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ProteomicsPhosphoproteome enrichment strategy using complementary chemistries of Fe-NTA and TiO2 followed by LC-MS/MS
The global phosphoproteome analysis by liquid chromatography-mass spectrometry (LC-MS) has emerged as an important tool to study cell signalling. However, the coverage of phosphoproteome by LC-MS is limited despite various approaches for phosphoprotein enrichment. Studies in literature have exploite...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD050933 | Pride
Affinity chromatography Phosphoproteins Enrichment Lc-ms/ms 
UnknownEfficacy of glycoprotein enrichment by microscale lectin affinity chromatography.
Not available
S-EPMC3658453 | biostudies-literature
OtherComprehensive and reproducible phosphopeptide enrichment using iron immobilized metal ion affinity chromatography (Fe-IMAC) columns.
Data from ProteomeXchange, PXD ID: PXD001060. File: 00730_A11_P005652_S00_T01_R1.mzml. Published as part of Mol Cell Proteomics. 2015 Jan;14(1):205-15 . From the Abstract: {{i}} ... Current offline enrichment approaches using TiO(2), Ti, and Fe immobilized metal ion affinity chromatography (IMAC) m...
ORGANISM(S): Homo_sapiens_viruses, Human 
GPM32310023168 | GPMDB
Resource Reanalysis 
ProteomicsPhosphoproteome enrichment strategy using complementary chemistries of Fe-NTA and TiO2 followed by LC-MS/MS
The global phosphoproteome analysis by liquid chromatography-mass spectrometry (LC-MS) has emerged as an important tool to study cell signalling. However, the coverage of phosphoproteome by LC-MS is limited despite various approaches for phosphoprotein enrichment. Studies in literature have exploite...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) Cercopithecus aethiops (Green monkey) (Grivet) Pinus radiata (Monterey pine) Drosophila melanogaster (Fruit fly) Caenorhabditis elegans Dictyostelium discoideum (Slime mold) Cicer arietinum (Chickpea) (Garbanzo) Bos taurus (Bovine) Candida albicans (Yeast) Homo sapiens (Human) Brassica oleracea var. botrytis (Cauliflower) Bombyx mori (Silk moth) Brassica napus (Rape) 
2025-10-30 | PXD057032 | Pride
Affinity chromatography Phosphoproteins Enrichment Lc-ms/ms 
UnknownComparing multistep immobilized metal affinity chromatography and multistep TiO2 methods for phosphopeptide enrichment.
Not available
S-EPMC4766865 | biostudies-literature
ProteomicsComparison of affinity enrichment methods for O-GlcNAc proteomics
In this work, by using the lysates of PANC-1 (a pancreatic cancer cell line), we provided a head-to-head comparison of three affinity enrichment methods/materials (i.e., antibody, lectin AANL6, and an OGA mutant) for O-GlcNAc proteomics. The enrichment was performed before or after high-pH HPLC frac...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Saccharomyces Cerevisiae (ncbitaxon:4932) 
2024-04-23 | MSV000094604 | MassIVE
UnknownNegative enrichment of target cells by microfluidic affinity chromatography.
Not available
S-EPMC3199139 | biostudies-literature
ProteomicsPlasma Glycopeptide Enrichment
We have compared three different glycopeptide enrichment techniques (SAX-ERLIC, HILIC, and lectin affinity) and a C18 control to a tryptic digest of proteins from depleted human plasma.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-07-02 | MSV000082565 | MassIVE
ProteomicsSequential Phosphoproteomic Enrichment through Complementary Metal-Directed Immobilized Metal Ion Affinity Chromatography
We developed a tip-based sequential IMAC based on different kinds of metal ion for increasing the coverage of phosphoproteome. This approach not only can be used for singly and multiply phosphopeptides separation but also for acidic, basophilic and Pro-directed phosphopeptides separation.
ORGANISM(S): Homo Sapiens (human) 
2016-09-07 | PXD004903 | JPOST Repository
ProteomicsA Spin-Tip Enrichment Strategy for the Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
Post-translational modifications (PTMs) change protein structures and functions. Methods for the simultaneous enrichment of multiple PTM types can maximize coverage in analyses. We present a protocol using dual-functional Ti(IV)-immobilized metal affinity chromatography followed by mass spectrometry...
ORGANISM(S): Homo sapiens (Human) 
2022-08-12 | PXD033065 | Pride
Post-translational modifications (ptms) Phosphopeptides Pancreas N-glycopeptides Dual-functional ti(iv)-immobilized metal affinity chromatography (imac) Electrostatic repulsion hydrophilic interaction chromatography (erlic) Simultaneous enrichment 
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