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ProteomicsMiniaturized sample preparation on a digital microfluidics device for sensitive bottom-up microproteomics of mammalian cells using magnetic beads and mass spectrometry-compatible surfactants
We used digital microfluidics (DMF) for the sample preparation of approximately 100 mammalian cells, and subsequent bottom-up proteome analysis by LC-MS. This comprised optimization of cell lysis conditions for DMF, the development of detergent-buffer systems, and adaptation of the single-pot, solid...
ORGANISM(S): Homo sapiens (Human) 
2019-10-07 | PXD014158 | Pride
Digital microfluidics Detergents Sp3 Microproteomics Miniaturization 
ProteomicsCombination of digital microfluidics and magnetic bead-based intact proteoform elution for top-down nanoproteomics of single C. elegans
While most nanoproteomics approaches for the analysis of low-input samples are based on bottom-up LC-MS workflows, top-down approaches enabling proteoform characterization are still underrepresented. Using mammalian cell proteomes, we here established a facile one-pot sample preparation protocol bas...
ORGANISM(S): Caenorhabditis elegans 
2023-06-19 | PXD039883 | Pride
Top-down proteomics  nanoproteomics  caenorhabditis elegans  digital microfluidics 
GenomicsDigital hydrogel microfluidics for high-throughput single-cell sequencing with split and pool barcoding
Hydrogel and digital microfluidics assisted high-throughput single-cell sequencing technology
PRJEB101113 | ENA
ProteomicsIsobaric peptide labeling on digital microfluidics for quantitative proteomics of low cell numbers is facilitated by low critical micellar concentration detergents
We developed protocols for isobaric TMT labeling on digital microfluidics (DMF) devices enabling the quantitative proteome analysis of approximately 25 mammalian cells per channel by subsequent bottom-up proteome analysis by LC-MS. This comprised identification of a compatible detergent for digestio...
ORGANISM(S): Homo sapiens (Human) 
2022-02-28 | PXD023226 | Pride
Digital microfluidics Detergents Cmc Tmt labeling Sp3 
GenomicsDigital-scRRBS: A cost-effective, highly sensitive and automated single-cell methylome analysis platform via digital microfluidics
Digital-scRRBS: A cost-effective, highly sensitive and automated single-cell methylome analysis platform via digital microfluidics
PRJNA883959 | ENA
Methylation profilingDigital-scRRBS: A cost-effective, highly sensitive and automated single-cell methylome analysis platform via digital microfluidics
Single-cell DNA methylation sequencing is a powerful method for elucidating important physiological and pathological processes, identifying cell subpopulations, and constructing epigenetic regulatory networks. Existing methylome analyses typically require substantial starting materials, complex oper...
ORGANISM(S): Homo sapiens 
2022-09-29 | GSE214106 | GEO
ProteomicsAll-in-One Digital Microfluidics Pipeline for Proteomic Sample Preparation and Analysis
Dimethyl labeling was integrated into the microfluidic pipeline to allow for precise relative quantification of the trace samples at the nanogram level, and the new pipeline was applied to evaluating cancer cell lines and cancer tissue samples.
ORGANISM(S): Homo sapiens (Human) 
2023-03-24 | PXD036982 | Pride
Digital microfluidics Proteomics Dimethyl labeling Cancer sub-typing Interface Miniaturized sample 
GenomicsDMF-scMT-seq Linking Methylome and Transcriptome within Single Cells with Digital Microfluidics
DMF-scMT-seq Linking Methylome and Transcriptome within Single Cells with Digital Microfluidics
PRJNA1064572 | ENA
ProteomicsIntegrated Single-Cell Proteomics Analysis on an Active Matrix Digital Microfluidic Chip
We introduce and implement a comprehensive single-cell proteomics sample pre-treatment solution based on an active matrix digital microfluidics chip(AM-DMF-SCP). This platform accommodates high-throughput single-cell isolation and seamless, non-destructive sample pre-treatment, characterized by its ...
ORGANISM(S): Homo Sapiens 
PXD048434 |
GenomicsSimultaneous Single-cell Genome and Transcriptome Sequencing with Digital Microfluidics Identifying Essential Driving Genes
Simultaneous Single-cell Genome and Transcriptome Sequencing with Digital Microfluidics Identifying Essential Driving Genes
PRJNA818193 | ENA
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