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MetabolomicsTrapped ion mobilty improves annotation accuracy in LC-HRMS screening applications for exposomics

The widespread use of a large number of chemicals results in a significant exposure of humans and the environment, posing potential risks to human health and ecosystems. To assess the exposure to chemical mixtures, screening applications covering a large number of analytes based on liquid chromat...

2025-10-14 | MTBLS11753 | MetaboLights
ProteomicsA Triple Knockout (TKO) Proteomics Standard for Diagnosing Ion Interference in Isobaric Labeling Experiments
Isobaric labeling is a powerful strategy for quantitative mass spectrometry-based proteomic investigations. A complication of such analyses has been the co-isolation of multiple analytes of similar mass-to-charge resulting in the distortion of relative protein abundance measurements across samples. ...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2019-08-23 | MSV000084230 | MassIVE
ProteomicsA causal model of ion interference enables assessment and correction of ratio compression in multiplex proteomics
We thoroughly characterize isobaric labeling (TMT)-associated reporter ion interference at the MS2 level using a defined two-proteome experimental system with known ground truth. We discover remarkably poor agreement between the apparent precursor purity in the isolation window and the actual level ...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae Saccharomyces cerevisiae (Baker's yeast) 
2023-12-19 | PXD040449 | Pride
Ion interference Tmt Ratio compression Isobaric label 
ProteomicsA Triple Knockout (TKO) Proteomics Standard for Diagnosing Ion Interference in Isobaric Labeling Experiments
Isobaric labeling is a powerful strategy for quantitative mass spectrometry-based proteomic investigations. A complication of such analyses has been the co-isolation of multiple analytes of similar mass-to-charge resulting in the distortion of relative protein abundance measurements across samples. ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2017-10-26 | PXD008009 | Pride
Ion interference Ms standard Tmt Muiltinotch Lumos Orbitrap fusion 
ProteomicsTKO6: A Peptide Standard to Assess Interference for Unit-Resolved Isobaric Labeling Platforms
Protein abundance profiling using isobaric labeling is a well-established quantitative mass spectrometry technique. However, ratio distortion resulting from co-isolated and co-fragmented ions - commonly referred to as interference - remains a caveat of this technique. Tribrid mass spectrometers, suc...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-03-07 | PXD011653 | Pride
Tko  interference  tmt  itraq  sps-ms3 
ProteomicsExtensive Peptide Fractionation and y1 Ion-based Interference Detection Enable Accurate Quantification by Isobaric Labeling and Mass Spectrometry
Isobaric labeling quantification by mass spectrometry (MS) has emerged as a powerful technology for multiplexed large-scale protein profiling, but measurement accuracy in complex mixtures is confounded by the interference from co-isolated ions, resulting in ratio compression. Here we report that the...
ORGANISM(S): Escherichia coli 
2022-02-28 | PXD005797 | Pride
Isobaric labeling Proteomics Tmt Mass spectrometry And ratio compression Itraq Lc-ms/ms 
ProteomicsComplement Reporter Ion Quantification with a Quadrupole-Ion Trap Instrument
Proteomics workflows have traditionally been divided into discovery-based and targeted approaches, with instrumentation optimized for each. Discovery experiments typically utilize high-resolution analyzers such as the Orbitrap™, while targeted workflows rely on the sensitivity and specificity of tri...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) Xenopus laevis (African clawed frog) 
2025-09-08 | PXD062540 | Pride
Stellar Complement reporter ion Quadrupole-ion trap Multiplexed shotgun proteomics 
ProteomicsAssessing Interference in Isobaric Tag-based Sample Multiplexing using an 18-plex Interference Standard
Reporter ion interference remains a limitation of isobaric tag-based sample multiplexing. Advances in instrumentation and data acquisition modes, such as the recently developed real-time database search (RTS), can reduce interference. However, interference persists as does the need to benchmark upst...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-01-06 | PXD029458 | Pride
Faims Eclipse Ifi Sps-ms3 Rts Tmtpro18 
ProteomicsA Triple Knockout (TKO) Proteomics Standard for Diagnosing Ion Interference in Isobaric Labeling Experiments
Isobaric labeling is a powerful strategy for quantitative mass spectrometry-based proteomic investigations. A complication of such analyses has been the co-isolation of multiple analytes of similar mass-to-charge resulting in the distortion of relative protein abundance measurements across samples. ...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2019-08-30 | MSV000084263 | MassIVE
UnknownQuantum Interference and Selectivity through Biological Ion Channels.
Not available
S-EPMC5278555 | biostudies-literature
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