Fusarium oxysporum
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ABSTRACT:
PROVIDER: PRJNA747720 | ENA |
REPOSITORIES: ENA
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Project description:<p>Saffron (Crocus sativus) is a high-value crop prized in culinary and pharmaceutical applications for its nutritional and therapeutic properties. However, corm rot caused by Fusarium oxysporum severely compromises yield and quality. Here, we isolated F. oxysporum strain CSP01 from diseased corms and confirmed it as the primary causal agent. Five endophytic fungi-CS05 (Trametes versicolor), CS06 (Bjerkandera adusta), CS07 (Phlebia acerina), CS33 (Penicillium sp.), and CS65 (Trichoderma sp.)-were screened for biocontrol efficacy against CSP01. CS05 showed the strongest suppression, achieving a 75.97% control rate and outperforming the positive controls Mortierella alpina and Trichoderma harzianum. To elucidate CS05-mediated protection, we integrated transcriptomic, proteomic, and metabolomic analyses. Transcriptome profiling revealed robust induction of host defense genes following CS05 treatment, particularly in phenylpropanoid biosynthesis (PAL2, 4CL1, CCR4), jasmonic acid (JA) signaling (COI1), and hydrogen peroxide (H2O2) metabolism (CAT1). Metabolomic analysis identified 461 differentially accumulated metabolites in CS05-treated corms; notably, L-tyrosine, coniferin, and p-coumaraldehyde were enriched within phenylpropanoid pathways and associated with enhanced resistance. qRT-PCR and enzyme activity assays validated the involvement of PAL2, 4CL1, and CCR4 in phenylpropanoid biosynthesis. Phytohormone measurements showed significantly elevated JA in CS05-treated corms (12.774 pmol/L) relative to pathogen-only corms (7.773 pmol/L). Oxidative stress assessments indicated that H2O2 in infected corms peaked at day 30 (41.189 mmolg-1 protein); at day 24, infected corms contained 20.904 mmolg-1 protein versus 16.760 mmolg-1 protein in CS05-treated corms, demonstrating mitigation of excessive reactive oxygen species. Collectively, these results show that T. versicolor CS05 enhances saffron resistance to corm rot by coordinating phenylpropanoid pathway activation, amplifying JA signaling, and improving H2O2 detoxification. This study provides the first evidence that T. versicolor CS05 is an effective, eco-friendly biocontrol agent for saffron corm rot and offers mechanistic insight into endophyte-driven plant defense, supporting its potential for sustainable disease management.</p>
2026-06-01 | MTBLS14647 | MetaboLights
Project description:Fusarium oxysporum causes Fusarium wilt syndrome in more than 120 different plant hosts, including globally important crops such as tomato, cotton, banana, melon, etc. F. oxysporum shows high host specificity in over 150 formae speciales and have been ranked in the top 10 plant fungal pathogens. Although three PMTs encoded by the pmt1, pmt2, and pmt4 are annotated in the genome of F. oxysporum, their functions have not been reported. As O-mannosylation is not found in plants, a comprehensive understanding of PMTs in F. oxysporum becomes attractive for the development of new strategy against Fusarium wilt. In order to understand the molecular mechanism of the differential functions of three PMTs, a comparative O-glycoproteome analysis of the pmt mutants were carried out.
2019-10-28 | PXD012530 | Pride
Project description:Root rot has severely affected the yield and quality of Polygonatum kingianum, and Fusarium oxysporum has been identified as the primary causal agent. To explore the virulence mechanisms of this pathogen, we performed a functional characterization of FoHsf1, a gene encoding a heat shock transcription factor in F. oxysporum. Using targeted gene knockout and complementation, phenotypic assays, and virulence tests, we found that the FoHsf1 deletion mutant (DEL) exhibited significant defects in mycelial growth, conidiation, and virulence relative to the wild-type (WT) and complemented (COM) isolates. Integrated transcriptomic and metabolomic profiling demonstrated that differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) were predominantly enriched in carbohydrate and amino acid metabolism pathways. More importantly, the metabolite abundance of caffeine and vitamin D2 was significantly reduced in DEL; exogenous application of these two metabolites partially restored fungal growth. These findings suggest that FoHsf1 regulates fungal development by modulating core metabolic pathways. Our results advance the current understanding of the molecular mechanism underlying FoHsf1-mediated virulence of F. oxysporum and offer potential targets for managing this disease.
2026-04-11 | MTBLS14221 | MetaboLights
Project description:Saffron (Crocus sativus) is a high-value crop prized in culinary and pharmaceutical applications for its nutritional and therapeutic properties. However, corm rot caused by Fusarium oxysporum severely compromises yield and quality. Here, we isolated F. oxysporum strain CSP01 from diseased corms and confirmed it as the primary causal agent. Five endophytic fungi-CS05 (Trametes versicolor), CS06 (Bjerkandera adusta), CS07 (Phlebia acerina), CS33 (Penicillium sp.), and CS65 (Trichoderma sp.)-were screened for biocontrol efficacy against CSP01. CS05 showed the strongest suppression, achieving a 75.97% control rate and outperforming the positive controls Mortierella alpina and Trichoderma harzianum. To elucidate CS05-mediated protection, we integrated tran scriptomic, proteomic, and metabolomic analyses. Transcriptome profiling revealed robust induction of host defense genes following CS05 treatment, particularly in phenylpropanoid biosynthesis (PAL2, 4CL1, CCR4), jasmonic acid (JA) signaling (COI1), and hydrogen peroxide (H2O2) metabolism (CAT1). Metabolomic analysis identified 461 differentially accumulated metabolites in CS05-treated corms; notably, L-tyrosine, coniferin, and p-coumaraldehyde were enriched within phenylpropanoid pathways and associated with enhanced resistance. qRT-PCR and enzyme activity assays validated the involvement of PAL2, 4CL1, and CCR4 in phenylpropanoid biosynthesis. Phytohormone measurements showed significantly elevated JA in CS05-treated corms (12.774 pmol/L) relative to pathogen-only corms (7.773 pmol/L). Oxidative stress assessments indicated that H2O2 in infected corms peaked at day 30 (41.189 mmolg-1 protein); at day 24, infected corms contained 20.904 mmolg- 1 protein versus 16.760 mmolg-1 protein in CS05-treated corms, demonstrating mitigation of excessive reactive oxygen species. Collectively, these results show that T. versicolor CS05 enhances saffron resistance to corm rot by coordinating phenylpropanoid pathway activation, amplifying JA signaling, and improving H2O2 detoxification. This study provides the first evidence that T. versicolor CS05 is an effective, eco-friendly biocontrol agent for saffron corm rot and offers mechanistic insight into endophyte-driven plant defense, supporting its potential for sustainable disease management.
2026-06-04 | PXD079291 |
Project description:The colonization of Capsicum annuum roots by Fusarium oxysporum Fo47 induces resistance responses on the plant. Fo47 is a non-pathogenic strain of Fusarium oxysporum. Fo47 colonizes only the most outer layers of the root surface but it does not colonize inner tissues. Pre-treatment of roots with Fo47 reduces the symptom development produced by later pathogen inoculation. The expression of genes in distal tissues was determined by microarray analysis of stems of Fo47-treated plants. Capsicum annuum samples were analyzed using Affymetrix chips of the close-related species Solanum lycopersicum.
2021-06-02 | GSE49432 | GEO
Project description:Fusarium oxysporum is one of the most common species causing soybean root rot and seedling blight in the U.S. In a recent study, significant variation in aggressiveness was observed among isolates of F. oxysporum collected from roots in Iowa, ranging from highly pathogenic to weakly or non-pathogenic isolates. In the present work, a RNA-seq-based analysis was used for the first time to investigate the molecular aspect of the interaction of a partially resistant soybean genotype with non-pathogenic/pathogenic isolates of F. oxysporum at 72 and 96 hours post inoculation (hpi). Markedly different gene expression profiles were observed in compatible and incompatible host-pathogen combinations. A peak of differentially expressed genes (DEGs) was observed at 72 hpi in soybean roots in response to both isolates, although the number of DEGs was about eight times higher for the pathogenic isolate compared to the non-pathogenic one (1,659 vs. 203 DEGs, respectively). Furthermore, not only the number of genes, but also the magnitude of induction was much greater in response to the pathogenic isolate. This response included a stronger activation of many well-known defense-related genes, and several genes involved in ethylene biosynthesis and signalling, transcription factors, secondary and sugar metabolism. In addition, 1130 fungal genes were differentially expressed between the F. oxysporum isolates in planta during the infection process. Interestingly, 10% of these genes encode plant cell-wall degrading enzymes, reactive oxygen species-related enzymes and fungal proteins involved in primary metabolic pathways. Such information may be useful in the development of new methods of broadening resistance of soybean to F. oxysporum, including the silencing of important fungal genes, and also to understand the molecular basis of soybean-F. oxysporum interactions. Soybean seedlings mRNA profiles inoculated with a non-pathogenic and pathogenic isolates of F. oxysporum and collected at 72 and 96 hpi, were generated using Illumina HiSeq 2500. Control seedlings were also included for each time of inoculation. Three biological replicates were considered for each condition, 18 samples in total.
2016-03-31 | E-GEOD-66861 | biostudies-arrayexpress
Project description:Crotonylated and secreted proteins in Kcr effectors in Fusarium oxysporum
2026-04-27 | PXD054345 | Pride
Project description:Fusarium oxysporum is one of the most common species causing soybean root rot and seedling blight in the U.S. In a recent study, significant variation in aggressiveness was observed among isolates of F. oxysporum collected from roots in Iowa, ranging from highly pathogenic to weakly or non-pathogenic isolates. In the present work, a RNA-seq-based analysis was used for the first time to investigate the molecular aspect of the interaction of a partially resistant soybean genotype with non-pathogenic/pathogenic isolates of F. oxysporum at 72 and 96 hours post inoculation (hpi). Markedly different gene expression profiles were observed in compatible and incompatible host-pathogen combinations. A peak of differentially expressed genes (DEGs) was observed at 72 hpi in soybean roots in response to both isolates, although the number of DEGs was about eight times higher for the pathogenic isolate compared to the non-pathogenic one (1,659 vs. 203 DEGs, respectively). Furthermore, not only the number of genes, but also the magnitude of induction was much greater in response to the pathogenic isolate. This response included a stronger activation of many well-known defense-related genes, and several genes involved in ethylene biosynthesis and signalling, transcription factors, secondary and sugar metabolism. In addition, 1130 fungal genes were differentially expressed between the F. oxysporum isolates in planta during the infection process. Interestingly, 10% of these genes encode plant cell-wall degrading enzymes, reactive oxygen species-related enzymes and fungal proteins involved in primary metabolic pathways. Such information may be useful in the development of new methods of broadening resistance of soybean to F. oxysporum, including the silencing of important fungal genes, and also to understand the molecular basis of soybean-F. oxysporum interactions.
2016-03-31 | GSE66861 | GEO
Project description:Upon exposure to unfavorable environmental conditions, plants need to respond quickly to maintain their homeostasis. For instance, physiological, biochemical and transcriptional changes occur during plant-pathogen interaction. In the case of Vanilla planifolia Jacks., a worldwide economically important crop, it is susceptible to Fusarium oxysporum f. sp. vanillae. This pathogen causes root and stem rot in vanilla plants that lead to plant death. To investigate how vanilla plants, respond at the transcriptional level upon infection with F. oxysporum f. sp. vanillae, here we employed the RNA-Seq approach to analyze the dynamics of whole-transcriptome changes during two-time frames of the infection. Analysis of global gene expression profiles indicated that the major transcriptional change occurred at 2 dpi, in comparison to 10 dpi. Whereas 3420 genes were found with a differential expression at 2 dpi, only 839 were identified at 10 dpi. The analysis of the transcriptional profile at 2 dpi suggests that, among other responses, vanilla plants prepare to counter the infection by gathering a pool of translational regulation-related transcripts. The screening of transcriptional changes of V. planifolia Jacks upon infection by F. oxysporum f. sp. vanillae provides insights into the plant molecular response, particularly the upregulation of ribosomal proteins at early stages. Thus, we propose that the plant-pathogen interaction between V. planifolia Jacks and F. oxysporum f. sp. vanillae causes a transcriptional reprogramming coupled with a translational regulation. Altogether, this study provides the identification of molecular players that could help to fight the most damaging disease of vanilla.
2019-11-19 | GSE134155 | GEO
Project description:Deep sequencing of mRNA from Fusarium oxysporum f. sp. Cubense 1 and 4 after infecting Musa acuminata 0h and 48h.
2014-08-27 | GSE40581 | GEO