Project description:Iron induces hepcidin by activating bone morphogenetic protein (BMP)6-SMAD signaling. Liver endothelial cells (LECs) produce BMP6, but the molecular mechanisms are incompletely understood. To address this, we performed proteomics and RNA-sequencing on LECs from iron-adequate and iron-loaded mice. Gene set enrichment analysis identified transcription factors activated by high iron, including Nrf-2, which was previously reported to contribute to BMP6 regulation, and proto-oncogene c-Jun (encoded by Jun). Jun knockdown blocked Bmp6, but not Nrf-2 pathway, induction by iron in LEC cultures. Moreover, chromatin immunoprecipitation of mouse livers showed iron-dependent c-Jun binding to predicted sites in Bmp6 regulatory regions. Finally, c-Jun inhibitor blunted induction of Bmp6 and hepcidin, but not Nrf-2 activity, in iron-loaded mice. However, Bmp6 expression and iron parameters were unchanged in endothelial Jun knockout mice. Our data suggest that c-Jun participates in iron-mediated BMP6 regulation independent of Nrf-2, though the mechanisms may be redundant and/or multifactorial.

2023-12-28 | PXD039830 | Pride

Landscape of CD4+Foxp3+ Treg cells in the liver of neonatal mice

Project description:To study the heterogeneity of regulatory T cells (Tregs) in various organs, we performed single-cell RNA sequencing on liver and splenic Treg cells obtained from 12-day and 7-week old mice. Unique Treg subsets in neonatal liver were found and their impact on liver development and maturation was investigated.

2025-07-05 | GSE208263 | GEO

Connexin43 null vs wildtype neonatal mouse HEART

Project description:Cy3-labeled cDNA obtained from four pools of three hearts of neonatal C57BL Cx43 null mice were compared to Cy3-labeled cDNA obtained from four pools of three hearts of neonatal C57BL wildtype mice through Cy5-labeled sample reference prepared at once for the entire experiment from aorta, brain, heart, kidney, liver, lung, ovary/testicles, spleen, and stomach - equal amounts from adult male and female C57BL mice. Keywords = Cx32 null vs wildtype neonatal mouse heart

2010-06-05 | E-GEOD-1961 | biostudies-arrayexpress

Exosomal proteins identification from neonatal mouse plasma

Project description:Paracrine effects mediated by exosomes contribute to the major regenerative functions of cell therapies after myocardial injuries. Early neonatal mammals retain a transient regeneration capacity after heart injury, which reminds us to explore the cardioprotective and regenerative potency of plasma exosomes from neonatal mouse (npEXO). Therefore, we isolated plasma exosomes from neonatal mice and performed proteomic analysis.

2022-12-13 | PXD038805 |

RNA-seq analysis of neonatal mouse cochlear supporting cells

Project description:RNA-seq analysis of neonatal mouse cochlear supporting cells

| PRJNA325664 | ENA

Connexin43 null vs wildtype neonatal mouse HEART

2004-11-13 | GSE1961 | GEO