Project description:Foxtail millet (Setaria italica L. P. Beauv) has been considered as a tractable model crop in recent years due to its short growing cycle, lower repetitive DNA, inbreeding nature, small diploid genome, and outstanding abiotic stress-tolerance characteristics. With modern agriculture often facing various adversities, it’s urgent to dissect the mechanisms of how foxtail millet responds and adapts to drought and stress on the proteomic-level.
Project description:Transcriptome sequencing of Foxtail millet Setaria italica (Zhang-gu) for different tissues. Four RNA pools were created corresponding to four different tissues: root, leaf, stem, spica (tassel) at developmental stage, then each pool was sequenced.
Project description:To investigate the involvement of arbuscular mycorrhizal symbiosis in the moleular regulation in foxtail millet roots and the effects of genetic variation on AMS-mediated molecular regulation, we isolated total RNA from the roots of 3 different landraces for comprehensive transcriptomic analysis. We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 different landraces (Hanevalval, TT8, ICE36) after 6-week mock or arbuscular mycorrhizal fungi treatments.
2022-10-20 | GSE213843 | GEO
Project description:Transcriptome of foxtail millet
Project description:In Setaria italica (foxtail millet), SiUBC39 is implicated in regulating key agronomic traits, including plant height, flowering time, and stress tolerance. To elucidate the molecular mechanisms underlying SiUBC39’s roles in these traits, we affinity-purified SiUBC39-GFP and the corresponding GFP control protein via immunoprecipitation (IP). The immunoprecipitated complexes were then subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis to identify proteins interacting with SiUBC39.