Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Exosomes differentiate mouse embryonic fibroblasts to beta-like cells

ABSTRACT: Exosomes differentiate mouse embryonic fibroblasts to beta-like cells

PROVIDER: PRJNA667440 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR12773630.fastq.gz	Fastqsanger.gz
	SRR12773631.fastq.gz	Fastqsanger.gz
	SRR12773632.fastq.gz	Fastqsanger.gz
	SRR12773633.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Exosomes differentiate mouse embryonic fibroblasts to beta-like cells

Project description:Diabetes is a concerning health malady worldwide. Islet or pancreas transplantation is the only long-term treatment available; however, the scarcity of transplantable tissues hampers this approach. Therefore, new cell sources and differentiation approaches are required. Apart from the genetic- and small molecule-based approaches, exosomes could induce cellular differentiation by means of their cargo, including miRNA. We developed a chemical-based protocol to differentiate mouse embryonic fibroblasts (MEFs) into β-like cells and employed mouse insulinoma (MIN6)-derived exosomes in the presence or absence of specific small molecules to encourage their differentiation into β-like cells. We further performed miRNA sequencing to identify the miRNAs present in the exosomes that could mediate the differentiation process. The miRNA sequencing study found some of the miRNAs to be highly upregulated in the exosomes, some in the parent MIN6 cells, and some other were present in almost equal representation. Some of the miRNAs were investigated for their role in mediating the differentiation process to β-like cells . These miRNAs were able to differentiate the exocrine cells too.

2020-12-01 | GSE159029 | GEO

Metabolomics Workbench ST000003 - GNPS - Metabolomic analysis of mouse embryonic fibroblasts, embryonic stem cells, and induced pluripotent stem cells

Project description:mouse embryonic fibroblasts, embryonic stem cells, and induced pluripotent stem cells were compared via metabolomic analysis

2018-08-02 | MSV000082680 | GNPS

Role of beta-actin in mouse embryonic fibroblasts (MEFs)

Project description:Role of beta-actin in mouse embryonic fibroblasts (MEFs)

| PRJNA630727 | ENA

microRNA sequencing in rat cardiac fibroblasts and fibroblast-derived exosomes

Project description:We found that cardiac fibroblasts produce and secrete exosomes. miRNA profiling and TaqMan qRT-PCR experiments identified miR-21 expression to be higher in cardiac fibroblasts compared to those of miR-21*, whereas in exosomes miR-21* expression was higher compared to miR-21. The purpose of the study was to validate these findings by miRNA sequencing in cardiac fibroblasts and fibroblasts-derived exosomes. Neonatal rat cardiac fibroblasts were cultured in DMEM + 1% exosome-depleted FBS for 48h. Conditioned medium was collected and exosomes were purified by several centrifugation and filtration steps, following ultracentrifugation. Afterwards total RNA from cardiac fibroblasts and exosomes was isolated for miRNA sequencing.

2015-12-19 | E-GEOD-76175 | biostudies-arrayexpress

ATAC-Seq analysis of beta actin knockout mouse embryonic fibroblasts expressing NLS-tagged beta-actin

Project description:ATAC-Seq analysis of beta actin knockout mouse embryonic fibroblasts expressing NLS-tagged beta-actin to study impact of actin levels on chromatin accessibility

2022-09-19 | GSE213431 | GEO

A549-derived exosomes transform fibroblasts into cancer-associated fibroblasts (microRNA)

Project description:The communication between tumor-derived exosomes and stroma plays a critical role in facilitating cancer progression. We detected the mRNA expression levels after MRC5 was transfected with shRNAs targeting FOXN3 or incubated with A549 cells-derived exosomes. We reported that in lung cancer microenvironment, tumor cells exhibit a great capacity to convert normal fibroblasts (NFs) to cancer-associated fibroblasts (CAFs). We showed that tumor cells secrete a subset of exosomal microRNAs directly targeting FOXN3, which leads to the activation of fibroblasts. Activated CAFs further promote cancer growth by entering tumor tissues. These results demonstrate that intercellular crosstalk between tumor cells and fibroblasts is mediated by tumor-derived exosomes, providing potential targets for treatment of lung cancer.

2025-12-31 | GSE144177 | GEO

A549-derived exosomes transform fibroblasts into cancer-associated fibroblasts (mRNA)

2025-12-31 | GSE144176 | GEO

Hippo deficient cardiac fibroblasts differentiate into osteochondroprogenitors (ST)

Project description:Hippo deficient cardiac fibroblasts differentiate into osteochondroprogenitors (ST)

| PRJNA1088152 | ENA

Hippo deficient cardiac fibroblasts differentiate into osteochondroprogenitors (snMultiomics)

Project description:Hippo deficient cardiac fibroblasts differentiate into osteochondroprogenitors (snMultiomics)

| PRJNA1088488 | ENA

A549-derived exosomes transform fibroblasts into cancer-associated fibroblasts (CUT&RUN)

Project description:With the in-depth study of exosomes, it has been discovered that tumor-derived exosomes could transform normal fibroblasts into cancer associated fibroblasts (CAFs) and further affect metastasis and drug resistance of CAFs. FOXN3 was the target of tumor-derived exosomes. The understanding of downstream genes regulated by FOXN3 is nessesary. An alternative ChIP-seq method, CUT&RUN was applied to explored the downstream genes directly regulated by FOXN3

2025-12-31 | GSE225116 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data