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UnknownAbsolute quantitative proteomics using the total protein approach to identify novel clinical immunohistochemical markers in renal neoplasms.
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S-EPMC8420025 | biostudies-literature
ProteomicsCompatibility of distinct label-free proteomic workflows in absolute quantification of proteins linked to the oocyte quality in human follicular fluid (the Quad-Orbitrap workflow)
We present a comprehensive analysis of human follicular fluid proteome by comparing several strategies of sample preparation, mass spectrometry analysis, and data processing, resulting in the identification of 1638 proteins. We devised two separate label-free quantitative workflows, termed after the...
ORGANISM(S): Homo sapiens (Human) 
2021-07-23 | PXD024347 | Pride
Lc-ms/ms Oocyte quality control Human follicular fluid Oocyte maturity Total protein approach Proteome Blastocyst development 
ProteomicsQuantitative Analysis of of Human Liver
Large scale proteomic analysis of human liver from 10 donors
ORGANISM(S): Homo sapiens (Human) 
2018-04-23 | PXD003458 | Pride
Human liver  total protein approach  med-fasp  fasp 
ProteomicsComprehensive and at copy number resolution analysis of the red blood cells proteome unravels unexpected membrane transporters
Red blood cells (RBC) are the most abundant cell type in human body that are responsible not only for tissue transport of oxygen and carbon dioxide, but also for a range of other functions. RBC and, in particular, their plasma membrane have been extensively studied for many years. These investigatio...
ORGANISM(S): Homo sapiens (Human) 
2017-08-11 | PXD005200 | Pride
"total protein approach" Human red blood cell Fasp 
ProteomicsCompatibility of distinct label-free proteomic workflows in absolute quantification of proteins linked to the oocyte quality in human follicular fluid (the Triple-Quad TOF workflow)
We present a comprehensive analysis of human follicular fluid proteome by comparing several strategies of sample preparation, mass spectrometry analysis, and data processing, resulting in the identification of 1638 proteins. We devised two separate label-free quantitative workflows, termed after the...
ORGANISM(S): Homo sapiens (Human) 
2021-07-23 | PXD024223 | Pride
Lc-ms/ms Oocyte quality control Human follicular fluid Oocyte maturity Total protein approach Swath-ms Proteome Blastocyst development 
ProteomicsAccelerated age-dependent development of endothelial dysfunction in humanized dyslipidemic mice
In the present work endothelial function in the aorta and femoral artery assessed in vivo by magnetic resonance imaging (MRI) was characterized in male and female 8-, 14-, 22-, 28-, and 40-week-old E3L.CETP and C57BL/6J mice. Vascular nitric oxide (NO), eicosanoids and hydrogen peroxide (H2O2) produ...
ORGANISM(S): Mus musculus (Mouse) 
2025-06-30 | PXD029562 | Pride
Qunatitative proteomics Humanized dyslipidemic mice Fasp Endothelial dysfunction Total protein approach 
ProteomicsNitric oxide resistance in Leishmania (Viannia) braziliensis involves upregulation of glucose consumption, glutathione metabolism and increased abundance of proteins with S-nitrosoglutathione reductase activity
In American Tegumentary Leishmaniasis (ATL) production of cytokines, reactive oxygen species (ROS) and nitric oxide (•NO) by host’ macrophages normally lead to parasite death. However, some Leishmania (Viannia) braziliensis strains evade those microbicidal mechanisms, exhibiting a resistance profile...
ORGANISM(S): Leishmania braziliensis 
2022-04-04 | PXD029462 | Pride
Reactive nitrogen species Global proteomics Reactive oxygen species Leishmania Protein concnetrations Nitric oxide resistancy Total protein approach 
ProteomicsQuantitative Characterization of Clinically Relevant Drug-Metabolizing Enzymes and Transporters in Rat Liver and Intestinal Segments for Applications in PBPK Modeling
Rats are extensively used preclinical models for assessing drug pharmacokinetics (PK) and tissue distribution; however, a successful translation of the rat data requires information on the differences in drug metabolism and transport mechanisms between rats and humans. To partly fill this knowledge ...
ORGANISM(S): Rattus norvegicus (Rat) 
2025-02-05 | PXD039271 | Pride
Pbpk modeling Quantitative proteomics Drug metabolism Total protein approach Digoxin Drug transport Interspecies differences 
ProteomicsProteomics informed deconvolution of 3 cell lines
The project aimed to investigate the possibility to use proteomics data to deconvolute cell line proportions in mixed samples. Samples containing either HEK 293, Caco-2, or A549 cells and mixtures of the three cell lines was analysed using the total protein approach. This was then used for proteomic...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD027282 | Pride
Hek 293 A549 Caco-2 
ProteomicsQuantitative evaluation of FASP and MED FASP protocols
In this work we re-evaluate FASP and the related Multienzyme digestion (MED)-FASP method. We use different types of animal tissue and cultured cells, and test the performance of the method under various conditions. We analyze the protein-to-peptide conversion by assessing the yield of peptides, freq...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2016-04-28 | PXD003733 | Pride
Fasp  med fasp  missing cleavages  reproducibility 
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