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ProteomicsAnti-DnaA antibodies specificity validation by mass spectrometry
The ubiquitous protein DnaA is a chromosomal DNA replication initiator and transcription factor. It binds to 9-bp DNA sites called "DnaA-boxes" that are localized within the replication origin (oriC) and throughout chromosomal DNA, usually in the proximity of the promoter regions. During stress, bac...
ORGANISM(S): Escherichia coli 
2026-03-02 | PXD061973 | Pride
Sample specificity Escherichia coli Chip-seq Dnaa Rabbit antibodies 
TranscriptomicsRegulation of initiation of chromosome replication by oriC-proximal DnaA-box clusters in Bacillus subtilis
Comparison of transcriptions in wild_type, delta-6 (deletion of six DnaA-box clusters (DBCs)), delta-5 and delta-DBC[yydA-yycS] strains, to see effect of the DBCs on transcription of neighboring genes and DnaA-regulated genes.
ORGANISM(S): Bacillus subtilis 
2011-07-01 | E-MEXP-3069 | biostudies-arrayexpress
TranscriptomicsExpression profiles of dnaA null, rok null, and dnaA null; rok null mutants
To identify potential targets of co-regulation by DnaA and Rok, we compared the transcriptional profiles of dnaA null, rok null, and dnaA null rok null mutants. Because a dnaA null mutant requires an oriC- strain background, we used an oriC- oriN+ background for all strains, to allow direct compari...
ORGANISM(S): Bacillus subtilis 
2015-06-13 | E-GEOD-69788 | biostudies-arrayexpress
TranscriptomicsGenome-wide binding of replication initiation proteins in Bacillus subtilis
Initiation of DNA replication requires binding of the initiator protein, DnaA, to specific binding sites in the chromosomal origin of replication, oriC. In low G+C Gram-positive bacteria, the primosomal proteins DnaD and DnaB, in conjunction with loader ATPase DnaI, load the replicative helicase at ...
ORGANISM(S): Bacillus subtilis 
2010-12-07 | E-GEOD-23686 | biostudies-arrayexpress
ProteomicsThe Escherichia coli replication initiator DnaA is titrated on the chromosome
DNA replication initiation is orchestrated in bacteria by the replication initiator DnaA. Two models for regulation of DnaA activity in Escherichia coli have been proposed: the switch between an active and inactive form and the titration of DnaA on the chromosome. Although proposed decades ago, expe...
ORGANISM(S): Escherichia coli 
2025-07-28 | PXD064841 | Pride
Titration model Single-particle tracking photoactivatable localisation microscopy Switch model Dnaa protein Oric Dnaa boxes chromosome binding Data Replication initiator 
ProteomicsStructures reveal DnaA domain I dimer conserved across Actinomycetes: implications for replication initiation
DNA replication is a fundamental process in biology with initiation marking its key, first step. In bacteria, DNA replication is initiated by the DnaA protein. DnaA exhibits multidomain architecture, consisting of an N-terminal domain I, linker region, AAA+ family ATPase cassette, and C-terminal DN...
ORGANISM(S): Homo sapiens (Human) 
2026-05-27 | PXD074705 | Pride
Hdx ms  dnaa  dimerization  dna replication  dimeric interface  hydrogen/deuterium exchange mass spectrometry 
ProteomicsEstablishment of titration-based control of DNA replication in Escherichia coli
Escherichia coli couples the initiation of DNA replication with cell size by modulating the activity of the replication initiator protein DnaA. The activity of DnaA is regulated both by its interconversion between an active and inactive form and by its titration on chromosomal binding sites. Whereas...
ORGANISM(S): Escherichia coli 
2026-09-10 | PXD075568 | Pride
Hyper-replicative stress Dnaa-atp/dnaa-adp switch Replication forks Dnaa boxes Dnaa Titration-based control of dna replication 
TranscriptomicsCGH of S. meliloti DnaA and Hda overproducing strains
Assays 1-4: Effects of DnaA activity on S. meliloti chromosome, pSymA and pSymB replicon bias. Sheared DNA extracts of IPTG-induced vs uninduced cultures of DnaA (Assays 1,2) or Hda (Assays 3,4) overexpressors were labeled with Cy3 or Cy5 and hybridized applying dye-swap replicate design. Assay 5: H...
ORGANISM(S): Sinorhizobium meliloti 
2015-10-04 | E-MTAB-3924 | biostudies-arrayexpress
TranscriptomicsExpression profiles of dnaA null, rok null, and dnaA null; rok null mutants
To identify potential targets of co-regulation by DnaA and Rok, we compared the transcriptional profiles of dnaA null, rok null, and dnaA null rok null mutants. Because a dnaA null mutant requires an oriC- strain background, we used an oriC- oriN+ background for all strains, to allow direct comparis...
ORGANISM(S): Escherichia coli Bacillus subtilis subsp. subtilis str. 168 Bacillus subtilis 
2015-06-13 | GSE69788 | GEO
GenomicsChIP-Seq DnaA-SPA and AcpS-SPA control
ChIP-Seq DnaA-SPA and AcpS-SPA control
PRJEB56374 | ENA
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