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ProteomicsIntensity-based absolute quantification (iBAQ) of proteins in MDA-MB231
To determine the absolute copy number of proteins in MDA-MB231 breast cancer cells, we employed IBAQ mediated absolute quantification of proteins based on (Schwanhäusser et al., Nature, 2011), with some modifications. Maqquant calculated iBAQ values were calibrated using spike-in standards, and used...
ORGANISM(S): Homo sapiens (Human) 
2021-05-14 | PXD024139 | Pride
Ibaq  absolute protein quantification  breast cancer  mda-mb231 
UnknownThe Lottia gigantea shell matrix proteome: re-analysis including MaxQuant iBAQ quantitation and phosphoproteome analysis.
Not available
S-EPMC4094399 | biostudies-literature
ProteomicsiBAQ Quantification of the Caulerpa prolifera Proteome
MaxQuant 2.1.3.0 iBAQ analysis of triplicate Caulerpa prolifera samples. Whole algae samples were frozen, ground, extracted with acetone and phenol, recovered by precipitation, and solubilized for SDS-PAGE. For each of the triplicate samples the gel lane was cut into 12 gel slices and prepared by ...
ORGANISM(S): Caulerpa Prolifera (ncbitaxon:76314) 
2023-03-08 | MSV000091436 | MassIVE
ProteomicsIntensity-based absolute quantification (iBAQ) of components of photosystem I monomers and dimers.
Photosystem I (PSI) enables photo-electron transfer and regulates photosynthesis in the bioenergetic membranes of cyanobacteria and chloroplasts. Being a multi-subunit complex, its macromolecular organization affects the dynamics of photosynthetic membranes. Here we reveal a chloroplast PSI from the...
ORGANISM(S): Chlamydomonas reinhardtii 
2023-03-11 | PXD026990 | Pride
Photosystem i Psi Ibaq Chlamydomonas reinhardtii 
ProteomicsYeast cell cycle proteome and phosphoproteome (LC-MSMS)
Multi-omic absolute quantitative analysis of the yeast (Saccharomyces cerevisiae) mitotic cell cycle. Transcriptomics (RNA-Seq), proteomics (SILAC/ iBAQ), phosphoproteomics (SILAC/ iBAQ, enrichment with TiO2), and untargeted metabolomics (Metabolon, Inc.) were performed, all in biological triplicate...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-03-26 | PXD016519 | Pride
Lc-msms Budding yeast Multi-omics Mitosis Respiration Fermentation Ibaq Cell cycle Silca 
ProteomicsProteomic Analysis of fly brain cytoplasmic RNP granules containing the lncRNA mimi
In neurons, mRNAs and associated RNA-binding proteins assemble into ribonucleoprotein (RNP) granules essential to regulate mRNA trafficking, local translation, and turnover. Dysregulation of RNA-protein condensation can disturb synaptic plasticity. We report that the novel lncRNA mimi is a constitut...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2022-10-12 | PXD034453 | Pride
Rnp granules  fly brain  neurons  lncrna mimi Fne  rbp9 Drosophila  relative ibaq  quantitative proteomics 
ProteomicsProteomic Analysis of Loricrin Knockout Mouse Epidermis IBAQ
The cross-linked envelope of the mammalian epidermal corneocyte is important as a scaffold for assembly of the lipid barrier of the epidermis. Illustrating its importance, deficient envelope cross-linking by keratinocyte transglutaminase (TGM1) is a major cause of the autosomal recessive congenital ...
ORGANISM(S): Mus musculus (Mouse) 
2016-12-14 | PXD003532 | Pride
Loricrin Mouse Epidermis 
ProteomicsDon’t Waste Time – Ensure Success in Your Cross-Linking Mass Spectrometry Experiments Before You Begin.
Cross-linking mass spectrometry (XL-MS) is becoming a more popular tool for researchers to turn towards for studying proteins and their complexes of interest especially in complex samples such as lysates or whole-cells. Studying a targeted proteins in a complex mixture can be difficult as data on ot...
ORGANISM(S): Neisseria meningitidis serogroup C (strain 8013) Bacteria 
2024-02-07 | PXD045792 | Pride
Cross-linking Mass spectrometry Protein abundance Ibaq 
ProteomicsComparison and applications of label-free absolute proteome quantification methods on Escherichia coli
Three different label-free proteome quantification methods - APEX, emPAI and iBAQ - were evaluated to measure proteome-wide protein concentrations in the cell. All the methods were applied to a sample from Escherichia coli chemostat culture. A Pearson squared correlation of approximately 0.6 among t...
ORGANISM(S): Escherichia coli 
2013-07-24 | PXD000283 | Pride
Ups2 Shotgun proteomics Ibaq E. coli 
ProteomicsQuantitative analysis of protein ubiquitination
Protein ubiquitination orchestrates nearly all eukaryotic cellular events.1 It starts by attaching ubiquitin through isopeptide bonds to a single or multiple lysine residues of a target protein via a coordinated enzymatic reaction involving activating (E1), conjugating (E2), and ligating (E3) enzyme...
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD037416 | Pride
Ibaq-ub Ubiquitination Stoichiometry Lcms Quantitative proteomics 
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