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TranscriptomicsKnockdown of mitochondrial MnSOD by inducible RNAi in Arabidopsis leaves
Mitochondrial antioxidant defence was manipulated by dexamthasone inducible RNAi knockdown of the mitochondrial superoxide dismutase (MSD1) to study transcriptional changes in response.
ORGANISM(S): Arabidopsis thaliana 
2011-11-01 | E-TABM-1197 | biostudies-arrayexpress
ProteomicsTime-resolved inducible knock-down of Clp protease subunits in tobacco identifies putative protease substrates
The Clp protease complex in plants is the one with the highest complexity through all life kingdoms. It is composed of 14 nuclear- and plastid-encoded subunits which are allocated in different rings across the complex. The proteolytic ring (P-ring; ClpP3-ClpP6) and non-proteolytic ring (R-ring; ClpR...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-02-22 | PXD008305 | Pride
Clp protease  chloroplast  protein degradation  protease substrate  nicotiana tabacum  inducible rnai  lc-msms  shotgun proteomics 
TranscriptomicsThe RDE-10/RDE-11 complex triggers RNAi induced mRNA degradation by association with target mRNA in C. elegans
The molecular mechanisms for target mRNA degradation in C. elegans undergoing RNA interference (RNAi) are not fully understood. Using a combination of genetic, proteomic and biochemical approaches, we report a divergent RDE-10/RDE-11 complex that is required for RNAi in C. elegans. The RDE-10/RDE-...
ORGANISM(S): Caenorhabditis elegans 
2012-04-25 | E-GEOD-36494 | biostudies-arrayexpress
TranscriptomicsRNAi triggered by specialized machinery silences developmental genes and retrotransposons (ChIP-chip)
RNAi is a conserved mechanism in which small interfering RNAs (siRNAs) guide the degradation of cognate RNAs, but also promote heterochromatin assembly at repetitive DNA elements such as centromeric repeats. However, the full extent of RNAi functions and its endogenous targets have not been explored...
ORGANISM(S): Schizosaccharomyces pombe 
2012-11-26 | E-GEOD-41642 | biostudies-arrayexpress
TranscriptomicsRNA expression for dcr1D, rdp1D, ago1D and swi6D
RNA interference (RNAi) pathways are prevalent throughout the eukaryotic kingdom and well known to regulate gene expression on a post-transcriptional level in the cytoplasm. Less is known about possible functions of RNAi in the nucleus. In the fission yeast Schizosaccharomyces pombe, RNAi is crucial...
ORGANISM(S): Schizosaccharomyces pombe 
2012-03-19 | E-GEOD-36113 | biostudies-arrayexpress
TranscriptomicsA genome-scale shRNA resource for transgenic RNAi in Drosophila
Existing transgenic RNAi resources in Drosophila utilize the expression of long double stranded hairpin RNAs and are powerful tools for the identification and characterization of genes involved in various processes. However, they are ineffective to knockdown genes during oogenesis, an important mode...
ORGANISM(S): Drosophila melanogaster 
2011-03-26 | E-GEOD-27039 | biostudies-arrayexpress
TranscriptomicsHigh throughput decoding of anti trypanosomal drug efficacy and resistance
We used all five current human African trypanosomiasis drugs for genome-scale RNA interference (RNAi) target sequencing (RIT-seq) screens in Trypanosoma brucei, revealing the transporters, organelles, enzymes and metabolic pathways that function to facilitate anti-trypanosomal drug action. RIT-seq p...
ORGANISM(S): Trypanosoma brucei 
2011-11-29 | E-ERAD-30 | biostudies-arrayexpress
ProteomicsCharting Organellar Importomes by Quantitative Mass Spectrometry – PEX14c
Protein import into organelles is essential for all eukaryotes and facilitated by multi-protein translocation machineries. Analyzing whether a protein is transported into an organelle is largely restricted to single constituents. This renders knowledge about imported proteins incomplete, limiting ou...
ORGANISM(S): Trypanosoma brucei 
2017-06-14 | PXD004972 | Pride
Trypanosoma brucei Proteomics Glycosomes Organellar protein import Mitochondria Silac Importomics Rnai-mediated protein knockdown 
ProteomicsAn Msp1-containing complex removes orphaned proteins in the mitochondrial outer membrane of trypanosomes (RNAi knockdown data)
The AAA-ATPase Msp1 extracts mislocalized outer membrane proteins and thus contributes to mitochondrial proteostasis. Using pull down experiments, we show that trypanosomal Msp1 localizes to both the glycosome and the mitochondrial outer membrane, where it forms a stable complex with four outer memb...
ORGANISM(S): Trypanosoma brucei 
2023-07-26 | PXD039634 | Pride
Trypanosoma brucei Proteomics Peptide stable isotope dimethyl labeling Mitochondrial quality control Rnai-mediated protein knockdown 
GenomicsRNAi targeting HnRNPH in Tbrucei
RNAi targeting HnRNPH in Tbrucei
PRJEB50037 | ENA
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