Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

AoSte12 is required for mycelial development, conidiation, trap morphogenesis, and secondary metabolism by regulating hyphal fusion in nematode-trapping fungus of Arthrobotrys oligospora [gene expression]

ABSTRACT: AoSte12 is required for mycelial development, conidiation, trap morphogenesis, and secondary metabolism by regulating hyphal fusion in nematode-trapping fungus of Arthrobotrys oligospora [gene expression]

PROVIDER: PRJNA880859 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21591311_1.fastq.gz	Fastqsanger.gz
	SRR21591311_2.fastq.gz	Fastqsanger.gz
	SRR21591312_1.fastq.gz	Fastqsanger.gz
	SRR21591312_2.fastq.gz	Fastqsanger.gz
	SRR21591313_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

TheArf‐GAPProteins AoAge1 and AoAge2 are Required for Mycelial Growth, Conidiation, Stress Responses, Trap Formation, and the Secondary Metabolism inthe Nematode-Trapping Fungus Arthrobotrys oligospora

Project description:ADP-ribosylation factors (Arfs) belong to the small GTPases superfamily and regulate mycelial development, endocytosis, and virulence in fungi. Nematode-trapping (NT) fungi can form unique infection structures (traps) to capture and kill free-living nematodes, thus play a potential role in the biocontrol of nematodes. Arthrobotrys oligospora is a representative species of the NT fungi. Here, the functions of two Arf-GAPs, Age1 (AoAge1) and Age2 (AoAge2) orthologue to S. cerevisiae were identified in A. oligospora by gene knockout and multi-phenotypic analysis. It was found that AoAge1 and AoAge2, especially AoAge2, play an important role in vegetative growth, conidiation, trap formation, DNA damage, endoplasmic reticulum stress, mitochondrial activity, endocytosis, heat shock stress, ROS level, and autophagy of A. oligospora. Importantly, our transcriptomic analysis (12 h) showed that nearly 62.7% of A. oligospora genes (11479) were directly or indirectly regulated by AoAge2, indicating that Arf-GAP plays a global role in the growth and development of A. oli gospora. Revealed by transcriptomic analysis differentially upregulated genes in the absence of Aoage2 were involved in autophagy and carbohydrate, amino acid and sphingolipid metabolism while downregulated genes were involved in longevity regulating pathway and carbohydrate, amino acid and secondary metabolites biosynthesis. In addition, metabolomic analysis showed that many compounds were markedly reduction in ΔAoage2 mutants strain. Our results highlighted that AoAge1 and AoAge2 play a crucial role in the asexual growth, development, differentiation, and lifestyle transition, especially, AoAge2 is indispensable for conidiation and trap morphogenesis of A. oligospora. These results demonstrated crucial roles for AoAge1 and AoAge2 in the fungal growth, environmental adaption and pathogenicity, which provided insights into the function of Arf-GAPs in A. oligospora and other fungi.

2024-01-26 | GSE253759 | GEO

The Arf‐GAP Proteins AoAge1 and AoAge2 are Required for Mycelial Growth, Conidiation, Stress Responses, Trap Formation, and the Secondary Metabolism in the Nematode-Trapping Fungus Arthrobotrys oligospora

2022-10-13 | GSE215079 | GEO

AoSte12 is required for mycelial development, conidiation, trap morphogenesis, and secondary metabolism by regulating hyphal fusion in nematode-trapping fungus of Arthrobotrys oligospora [gene expression]

Project description:Transcriptomic analysis revealed that differentially expressed genes in the absence of AoSte12 were involved in mycelial growth, development process,conidiation, cell nucleus and trap formation.

2023-01-29 | GSE213447 | GEO

AoMbp1 interacts with AoSwi6 and plays pleiotropic roles in mycelial development, trap morphogenesis, and stress respone in the nematode-trapping fungus Arthrobotrys oligospora [RNA-Seq]

Project description:The fungal specific APSES family proteins involve in regulating fungal growth, development, and multiple biological processes. In this study, AoMbp1, an ortholog of Saccharomyces cerevisiae APSES-type transcription factor Mbp1, was functionally analyzed in a representative nematode-trapping fungus Arthrobotrys oligospora. Inactivation of Aombp1 caused a severe affect on the mycelial growth and development, the mycelial growth rate of ∆Aombp1 mutant was remarkably decreased, the hyphal septa were increased whereas the number of nuclei were significantly reduced, and the lipid droplet accumulation was remarkably increased. Meanwhile, the deletion of Aombp1 resulted a considerable reduction in the number of conidiophores and spore yield, which also caused abnormal spore morphology. In addition, the ∆Aombp1 mutants became more sensitive to several chemical stressors, especially to hyperosmotic reagents. Importantly, disruption of Aombp1 caused the number of traps and nematode-trapping ability were significantly reduced, and most of the traps have changed from their original three-dimensional structure to a planar shape. RNA-Seq, DAP-Seq and Y2H assay showed that AoMbp1 interacted with AoSwi6, and involved in regulating cell cycle, meiosis, lipid metabolism, DNA replication, mismatch repair and nucleotide excision repair. Our study elucidated the functions and potential regulatory mechanism of APSES protein Mbp1 in the mycelial development and trap morphogenesis of nematode-trapping fungi.

2025-09-11 | GSE305351 | GEO

AoSte12 is required for mycelial development, conidiation, trap morphogenesis, and secondary metabolism by regulating hyphal fusion in nematode-trapping fungus of Arthrobotrys oligospora [DAP-seq]

Project description:AoSte12 is required for mycelial development, conidiation, trap morphogenesis, and secondary metabolism by regulating hyphal fusion in nematode-trapping fungus of Arthrobotrys oligospora [DAP-seq]

| PRJNA880858 | ENA

AoSho1 and AoOpy2 are involved in the regulation of mycelial growth, sporulation, osmoregulation, and pathogenicity in Arthrobotrys oligospora

Project description:Arthrobotrys oligospora, a widely distributed nematode-trapping fungus, utilises adhesive mycelial nets (traps) to capture nematodes. As key components of the MAPK cascade, Sho1 and Opy2 are critical in the fungal stress response. This study examined the roles of homologous Sho1 (AoSho1) and Opy2 (AoOpy2) through gene knockdown, phenotypic analysis, and multi-omics approaches. The results revealed that knockdown of Aosho1 and Aoopy2 led to reduced mycelial growth, a significant decrease in spore production, trap formation, and nematode predation capacity. Furthermore, deletion of Aosho1 and Aoopy2 increased autophagic activity and heightened sensitivity to osmotic stress. Transcriptome analysis indicated that AoOpy2 functions as a multifaceted regulator in fungal growth, development, and environmental adaptation. Metabolomics data also suggested that AoSho1 and AoOpy2 are involved in several metabolic pathways. In conclusion, AoSho1 and AoOpy2 are essential for mycelial growth, osmoregulation, and the pathogenicity of A. oligospora. This study lays the groundwork for understanding the roles and potential mechanisms of the MAPK signalling pathway in the development and pathogenicity of nematode-trapping fungi.

2025-08-27 | GSE306156 | GEO

Aoatg11 and Aoatg33 are indispensable for mitophagy, and contribute to conidiation, the stress response, and pathogenicity in the nematode-trapping fungus Arthrobotrys oligospora

Project description:Mitophagy is one of the most important cellular processes to ensure mitochondrial quality control, which aims to transport damaged, dysfunctional, or excess mitochondria for degradation and reuse. Here, we determined the function of AoAtg11 and AoAtg33, two orthologous autophagy-related proteins involved in yeast mitophagy, in the typical nematode-trapping fungus Arthrobotrys oligospora . Deletion of Aoatg11 and Aoatg33 impairs mitophagy, mitochondrial morphology and activity, autophagy,cell apoptosis, reactive oxygen species levels, lipid droplet accumulation, and endocytosis. These combined effects resulted in slow vegetative growth; reduced conidiation, trap formation, cell nucleus, and extracellular protease activity; increased susceptibility to the stress response; and arthrobotrisin production in the Δ Aoatg11 and Δ Aoatg33 mutants, compared with the wild-type strain. In addition, the absence of Aoatg11 caused an endoplasmic reticulum stress response. Transcriptome analysis revealed that many differentially expressed genes in the Δ Aoatg11 mutants were involved in various important cellular processes, such as lipid metabolism, the TCA cycle, mitophagy, nitrogen metabolism, endocytosis, and the MAPK signaling pathway. In conclusion, our study revealed that Aoatg11 and Aoatg33 mediate autophagy and mitophagy in A. oligospora , and provides a basis for elucidating the links between mitophagy and fungal vegetative growth, conidiation, and pathogenicity.

2022-09-09 | GSE212735 | GEO

AoMedA has complex regulatory relationship with AoBrlA, AoAbaA and AoWetA in conidiation, trap formation, and secondary metabolism in nematode-trapping fungus Arthrobotrys oligospora

Project description:Transcriptomic analysis revealed that differentially expressed genes in the absence of AomedA and AoWetA were involved in mycelial growth, development process,conidiation, cell nucleus, trap formation, peroxisome, endocytosis and lipid metabolism.

2023-07-05 | GSE230299 | GEO

Genome-wide expression profiling of nematode-trapping fungus Arthrobotrys oligospora TWF154 upon nematode exposure

Project description:We report the transcriptomic comparisions between key processes required for various stages of fungal carnivory in nematode-trapping fungus Arthrobotrys oligospora when induced with nematodes. The reference assembly used for remapping is A. oligospora TWF154 (GenBank assembly accession: GCA_004768765.1)

2023-07-01 | GSE233568 | GEO

Penicillium marneffei: Gene expression profiling of hyphal and yeast cells and during conidiation

Project description:Penicillium marneffei (Talaromyces marneffei) is an opportunistic human pathogen that can grow in a multicellular hyphal form at 25M-BM-0C or a unicellular fission yeast form at 37M-BM-0C, and can switch between these two forms in response to temperature. The yeast form is found in infected individuals and represents the pathogenic phase. In response to specific environmental cues, the hyphal form can undergo asexual development (conidiation) the produce a differentiated multicellular structure called a conidiophore which produces dormant spores (conidia). Inhaled conidia initiate infection. To identify genes that are M-bM-^@M-^Xphase or cell-state specificM-bM-^@M-^Y transcriptional profiling was performed with a custom microarray consisting of ~42% of the predicted P. marneffei genes and RNA from P. marneffei hyphal, yeast and conidiation cell types. A custom microarray consisting of short, random genomic fragments from P. marneffei (~42% of the predicted genes) was generated using PCR products of the inserts from two independent DNA libraries constructed from genomic DNA of the P. marneffei type strain FRR2161. PCR products from previously cloned P. marneffei genes with known expression profiles were included as controls on the microarray. Total RNA from hyphal, yeast or conidiation cell types was prepared and used in pairwise combinations (conidiation versus hyphal cells, conidiation versus yeast cells and hyphal versus yeast cells) on the microarrays. Three biological replicate cultures were prepared for each experiment.

2013-11-21 | E-GEOD-51109 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data