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TranscriptomicsIdentification of FUS RNA targets in HeLa cells
We identified a landscape of FUS-binding RNA targets in HeLa cells. The majority of the FUS binding sites are in introns of pre-mRNAs and less are in exons and untranslated regions. Significant FUS binding in introns flanking cassette exons, long intron (>100kb) containing transcripts and noncoding...
ORGANISM(S): Homo sapiens 
2013-12-16 | E-GEOD-50178 | biostudies-arrayexpress
TranscriptomicsFUS KO mRNA sequencing and anti-FUS RNA immunoprecipitation sequencing
The aim of our study is to identify the role of FUS in shaping the transcriptome. RNA-seq of two FUS KO clones was performed and compared to wt; for each, four replicates were sequenced. RNA molecules associated with the FUS protein were determined by means of a RNA immuno-precipitation, followed by...
ORGANISM(S): Homo sapiens 
2016-06-13 | E-GEOD-71812 | biostudies-arrayexpress
TranscriptomicsIdentification of in vivo, conserved, FUS RNA binding sites and the impact of FUS on the neuronal transcriptome
FUS, an RNA binding protein was recently implicated in Amyotrophic Lateral Sclerosis (ALS). ALS is a fatal neurodegenerative disease. We report the identification of the conserved neuronal RNA targets of FUS and the assessment of the impact of FUS depletion on the neuronal transcriptome. We identif...
ORGANISM(S): Homo sapiens 
2013-01-25 | E-GEOD-43308 | biostudies-arrayexpress
GenomicsHITS-CLIP analysis of FUS in lineage-committed cells.
In the present study, we performed HITS-CLIP analysis for FUS using mouse brain to extensively characterize tits RNA-binding sites and functional roles in RNA metabolisms. We identified preferential binding of FUS to stem-and-loop structures but without any discernible consensus motifs. FUS was pref...
ORGANISM(S): Mus musculus 
2012-07-26 | E-GEOD-37190 | biostudies-arrayexpress
TranscriptomicsExpression profiles of primary cortical neurons after knocking down Fus
To clarify the functional properties of FUS, we established the differentially expressed alternative exons in FUS-silenced primary cortical neurons by using exon-sensitive microarray technology. We analyzed total RNA of primary cortical neuron infected with lentivirus expressing shRNA against mouse ...
ORGANISM(S): Mus musculus 
2012-07-23 | E-GEOD-36153 | biostudies-arrayexpress
TranscriptomicsToxic gain of function from mutant FUS protein is crucial to trigger cell autonomous motor neuron loss
FUS is an RNA-binding protein involved in amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Cytoplasmic FUS-containing aggregates are often associated with concomitant loss of nuclear FUS. Whether loss of nuclear FUS function, gain of a cytoplasmic function, or a combination of ...
ORGANISM(S): Mus musculus 
2016-03-14 | E-GEOD-78730 | biostudies-arrayexpress
ProteomicsCharacterization of genetic loss-of-function of Fus in zebrafish
The RNA-binding protein FUS is implicated in transcription, alternative splicing of neuronal genes and DNA repair. Mutations in FUS have been linked to human neurodegenerative diseases such as ALS (amyotrophic lateral sclerosis). We genetically disrupted fus in zebrafish (Danio rerio) using the CRIS...
ORGANISM(S): Danio rerio (Zebrafish) (Brachydanio rerio) 
2018-06-15 | PXD004876 | Pride
Crispr Zebrafish Fus protein 
TranscriptomicsExpression signatures of human HeLa cells with RNAi targetting A1 hnRNP, FUS, H hnRNP and p68-DDX5
Transcription profiling of human HeLa cells (cervical cancer cell line) transfected with a plasmid expressing shRNAs cloned into the pSuper expression vector compared to emprty vector negative controls for transfection. Four different RNA interference treatments targetted: A1 hnRNP (HNRNPA1, Heterog...
ORGANISM(S): Homo sapiens 
2008-08-20 | E-GEOD-12058 | biostudies-arrayexpress
ProteomicsHspB8 controls hardening and molecular aging of FUS condensates by domain-specific protein interactions Part 1
Biomolecular condensates formed by phase separation offer a confined space where protein-protein interactions (PPIs) facilitate distinct biochemical reactions. As their aberrant behavior is increasingly associated with disease, it is crucial to understand the molecular organization of PPIs within co...
ORGANISM(S): Homo sapiens (Human) 
2021-10-12 | PXD021114 | Pride
Phase separation Molecular aging Qxl-ms Fus Hspb8 Hsp27 
TranscriptomicsMutant FUS triggers age-dependent synaptic impairment in presymptomatic ALS-FUS mice (CLIP-seq)
FUS is a primarily nuclear RNA-binding protein with important roles in RNA processing and transport. FUS mutations disrupting its nuclear localization characterize a subset of amyotrophic lateral sclerosis (ALS-FUS) patients, through an unidentified pathological mechanism. FUS regulates nuclear RNA,...
ORGANISM(S): Mus musculus 
2021-03-05 | E-MTAB-9211 | biostudies-arrayexpress
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