Project description:To identify mediators of the osmotic stress response in Mycobacterium tuberculosis (Mtb), we performed transcriptional profiling of WT CDC1551 following treatment with 140 mM NaCl for 1 hr relative to an untreated control. 140 mM NaCl was chosen to reflect the approximate osmolarity of human plasma (i.e., 280 mOsm/L), which may be relevant during the course of infection.
Project description:To identify mediators of the osmotic stress response in Mycobacterium tuberculosis (Mtb), we performed transcriptional profiling of WT CDC1551 following treatment with 140 mM NaCl for 1 hr relative to an untreated control. 140 mM NaCl was chosen to reflect the approximate osmolarity of human plasma (i.e., 280 mOsm/L), which may be relevant during the course of infection. CDC1551 was treated with 140 mM NaCl for 1 h and compared to the same strain treated with 0 mM NaCl for 0 h. 4 biological replicates, independently grown and harvested. One replicate per array.
Project description:To characterize the role of Rv0516c in the osmotic stress response of Mycobacterium tuberculosis (Mtb), we performed transcriptional profiling of CDC1551 Rv0516c::Tn following treatment with 140 mM NaCl for 1 hr relative to an untreated control.
Project description:To characterize the role of Rv0516c in the osmotic stress response of Mycobacterium tuberculosis (Mtb), we performed transcriptional profiling of CDC1551 Rv0516c::Tn following treatment with 140 mM NaCl for 1 hr relative to an untreated control. CDC1551 Rv0516c::Tn was treated with 140 mM NaCl for 1 h and compared to the same strain treated with 0 mM NaCl for 0 h. 4 biological replicates, independently grown and harvested. One replicate per array.
