Project description:The purpose of this experiment was to determine what gene expression changes are induced by acute exercise in humans, and how those changes relate to insulin sensitivity 14 subjects had muscle biopsies at rest and 30 minutes after a single exercise bout for measurement of mRNA changes. Glucose clamps were used to assess insulin sensitivity.
Project description:In this study, the primary objective was to characterise the impact of regular post-exercise (20 strength training sessions across 10 weeks) cold-water immersion (CWI) on DNA methhylation. Secondary to this, the effect of regular post-exercise CWI on strength gains and post-exercise soreness was investigated. We used microarrays to detail the global effects of CWI on DNA methylation in vastus lateralis muscle tissue.
Project description:In this study, the primary objective was to characterise the impact of regular post-exercise (20 strength training sessions across 10 weeks) cold-water immersion (CWI) on mRNA expression. Secondary to this, the effect of regular post-exercise CWI on strength gains and post-exercise soreness was investigated. We used microarrays to detail the global effects of CWI on gene expression in vastus lateralis muscle tissue.
Project description:While moderate endurance exercise has been reported to improve cardiovascular health , its effects on cardiac structure and function are not fully characterized, especially with respect to sex dimorphism. We aimed to assess the effects of moderate endurance exercise on cardiac physiology in male versus female mice.
Project description:Exercise-induced fatigue and exhaustion have been an interesting area for many physiologists.Muscle glycogen is critical forphysical performance. However, how glycogen depletion is manipulated during exercise is not very clear. Our aim here is to assess the impact of interferon regulatory factor 4 (IRF4) on skeletal muscle glycogen and subsequent regulation ofexercise capacity. Skeletal muscle-specific IRF4 knockout mice show normal body weight and insulin sensitivity, but better exercise capacity and increased glycogen content with unaltered triglyceride levels compared to control mice on chow diet. In contrast, mice overexpression of IRF4 display decreased exercise capacity and lower glycogen content. Mechanistically, IRF4 regulates glycogen-associated regulatory subunit protein targeting to glycogen (PTG) to manipulate glucose metabolism. Knockdown of PTG can reverse the effects imposed by the absence of IRF4in vivo. Our studies reveal a regulatory pathway including IRF4/PTG/glycogen synthesis that controlling exercise capacity.