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ProteomicsIdentification of ChIP-seq and RIME grade antibodies for Estrogen Receptor alpha
Estrogen Receptor alpha (ERα) is a key driver of most breast cancers, and it is the target of endocrine therapies used in the clinic to treat women with ERα positive (ER+) breast cancer. The two methods ChIP-seq (chromatin immunoprecipitation coupled with deep sequencing) and RIME (Rapid Immunopreci...
ORGANISM(S): Homo sapiens (Human) 
2019-03-21 | PXD012930 | Pride
Rime Estrogen receptor alpha Chip-seq 
GenomicsComparing genome-wide chromatin profiles using ChIP-chip or ChIP-seq
The goal of the ChIP-seq study was to investigate the distribution of the TATA-binding protein (TBP) across the human genome. TBP is the DNA-binding subunit of the basal transcription factor TFIID for RNA polymerase II (pol II) and it also participates in other complexes for the other RNA polymerase...
ORGANISM(S): Homo sapiens 
2010-03-02 | E-GEOD-17937 | biostudies-arrayexpress
GenomicsGenome-wide ChIP-seq analysis of Pou3f1 during mouse pluripotent stem cell neural fate commitment
Appropriate neural initiation of the pluripotent stem cells in the early embryos is critical for the development of the central nervous system. This process is regulated by the coordination of extrinsic signals and intrinsic programs. However, how the coordination is achieved to ensure proper neural...
ORGANISM(S): Mus musculus 
2015-11-23 | E-GEOD-69778 | biostudies-arrayexpress
GenomicsGenome-wide ChIP-Seq reveals a dramatic shift in the binding of the transcription factor erythroid Kruppel-like factor during erythrocyte differentiation
Erythropoiesis is dependent on the activity of transcription factors, including the erythroid-specific erythroid Kruppel-like factor (EKLF). ChIP followed by massively parallel sequencing (ChIP-Seq) is a powerful, unbiased method to map transfactor occupancy. We used ChIP-Seq to study the interactom...
ORGANISM(S): Mus musculus 
2013-06-17 | E-GEOD-48020 | biostudies-arrayexpress
GenomicsGenome-wide identification of binding sites for NAC and YABBY transcription factors and co-regulated genes during soybean seedling development by ChIP-Seq and RNA-Seq.
In order to dissect the gene regulatory network during the functional transition of cotyledons from non-photosynthetic storage tissue to metabolically active photosynthetic tissue, we constructed ChIP-Seq libraries for NAC and YABBY transcription factors using pooled cotyledons from seedling develop...
ORGANISM(S): Glycine max 
2013-08-14 | E-GEOD-42422 | biostudies-arrayexpress
GenomicsDetecting differential peaks in ChIP-seq signals with ODIN
Motivation: Detection of changes in DNA-protein interactions from ChIP-seq data is a crucial step in unraveling the regulatory networks behind biological processes. The simplest variation of this problem is the differential peak calling problem. Here one has to find genomic regions with ChIP-seq sig...
ORGANISM(S): Mus musculus 
2015-01-07 | E-GEOD-57563 | biostudies-arrayexpress
GenomicsDouble fragmentation ChIP-seq provides single nucleotide resolution Tcf4 binding characteristics
ChIP-seq has become the method of choice for studying functional DNA-protein interactions on a genome wide scale. The method is based on co-immunoprecipitation of DNA binding proteins with formaldehyde cross-linked DNA, followed by deep sequencing of immunoprecipitated chromatin fragments, allowing ...
ORGANISM(S): Homo sapiens 
2010-11-01 | E-GEOD-18481 | biostudies-arrayexpress
GenomicsH3K27Ac and CENPA ChIP-seq experiments for human TeloHAEC cells
This data contributes to a telomere-to-telomere genome assembly and annotation of the naked mole-rat, Heterocephalus glaber. To epigenetically annotate centromeres, we performed CENPA ChIP-seq experiments in the male subordinate NMR livers. To ensure antibody quality, as this was the first time CENP...
ORGANISM(S): Homo sapiens 
2026-08-05 | E-MTAB-17311 | biostudies-arrayexpress
GenomicsH3K27Ac and CENPA ChIP-seq experiments for the naked mole-rat liver
This data contributes to a telomere-to-telomere genome assembly and annotation of the naked mole-rat, Heterocephalus glaber. To epigenetically annotate centromeres, we performed CENPA ChIP-seq experiments in the male subordinate NMR livers. These experiments included an Input control, and a H3K27Ac ...
ORGANISM(S): Heterocephalus glaber 
2026-08-05 | E-MTAB-17296 | biostudies-arrayexpress
GenomicsChIP-seq profiling of histone modifications (H3K4me3, H3K9ac, and H3K27me3) in seedling shoot tissues from 10 barley genotypes
ChIP-seq was performed to characterize histone modification landscapes and to enable comparative epigenomic analyses across the barley pangenome. Leaf tissues from seedling shoots of 10 barley genotypes were collected from plants grown under controlled conditions (16 h light/8 h dark, 20°C day/16°C ...
ORGANISM(S): Hordeum vulgare subsp. spontaneum 
2026-07-12 | E-MTAB-17344 | biostudies-arrayexpress
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