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Next Generation Sequencing Facilitates Quantitative Analysis of Il17ra villin cre -/+ colon transcriptomes at day 10 post C. rodentium infection

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of Il17ra villin cre -/+ colon transcriptomes at day 10 post C. rodentium infection

PROVIDER: PRJNA672119 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12901072_1.fastq.gz	Fastqsanger.gz
	SRR12901072_2.fastq.gz	Fastqsanger.gz
	SRR12901073_1.fastq.gz	Fastqsanger.gz
	SRR12901073_2.fastq.gz	Fastqsanger.gz
	SRR12901074_1.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing Facilitates Quantitative Analysis of Il17ra villin cre -/+ colon transcriptomes at day 10 post C. rodentium infection

Project description:We report analysis of colonic transcriptome in gut epithelial cells specific deficient mouse (Il17ra villin cre +) and littermate control mouse (Il17ra villin cre -) infected with Citrobater rodentium (C. rodentium) for 10 days. We found RNA-seq data show Nox family genes and defensin genes are downregulated in Il17ra villin cre + mice. It is interestingly Tnfsf13 was also downregulated in Il17ra villin cre + mice. These genes expressions were confirmed by qRT–PCR validation was performed using TaqMan assays. Finally, this study provides first detailed analysis of C. rodentium infected colonic transctiptome in Il17ra villin cre + mice, with biologic replicates, generated by RNA-seq technology.

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