liver tissue 166 pairs of hepatocellular carcinoma and matched noncancerous liver tissues

2011-08-15 | E-GEOD-31384 | biostudies-arrayexpress

microRNA profile of human liver tissues: hepatocellular carcinoma vs. matched noncancerous liver tissue

Project description:microRNA profiling of hepatocellular carcinoma vs. matched noncancerous liver tissue

2011-08-16 | GSE31384 | GEO

mRNA expression profile modified by microRNA mir-517a (MIR517A) in human hepatocellular carcinoma cell line

Project description:mRNA expression profile modified by stable transfection of microRNA mir-517a (MIR517A) in a human hepatocellular carcinoma cell line Huh-7 Keywords: Hepatocellular carcinoma, Expression array, microRNA microRNA mir-517a (MIR517A) was transfected to Huh-7 cells using GFP-expressing lentiviral vector. Infected cells were selected using flow cytometry and subjected to mRNA expression microarray experiment. The profiles were compared to controls cells infected with only GFP protein.

2011-02-17 | E-GEOD-20679 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data