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TranscriptomicsLaser capture microdissection of endothelial and neuronal cells from human dorsolateral prefrontal cortex
We used laser capture microdissection to isolate both microvascular endothelial cells and neurons from post mortem brain tissue from patients with schizophrenia and bipolar disorder and healthy controls. RNA was isolated from these cell populations, amplified, and analysed using Affymetrix HG133plus...
ORGANISM(S): Homo sapiens 
2008-12-18 | E-GEOD-12679 | biostudies-arrayexpress
TranscriptomicsMelampsora larici-populina gene expression in different tissues of uredinia
Laser Capture Microdissection (LCM) was used for expression analysis of three contrasted fungal tissues of uredinia corresponding respectively to spores and sporogenous hyphae, fungal structures in the spongy mesophyll and fungal infection structures in the palisade mesophyll. The array probes were ...
ORGANISM(S): Melampsora laricis-populina 
2010-07-22 | E-GEOD-21487 | biostudies-arrayexpress
TranscriptomicsTranscriptome of Ameloblastoma using Laser capture microdissection
The goal of the study was to characterize the whole genome transcriptome profiles of pure sample population of human ameloblastoma epithelial cells for examination of molecular pathways. Seventeen human ameloblastoma samples were obtained. Both fresh frozen and FFPE samples were used and placed in s...
ORGANISM(S): Homo sapiens 
2016-09-01 | E-GEOD-68531 | biostudies-arrayexpress
TranscriptomicsLaser Capture Microdissection and RNA Sequencing Human Adamantinomatous Craniopharyngioma
Laser capture microdissection of cluster cells, palisading epithelium and glial tissue from 2 cases of human adamantinomatous craniopharyngioma. 1 case performed in duplicate.
ORGANISM(S): Homo sapiens 
2018-04-11 | E-MTAB-5266 | biostudies-arrayexpress
TranscriptomicsLaser Capture Microdissection isolation of preovulatory granulosa cells from WT and bERKO ovaries
Determining the spatial and temporal expression of genes involved in the ovulatory pathway is critical for the understanding of the role of each estrogen receptor in the modulation of folliculogenesis and ovulation. Estrogen receptor (ER) b is highly expressed in ovarian granulosa cells and mice lac...
ORGANISM(S): Mus musculus 
2013-07-03 | E-GEOD-44651 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of effect of successive steps of laser capture microdissection using Rattus norvegicus liver samples treated with clofibrate
This is an evaluation of the impact of the successive steps of LCM procedure on gene expression profiling by comparing profiles from LCM samples to those obtained with non-microdissected liver samples collected after a one month CLO treatment in the rat. We showed that the H&E staining used for iden...
ORGANISM(S): Rattus norvegicus 
2007-10-11 | E-GEOD-691 | biostudies-arrayexpress
ProteomicsLow-melting point agarose as embedding medium for MALDI mass spectrometry imaging and laser-capture microdissection-based proteomics
We report an investigation of low-temperature embedding media in terms of their mechanical properties and embedding temperature, with the goal of determining a reliable embedding medium for the combination of MALDI MSI of lipids, laser-capture microdissection of tissue regions, and quantitative prot...
ORGANISM(S): Mus musculus (Mouse) 
2024-01-26 | PXD045349 | Pride
Proteomics Laser-capture microdissection Maldi msi Embedding media 
TranscriptomicsLaser Capture Microdissection of Hyperlipidemic Mouse Aorta Atherosclerosis
Atherosclerosis is a transmural chronic inflammatory condition of small and large arteries that is associated with adaptive immune responses at all disease stages. However, impacts of adaptive immune reactions on clinically apparent atherosclerosis such as intima lesion (plaque) rupture, thrombosis,...
ORGANISM(S): Mus musculus 
2011-11-03 | E-GEOD-21419 | biostudies-arrayexpress
TranscriptomicsCombining laser microdissection and RNA-seq to chart the transcriptional landscape of fungal development
RNA-seq analysis of developmental stages from the filamenous ascomycete Sordaria macrospora. Using laser capture microdissection, we separated protoperithecia (young fruiting bodies) from the surrounding hyphae. RNA isolation and amplification from 150 protoperithecia yields enough material for RNA-...
ORGANISM(S): Sordaria macrospora 
2012-10-01 | E-GEOD-33668 | biostudies-arrayexpress
TranscriptomicsGene Expression in Perivenous Hepatocytes: A Laser Capture Microdissection and Transcriptomic Analysis.
Differential expression of genes and proteins, as well as physiological processes, along the hepatic acinus, known collectively as hepatic zonation, is a fundamental feature of hepatic physiology. However, the precise cellular location of only a relatively small proportion of the ~14,000 genes expre...
ORGANISM(S): Mus musculus 
2013-06-26 | E-GEOD-48273 | biostudies-arrayexpress
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