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ProteomicsPuf3 Tap Tag and Mass Spectrometry in Not4 Delta and WT Yeast Strains
Investigation of the proteins associated with Puf3, an RNA-binding protein, in Saccharomyces cerevisiae. The goal is to compare the Puf3 interactome in a wild-type strain and in a Not4-deleted strain (Δnot4), in order to assess the potential role of Not4 in modulating Puf3-associated complexes. Puf3...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2026-09-14 | PXD069853 | Pride
Yeast puf3 tap tag not4 deletion wt ap mass spectrometry yeast 
ProteomicsInterrogating the interaction of fission yeast RNA-binding protein Puf3 with the Ccr4-Not complex using crosslinking mass spectrometry
mRNA decay is a key determinant of gene regulation, initiated by the shortening of mRNA poly(A) tails by the CCR4-NOT deadenylation complex. Specificity is achieved through RNA adaptors—RNA-binding proteins that recruit CCR4-NOT to specific substrate mRNAs in a regulated manner. However, the molecul...
ORGANISM(S): Schizosaccharomyces pombe 927 
2025-09-03 | PXD055147 | Pride
Crosslinking ms Idr Protein-protein interaction Rna binding protein puf3 Ccr4-not complex 
GenomicsPUF3 RIP and rRNA depletion control
PUF3 RIP and rRNA depletion control
PRJEB33093 | ENA
ProteomicsSchizosaccharomyces pombe Puf3 interactome
Puf3 is a RNA-binding protein, a member of the conserved Puf-protein family. Combining different functional genomics data, we have analyzed the role of Puf3 in post-transcriptional gene regulation in S. pombe. We present data on Puf3 interacting proteins and regulatory mRNA targets.
ORGANISM(S): Schizosaccharomyces pombe 972h- 
2022-03-23 | PXD006318 | Pride
Rip-chip Puf3 Tap purification Protein interactors Rna-binding protein Post-transcriptional regulation 
GenomicsPUF3 RNAi in bloodstream-form of Trypanosoma brucei
PUF3 RNAi in bloodstream-form of Trypanosoma brucei
PRJEB33062 | ENA
GenomicsPotential modulation of Puf3 chromatin association mediated by Rpb4
Potential modulation of Puf3 chromatin association mediated by Rpb4
PRJNA638298 | ENA
UnknownPuf3 participates in ribosomal biogenesis in malaria parasites.
Not available
S-EPMC5897713 | biostudies-literature
GenomicsRpb4 and Puf3
Rpb4 and Puf3
PRJNA638290 | ENA
UnknownRoles of the Pumilio domain protein PUF3 in Trypanosoma brucei growth and differentiation.
Not available
S-EPMC10317725 | biostudies-literature
GenomicsPuf3 participates in ribosomal biogenesis in malaria parasites
Puf3 participates in ribosomal biogenesis in malaria parasites
PRJNA414782 | ENA
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