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ProteomicsA standardized and reproducible workflow for membrane glass slides in routine histology and spatial proteomics
Defining the molecular phenotype of single cells in-situ is essential for understanding tissue heterogeneity in health and disease. Powerful imaging technologies have recently been joined by spatial omics technologies, promising unparalleled insights into the molecular landscape of biological sample...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD040281 | Pride
Antigen retrieval Spatial proteomics Deep visual proteomics Glycerol Membrane slides Histology Proteomics Heat-induced epitope retrieval Laser microdissection 
UnknownFormaldehyde scavengers function as novel antigen retrieval agents.
Not available
S-EPMC4661444 | biostudies-literature
UnknownAntigen retrieval by citrate solution improves western blot signal.
Not available
S-EPMC6423990 | biostudies-literature
UnknownHeat-induced antigen retrieval by using Tris-EDTA solution destroys nuclear structure in certain tissues.
Not available
S-EPMC12816847 | biostudies-literature
GenomicsRole of PARP1 in cocaine-related memory retrieval
Reward-related memory is an important factor in cocaine seeking. One necessary signaling mechanism for long-term memory formation is the activation of poly(ADP-ribose) polymerase-1 (PARP-1), via poly(ADP-ribosyl)ation. We demonstrate herein that auto-poly(ADP-ribosyl)ation of activated PARP-1 was si...
ORGANISM(S): Rattus norvegicus 
2016-06-25 | E-GEOD-83718 | biostudies-arrayexpress
UnknownEffect of Antigen Retrieval on Genomic DNA From Immunodissected Samples.
Not available
S-EPMC9527476 | biostudies-literature
UnknownImpact of antigen retrieval protocols on the immunohistochemical detection of epigenetic DNA modifications.
Not available
S-EPMC10247850 | biostudies-literature
ProteomicsHLA class I peptide analysis from melanoma cell lines
Three melanoma cell lines were cultured and isolated for total HLA class I antigens. Peptides were analysed by LC-MS/MS to profile the antigen repertoire.
ORGANISM(S): Homo sapiens (Human) 
2024-06-11 | PXD036277 | Pride
Immuno-peptidomics Melanoma Affinity mass spectrometry Antigen discovery 
ProteomicsSystematic Evaluation and Optimization of Protein Extraction Parameters in Diagnostic FFPE Specimens
Objectives: Formalin-fixed paraffin-embedded (FFPE) tissue is the standard material for di-agnostic pathology but poses relevant hurdles to accurate protein extraction due to cross-linking and chemical alterations. While numerous extraction pro-tocols and chemicals have been described, systematic c...
ORGANISM(S): Homo sapiens (Human) 
2022-05-19 | PXD029133 | Pride
Protein extraction  formalin-fixed paraffin-embedded tissue  clinical prote-omics  antigen retrieval  crosslinking reversal  protein analysis  biomarker 
UnknownEnhanced antigen retrieval of amyloid β immunohistochemistry: re-evaluation of amyloid β pathology in Alzheimer disease and its mouse model.
Not available
S-EPMC3524564 | biostudies-literature
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