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Identification of the factor to lead human mesenchymal stem cell line into decellularized bone

ABSTRACT: Identification of the factor to lead human mesenchymal stem cell line into decellularized bone

PROVIDER: PRJNA862460 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR20649200_1.fastq.gz	Fastqsanger.gz
	SRR20649200_2.fastq.gz	Fastqsanger.gz
	SRR20649201_1.fastq.gz	Fastqsanger.gz
	SRR20649201_2.fastq.gz	Fastqsanger.gz
	SRR20649202_1.fastq.gz	Fastqsanger.gz

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SRRM4 knockout helps the human mesenchymal stem cell line to intrude into decellularized bone

Project description:Analysis of the human bone marrow microenvironment requires an in vivo model that reflects this microenvironment. We introduced a human bone marrow mesenchymal stem cell (MSC) line into decellularized bone. Using the CRISPR Knock Out library, we identified serine/arginine repetitive matrix 4 knockout in an MSC cell line, as a candidate factor for bone intruding, which may be applied to the analysis of hematological diseases such as myelodysplastic neoplasm.

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SRRM4 knockout helps the human mesenchymal stem cell line to intrude into decellularized bone

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Identification of the factor to lead human mesenchymal stem cell line into decellularized bone

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Identification of the Factor That Leads Human Mesenchymal Stem Cell Lines into Decellularized Bone.

Project description:Hematopoiesis is maintained by the interaction of hematopoietic stem cells (HSCs) and bone marrow mesenchymal stem cells (MSCs) in bone marrow microenvironments, called niches. Certain genetic mutations in MSCs, not HSCs, provoke some hematopoietic neoplasms, such as myelodysplastic syndrome. An in vivo bone marrow niche model using human MSC cell lines with specific genetic mutations and bone scaffolds is necessary to elucidate these interactions and the disease onset. We focused on decellularized bone (DCB) as a useful bone scaffold and attempted to induce human MSCs (UE7T-9 cells) into the DCB. Using the CRISPR activation library, we identified SHC4 upregulation as a candidate factor, with the SHC4 overexpression in UE7T-9 cells activating their migratory ability and upregulating genes to promote hematopoietic cell migration. This is the first study to apply the CRISPR library to engraft cells into decellularized biomaterials. SHC4 overexpression is essential for engrafting UE7T-9 cells into DCB, and it might be the first step toward creating an in vivo human-mouse hybrid bone marrow niche model.

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Project description:The aim of this study was to identify non-coding RNA that were rapidly differentially expressed after loss of anchorage in human bone marrow-mesenchymal stromal cells (MSCs).

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