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TranscriptomicsGenomic Library Enrichment for n-Butanol tolerance in E. coli. Samples vs Reference
Genomic Library Enrichment to determine the n-Butanol Tolerant related genes in E. coli. The samples involves a series of batch transfers to increasing concentrations of n-butanol and controls (always grew in absence of the solvent) Two technical replicas. Samples 1, 3, 5, and 7 are the serial tra...
ORGANISM(S): Escherichia coli 
2012-09-19 | E-GEOD-26223 | biostudies-arrayexpress
TranscriptomicsA transcriptional study of acidogenic chemostat cells of Clostridium acetobutylicum - cellular behavior in adaptation to n-butanol
Clostridium acetobutylicum is well-known for its butanol production. Butanol toxicity is a major drawback for the generation of high-butanol producing strains. Here, the transcriptional response a steady state, acidogenic (pH 6), phosphate-limited Clostridium acetobutylicum chemostat culture to diff...
ORGANISM(S): Clostridium acetobutylicum 
2012-01-30 | E-MEXP-3571 | biostudies-arrayexpress
TranscriptomicsTranscriptional Response of the thermoacidophilic Archaeon Sulfolobus acidocaldarius to solvent stress exemplified by 1-butanol exposure
To study the cellular global response of the thermoacidophilic Archaeon Sulfolobus acidocaldarius upon solvent exposure we performed transcriptome comparing the response of planktonic and biofilm cells in absence and presence 1-butanol. S. acidocaldarius was grown in Petri dishes (static incubation...
ORGANISM(S): Sulfolobus acidocaldarius DSM 639 
2021-02-20 | E-MTAB-10093 | biostudies-arrayexpress
TranscriptomicsTranscriptome analysis of Escherichia coli C strains (wt and PKH5000, a butanol-tolerant mutant) after 1-butanol treatment
The goal of this study is to explore genes that are differentially expressed in E. coli C strains (wt and a butanol-tolerant mutant) after 1-butanol treatment. The butanol-tolerant mutant strain PKH5000 (denoted by 'E' for 'evolved') were derived from KCTC 2571 (wt) (denoted by 'A' for 'ancestral') ...
ORGANISM(S): Escherichia coli 
2013-07-01 | E-GEOD-47589 | biostudies-arrayexpress
ProteomicsClostridium cellulovorans proteomic responses to butanol stress
Combination of butanol-hyperproducing and hypertolerant phenotypes is essential for developing microbial strains suitable for industrial production of bio-butanol, i.e. one of most promising liquid biofuels. Clostridium cellulovorans is among the microbial strains with the highest potential for dire...
ORGANISM(S): Clostridium cellulovorans 
2021-08-13 | PXD024183 | Pride
Atpase Rex Stringent response Hfq Heat shock proteins 
TranscriptomicsTranscriptome analysis of isolated Escherichia coli mutants using VERT for the study of n-butanol tolerance
Transcriptome analysis of isolated mutants using the method Visualizing Evolution in Real-Time (VERT) for the study of n-butanol tolerance. The samples were isolated from an evolution experiment picking samples at different times based in the evolution dynamics obtained with VERT. Mutants were gro...
ORGANISM(S): Escherichia coli K-12 
2012-03-30 | E-GEOD-30005 | biostudies-arrayexpress
TranscriptomicsFunctional Genomic Study of Exogenous n-Butanol Stress in Escherichia coli
n-Butanol has been proposed as an alternative biofuel to ethanol, and both Escherichia coli and Saccharomyces cerevisiae have been engineered to produce it. Unfortunately, n-butanol is more toxic than ethanol to these organisms. To understand the basis for its toxicity, cell wide studies were cond...
ORGANISM(S): Escherichia coli 
2010-05-16 | E-GEOD-16973 | biostudies-arrayexpress
TranscriptomicsButanol Challenge of L. brevis
The presence of anti-microbial phenolic compounds, such as the model compound ferulic acid, in biomass hydrolysates poses significant challenges to the widespread use of biomass in conjunction with whole cell biocatalysis or fermentation. Biofuel toxicity must also be overcome to allow for efficient...
ORGANISM(S): Lactobacillus brevis 
2011-12-31 | E-GEOD-24944 | biostudies-arrayexpress
TranscriptomicsCcpA, a Pleiotropic Key Regulator in Butanol-producing Clostridium acetobutylicum ATCC 824
Clostridium acetobutylicum is a typical bacterium of major importance to industrial butanol production. In order to dissect the regulatory network pertaining to the industrial application of this bacterium, catabolite control protein A (CcpA) was investigated for its global function by DNA microarra...
ORGANISM(S): Clostridium acetobutylicum 
2012-08-30 | E-GEOD-33364 | biostudies-arrayexpress
TranscriptomicsMetabolite stress response to butanol in Clostridium acetobutylicum ATCC 824
Clostridium acetobutylicum is a Gram positive, endospore forming firmicute that has been known as the model organims for ABE (acetone-butanol-ethanol) fermentation. With its ability to consume a wide variety of substrates, C. acetobutylicum carries out a biphasic ABE fermentation, which consists of ...
ORGANISM(S): Clostridium acetobutylicum ATCC 824 
2013-08-20 | E-GEOD-48031 | biostudies-arrayexpress
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