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ProteomicsPre-fractionation extends, but creates a bias in the detectable HLA class Ι ligandome
HLA class Ι molecules on the cell surface enable CD8+ T lymphocytes to recognize cellular alterations in the form of antigens, including mutations, protein copy number alterations, aberrant post-translational modifications or pathogen proteins. At any given moment, tens of thousands of different sel...
ORGANISM(S): Homo sapiens (Human) 
2019-02-25 | PXD011257 | Pride
Hla class ι ligandome  hla class ι peptide ligands  high ph reversed phase  strong cation exchange  pre-fractionation 
UnknownPre-fractionation Extends but also Creates a Bias in the Detectable HLA Class Ι Ligandome.
Not available
S-EPMC6456874 | biostudies-literature
UnknownA next generation setup for pre-fractionation of non-denatured proteins reveals diverse albumin proteoforms each carrying several post-translational modifications.
Not available
S-EPMC6692309 | biostudies-literature
ProteomicsSubCellBarCode: Integrated workflow for robust classification and visualization of spatial proteome
We have developed a mass spectrometry (MS) and bioinformatics-based pipeline to generate a proteome-wide resource of protein subcellular localization across multiple human cancer cell lines (www.subcellbarcode.org). Here, we present a detailed wet-lab protocol spanning from subcellular fractionation...
ORGANISM(S): Homo Sapiens (human) 
2022-09-26 | PXD022533 | JPOST Repository
ProteomicsProteomic profiling of over 13,000 proteins across ten mouse tissues using multi-dimensional peptide pre-fractionation
Proteome-wide abundance profiling across tissues can help to provide insight into the biological mechanisms underlying tissue-specific function and potential related dysfunction and amelioration thereof. Here, we use sample multiplexing to profile the proteomes of ten diverse mouse tissues using TMT...
ORGANISM(S): Mus musculus (Mouse) 
2025-11-18 | PXD061609 | Pride
Tmtpro Tissue-specific expression Strong anion exchange fractionation Real-time search Peptide sax 
Proteomicsdifferentially expressed N-glycopeptides in HepG2 related to LO2 cell line
With isotopically dimethyl labeling, ZIC-HILIC enrichment, C18 pre-fractionation, differentially expressed N-glycosylation in HCC HepG2 cells (relative to normal liver LO2 cells) were analyzed using penta-HILIC-MS/MS.
ORGANISM(S): Homo sapiens (Human) 
2019-05-31 | PXD012424 | Pride
N-glycopeptides Q excactive Human liver Hcc 
ProteomicsHuman Cardiac Stem Cells Receptome
Human cardiac stem cells (hCSC) express a portfolio of plasma membrane receptors that are involved in the regulatory auto/paracrine feedback loop mechanism of activation of these cells, and consequently contribute to myocardial regeneration. In order to attain a comprehensive description of hCSC rec...
ORGANISM(S): Homo sapiens (Human) 
2015-01-30 | PXD001117 | Pride
Proteomics Nanolc-ms/ms Receptome Human cardiac stem cells Pre-fractionation 
GenomicsGenome wide analysis of Saccharomyces nascent RNA
To address co-transcriptional pre-mRNA processing events, Saccharomyces nascent RNA was isolated by chromatin fractionation and analyzed on a genome-wide high density tiling microarray. Co-transcriptional splicing efficiencies were derived by determination of intronic relative to exonic sequence con...
ORGANISM(S): Saccharomyces cerevisiae Saccharomyces 
2010-11-26 | GSE24040 | GEO
TranscriptomicsStepwise development of hematopoietic stem cells from embryonic stem cells
The cellular ontogeny of hematopoietic stem cells (HSCs) remains poorly understood because their isolation from and their identification in early developing small embryos are difficult. We attempted to dissect early developmental stages of HSCs using an in vitro mouse embryonic stem cell (ESC) diffe...
ORGANISM(S): Mus musculus 
2009-03-18 | E-GEOD-15053 | biostudies-arrayexpress
TranscriptomicsArabidopsis mRNA export factor tho5ab: molecular interactions and role in heat stress responses
A central function in linking RNA polymerase II transcription and pre-mRNA processing with mRNA export is executed by the transcription and export (TREX) complex. TREX consists of a hexameric core termed THO that associates with various combinations of additional proteins giving rise to alternative ...
ORGANISM(S): Arabidopsis thaliana 
2026-02-03 | GSE308332 | GEO
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