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ProteomicsRole of the sarcin-ricin loop of 23S rRNA in biogenesis of the 50S ribosomal subunit
In this work, we show that 23S rRNA lacking the SRL can be efficiently reconstituted into 50S subunits, which exhibit full peptidyl transferase (PT) activity. Using cryo-EM and proteomic methods, we also further characterize affinity-purified SRL particles isolated from cells. Collectively, these da...
ORGANISM(S): Escherichia coli 
2025-05-07 | PXD056260 | Pride
Lc-msms Escherichia coli 50s ribosomal subunit Proteomics 
ProteomicsUnderstanding the role of RbgA in the assembly process of the 50S subunit.
Ribosomes must be formed quickly (to support cellular growth demands) and accurately (to ensure proper translation of the genetic code). The assembly of ribosomes in vivo requires not only the constituent rRNAs and ribosomal proteins (r-proteins), but also assembly factors. Several of these factors ...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2022-01-27 | PXD023644 | Pride
Ribosome assembly  50s subunit  ul6 Rbga  ylqf  cryo-electron microscopy. 
ProteomicsProtein bL38 facilitates incorporation of uL6 during assembly of the 50S subunit in Flavobacterium johnsoniae
Previous studies of the 70S ribosome from Flavobacterium johnsoniae revealed a novel ribosomal protein, bL38, which interacts with uL6 on the 50S subunit. This 5.6 kDa protein is conserved across the Bacteroidia and encoded downstream of bL28 and bL33 in a three-gene operon. Here, we show that bL38 ...
ORGANISM(S): Flavobacterium johnsoniae 
2025-05-06 | PXD050205 | Pride
Ribosomes Lc-ms/ms 
ProteomicsSnapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization
Ribosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of the ribosomal subunits, whose production and modifications are steered and regulated by numerous biogenesis factors. In this study, we analyze unperturbed native prokaryotic ribosome biogenesis by is...
ORGANISM(S): Escherichia coli 
2020-12-23 | PXD021374 | Pride
Lc-msms Bacteria Ribosome Shotgun 
UnknownFunctional domains of the 50S subunit mature late in the assembly process.
Not available
S-EPMC3950693 | biostudies-literature
UnknownChloramphenicol Interferes with 50S Ribosomal Subunit Maturation via Direct and Indirect Mechanisms.
Not available
S-EPMC11505724 | biostudies-literature
UnknownYsxC is a placeholder for ribosomal protein uL2 during 50S ribosomal subunit assembly.
Not available
S-EPMC12560758 | biostudies-literature
UnknownCryo-EM structures of the late-stage assembly intermediates of the bacterial 50S ribosomal subunit.
Not available
S-EPMC3737534 | biostudies-literature
ProteomicsFunctional analysis of BipA in E. coli reveals the plasticity of 50S ribosome assembly
BipA is a conserved translational GTPase that resembles elongation factor EF-G and 30S assembly factor LepA. Recent evidence suggests that BipA functions in 50S subunit assembly, but the precise role of the factor remains unclear. Here, we use stable isotope labeling of amino acids in culture and m...
ORGANISM(S): Escherichia coli 
2021-09-09 | PXD017917 | Pride
Ychf Gtpase Protein synthesis Typa Hflx 
UnknownProtein bL38 facilitates incorporation of uL6 during assembly of the 50S subunit in Flavobacterium johnsoniae.
Not available
S-EPMC11851116 | biostudies-literature
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