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ABSTRACT: Bulk RNA-seq of mouse epididymal adipose tissue

PROVIDER: PRJNA847010 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19577733.fastq.gz	Fastqsanger.gz
	SRR19577734.fastq.gz	Fastqsanger.gz
	SRR19577735.fastq.gz	Fastqsanger.gz
	SRR19577736.fastq.gz	Fastqsanger.gz
	SRR19577737.fastq.gz	Fastqsanger.gz

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The Nuclear Receptor Rev-erb alpha Regulates Adipose Tissue-Specific FGF21 Signaling (ChIP-seq, eWAT)

Project description:The goal of this study is to identify the cistrome of the transcriptional repressor Rev-erb alpha in epididymal white adipose tissue. Performing Rev-erb alpha ChIP-seq on epididymal white adipose tissue from wildtype mice at 5PM when Rev-erb alpha protein level peaks in wild type (WT) mice, we were able to globally determine the genomic regions undergoing Rev-erb alpha-dependent de-repression. Examination of Rev-erb alpha binding in epididymal white adipose tissue.

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Project description:Here we have employed RNA-seq to profile the transcriptional landscapes of in vitro differentiated primary mouse adipocytes isolated from epididymal and inguinal white adipose tissus as well as brown adipose tissue.

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Project description:Here we have employed DNase-seq combined with deep sequencing to map and compare DNase hypersensitive sites in in vitro differentiated primary mouse adipocytes isolated from epididymal and inguinal white adipose tissus as well as brown adipose tissue.

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Project description:Bulk RNA-seq was performed on fibroblasts and mesothelial cells from 11 different healthy mouse tissues such as bone, epididymal, brown and inguinal fat pads, mesentery, omentum, pancreas, liver, lung, lymph node and spleen. This data was used to examine tissue specific gene expression programs of fibroblasts and mesothelial cells.

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Project description:Bulk ATAC-seq was performed on fibroblasts from 8 different healthy mouse tissues such as bone, epididymal and inguinal fat pads, omentum, liver, lung and lymph node. This data was used to examine tissue specific chromatin landscapes in fibroblasts.

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Project description:To search long noncoding RNAs (lncRNAs) which regulating energy metabolism in high fat diet induced T2DM mouse, we performed transcriptome analyses to simultaneously profile mRNAs and lncRNAs in epididymal adipose tissue in normal and high fat diet induced mouse. Combining genome-wide screens, bioinformatics function predictions, and cell-based analyses, we developed an integrative roadmap to identify lncRNA metabolic regulators in epididymal adipose tissue under high fat diet induced T2DM mouse.

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