Project description:A small population of telocytes (TC) have been identified in periodontium. They are quiescent in homeostasis, however, in periodontitis, they are activated by LPS via the receptor Lbp, which causes TCs to send HGF signals to the LPS activated iNOS+ (M1) macrophages. Macrophages receive the HGF signals via c-Met, which further causes the expression of M2 marker Arg1, representing in an increase of M2 macrophages. The expression of Arg1 can be inhibited by a HGF/c-Met selective inhibitor Tivantinib.
Project description:Skin resident macrophages were isolated by FACS sorting at different ages post natally and RNA was extracted mouse skin resident macrophages
Project description:Genes expression in Ly6C+/F4/80+ inflammatory macrophages, CX3CR1+/F4/80+ tissue resident macrophages and Ly6G+/F4/80- neutrophils which were isolated from day 3 wounds in C57/B6 mice aged 8 weeks by cell sorting Ly6C+ macrophages expressed higher (over 5 folds) levels of 241 genes compared to CX3CR1+ macrophages, and 3382 genes compared to neutrophils
Project description:Macrophages are essential in immune defence, tissue development, and disease onset and progression. Their function is in part dictated by their microenvironment and origin. Tissue-resident macrophages originate from yolk sac progenitors, persisting in adult organs and tissues mainly through self-renewal. Although ubiquitously present throughout the body, no dedicated macrophage population is known to function within the vast network of blood vessels, despite the importance of maintaining a healthy vascular and blood system. Here, we identified a distinctive population of macrophages residing within blood vessels, which we termed blood-resident macrophages (bMΦ). bMΦ actively survey the blood to eliminate foreign particles and unfit cells, and act as the first responders to endothelial damage. bMΦ emerge directly from the main axial vessels of zebrafish embryos though an atypical endothelial-to-macrophage transition (EMacT), independent of Runx1 and Csf1r, both essential for hematopoietic stem cell and myeloid cell production, respectively. Our data thus unveil the existence of a specialized population of macrophages that function within the confinement of the vasculature to safeguard blood from foreign threats and maintain blood vessel integrity. These discoveries not only expand our understanding of immune surveillance mechanisms but also offer potential avenues for the treatment of bloodborne diseases and vascular injuries.
Project description:microRNA transcriptome data from wild type and Gata6-deficient tissue resident peritoneal macrophages. Tissue resident macrophages are notoriously heterogeneous, exhibiting discrete phenotypes as a consequence of tissue- and micro-anatomical niche-specific functions, but the molecular basis for this is not understood. Gata6 itself has been shown to be a target of multiple miR. However, microRNA transcriptome and its dependence on tissue-specific macrophage programming, such as effected by GATA6, has not been explored. We used microRNA sequencing to determine the patterns of microRNA expression in peritoneal resident macrophages at homeostasis in the absence of GATA-6 against wild type.
Project description:Tissue resident macrophages are notoriously heterogeneous, exhibiting discrete phenotypes as a consequence of tissue- and micro-anatomical niche-specific functions, but the molecular basis for this is not understood. We resolved a restricted transcriptional profile for the self-renewing population of peritoneal resident macrophages, which is expressed during homeostasis and inflammation and distinct from other MM-CM-^X. Prominent within this profile was the expression of Gata6. This study represents a characterisation of the role of Gata6 in peritoneal resident macrophage phenotype. We used microarrays to determine the patterns of gene expression in peritoneal resident MM-CM-^X in the absence of GATA-6 against wild type. Conditional 'floxed' Gata6 deficient sex-matched mice between 7 weeks old were compared against wild type
Project description:It is commonly-recognized that hematopoietic cells residing in adipose tissue (AT) exhibit a broad range of physiological functions for maintenance of homeostasis and remodeling and repair of tissues aside from the involvement in inflammation. We evaluated the cell composition of hematopoietic cells in human non-obese AT and analyzed AT-resident macrophages in detail. Liposuction aspirates were obtained from healthy donors undergoing liposuction of the abdomen and thighs. CD34-positive AT resident macrophages (ATMs), CD34-negative ATMs and adipose stromal cells were sorted from aspirated AT by a multiparameter fluorescent-activated cell sorter. Circulating monocytes were also sorted from peripheral blood.These 4 condition were analysed transcription profile.
Project description:Although classified as hematopoietic cells, tissue-resident macrophages are selfrenewing and maintained independently of adult hematopoiesis. While most macrophages originate from embryonic precursors that seed tissues prior to birth, their exact origin is unknown. Using an in utero macrophage depletion strategy and fatemapping of yolk sac (YS) and fetal liver (FL) hematopoiesis, we found that YS macrophages are the main precursors of microglia, while most other macrophages derive from fetal monocytes. Both YS macrophages and fetal monocytes arise from erythro-myeloid progenitors (EMP) generated in the YS. In the YS, EMP gave rise to macrophages without monocytic intermediates, while EMP seeding the FL upon the establishment of blood circulation acquired c-Myb expression and gave rise to fetal monocytes that then seed embryonic tissues to differentiate into macrophages. Thus, adult tissue-resident macrophages established from HSC-independent embryonic precursors arise from two different developmental programs. 12 samples of progenitors, monocytes or macrophages are analyzed from 2 to 4 replicate. Each replicate derived from at least 5 embryos or adult mice
Project description:We show that resident macrophages accumulate in cilia mutant mice prior to cyst formation and that inhibition of resident macrophage accumulation reduces cystic kidney disease.
Project description:Periodontitis can impair the osteogenic differentiation of human periodontal mesenchymal stem cells, but the underlying molecular mechanisms are still poorly understood. Long noncoding RNAs (lncRNAs) have been demonstrated to play significant roles under both physiologic and pathological conditions. We performed comprehensive lncRNAs profiling by lncRNA microarray to identify differentially expressed long noncoding RNA expression between Periodontal ligament stem cells from healthy Periodontal tissue and periodontal ligament stem cells from inflammatory periodontal tissue. Our analysis identified 233 lncRNAs and 423 mRNAs that were differently expressed (fold change >2.0, p-value < 0.05) between the two groups of cells. The GO analysis revealed that the significantly down-regulated biological processes included multicellular organismal process, developmental process and multicellular organismal development and the significantly up-regulated biological processes included cellular process, biological regulation and response to stimulus in periodontal ligament stem cells from inflammatory periodontal tissue. The Pathway analysis revealed that the differentially expressed mRNAs may involved in Focal adhesion, ECM-receptor interaction, Bacterial invasion of epithelial cells, Long-term depression, Circadian entrainment and HIF-1 signaling pathway. Two-condition experiment, periodontal ligament stem cells from healthy periodontal tissue (hPDLSCs) vs. periodontal ligament stem cells from inflammatory periodontal tissue (pPDLSCs), Biological replicates: 3 control replicates (hPDLSCs), 3 testing replicates (pPDLSCs).