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ProteomicsDevelopment of Photocrosslinking Probes Based on Huwentoxin-IV to Map the Site of Interaction on Nav1.7
This study reports on the design of a library of photoprobes based on the potent spider toxin Huwentoxin-IV and the determination of the toxin binding interface on VSD2 of Nav1.7 through a photocrosslinking and tandem mass spectrometry approach. Our Huwentoxin-IV probes selectively crosslink to extr...
ORGANISM(S): Homo sapiens (Human) 
2019-11-18 | PXD015037 | Pride
Nav1.7 Photoaffinity labelling Huwentoxin-iv Diazirine Peptide mapping 
UnknownTranspupillary collagen photocrosslinking for targeted modulation of ocular biomechanics.
Not available
S-EPMC8044034 | biostudies-literature
UnknownIdentification of photocrosslinking peptide ligands by mRNA display.
Not available
S-EPMC10232439 | biostudies-literature
ProteomicsA Photocrosslinking Probe to Capture the Substrates of Caseinolytic Protease P
Protein homeostasis in bacteria is regulated by proteases such as the tetradecameric caseinolytic protease P (ClpP). Although substrates of ClpP have been successfully deciphered in genetically engineered cells, methods which directly trap processed proteins within native cells remain elusive. Here,...
ORGANISM(S): Staphylococcus aureus 
2024-10-04 | PXD047274 | Pride
S. aureus Substrates 
UnknownPhotocrosslinking Activity-Based Probes for Ubiquitin RING E3 Ligases.
Not available
S-EPMC6963778 | biostudies-literature
ProteomicsIdentifying peptides and sites involved in FtsZ self-assembly in Escherichia coli through in vivo protein photocrosslinking of FtsZ residues mediated by the unnatural amino acid Bpa (p-benzoyl-L-phenylalanine), followed by tandem mass spectrometry analysis of purified FtsZ crosslinking dimers.
Bacterial cell division hinges on the Z-ring, an architecture built from the dynamical assembly and disassembly of FtsZ proteins. This delicate balance ensures not only apparent stability, but also continuous remodeling, both of which are required for Z-ring functioning. However, the molecular natur...
ORGANISM(S): Escherichia coli 
2025-05-10 | PXD063736 | Pride
Escherichia coli Ftsz Bacterial cell division P-benzoyl-l-phenylalanine Photocrosslinking 
UnknownPhotocrosslinking Probes Proximity of Thymine Modifiers Tethering Excitonically Coupled Dye Aggregates to DNA Holliday Junction.
Not available
S-EPMC9268628 | biostudies-literature
UnknownPatterned photocrosslinking to establish stiffness anisotropies in fibrous 3D hydrogels.
Not available
S-EPMC9552552 | biostudies-literature
UnknownMapping the binding interface of ERK and transcriptional repressor Capicua using photocrosslinking.
Not available
S-EPMC4507193 | biostudies-literature
UnknownEpitope-directed antibody selection by site-specific photocrosslinking.
Not available
S-EPMC7112767 | biostudies-literature
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