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TranscriptomicsK562 stimulated with TNFα with translation blocking using Puromycin. 12 samples were taken with ChronoSeq and barcodes with unique time-tags
We applied ChronoSeq to profile inflammatory responses in K562 cells stimulated with TNF-α, to capture the rapid NF-κB signaling cascade. 10 minute interval between samples including a 1 minute sampling duration. In addition Puromycin was added at a does of 5μg/ml for blocking translation. The purpo...
ORGANISM(S): Homo sapiens 
2025-11-01 | E-MTAB-15947 | biostudies-arrayexpress
ProteomicsQuantitative nascent proteome profiling by pulse labeling of O-propargyl-puromycin and stable isotope labeled amino acids
Recently, a clickable puromycin analog, O-propargyl-puromycin (OPP), was developed and applied to label C-termini of nascent polypeptide chains (NPCs), followed by a proteomic analysis of NPCs that can be affinity purified via click reaction. Despite its advantage, the affinity purification of NPCs ...
ORGANISM(S): Homo Sapiens (human) 
2020-09-17 | PXD019459 | JPOST Repository
ProteomicsPuromycin acetyltransferase-mediated cell-specific labeling of de novo synthesized proteins, part I
Puromycin and its derivative O-propargyl puromycin (OPP) enable detection of nascent proteins. Use of these metabolic labels in complex mixtures of cells leads to indiscriminate tagging of nascent proteomes independent of cell type. Here we have used puromycin N-acetyltransferase (PAT), for cell-spe...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-10-24 | PXD043494 | Pride
Neuro2a Hek Opp-id Shotgun proteomics Streptavidin pulldown 
ProteomicsPuromycin acetyltransferase-mediated cell-specific labeling of de novo synthesized proteins, Part II
Puromycin and its derivative O-propargyl puromycin (OPP) enable detection of nascent proteins. Use of these metabolic labels in complex mixtures of cells leads to indiscriminate tagging of nascent proteomes independent of cell type. Here we have used puromycin N-acetyltransferase (PAT), for cell-spe...
ORGANISM(S): Rattus rattus (Black rat) 
2023-10-24 | PXD043498 | Pride
Opp-id Shotgun proteomics Streptavidin pulldown Rat cortical neuron-glia co-culture 
ProteomicsEffect of puromycin on newly synthesized proteome in pancreatic cancer cells
Nascent proteomic profiling of a translational program in response to puromycin treatment in pancreatic cancer cells
ORGANISM(S): Homo sapiens (Human) 
2026-08-15 | PXD062045 | Pride
Silac  newly synthesized protein  puromycin 
TranscriptomicsTranscription profiling of kidney in rats exposed to different doses of Puromycin - ILSI-HESI NephroToxicity Study: Compound Puromycin
Evaluation and comparison of biological responses of rats and gene expression responses of the kidney in rats exposed to different doses of Puromycin [CAS:53-79-2;CHEBI:17939]
ORGANISM(S): Rattus norvegicus 
2004-09-01 | E-TOXM-12 | biostudies-arrayexpress
TranscriptomicsFungenES: FunGenES_UKOE-4: Functional characterization and transcriptome analysis of embryonic stem cells-derived contractile smooth muscle cells
This experiment is part of the FunGenES project (FunGenES - Functional Genomics in Embryonic Stem Cells partially funded by the 6th Framework Programme of the EuropeanUnion, http://www.fungenes.org). The experiment was conducted at University of Cologne, Cologne, Germany. Goal of the experiment is a...
ORGANISM(S): Mus musculus 
2011-02-25 | E-TABM-1123 | biostudies-arrayexpress
TranscriptomicsEverolimus protects podocytes via stabilizing TUBB2B and DCDC2 expression
Glomerular podocytes are highly differentiated cells that are key components of the kidney filtration units. The podocyte cytoskeleton builds the basis for the dynamic podocyte cytoarchitecture and plays a central role for proper podocyte function. Recent studies implicate that immunosuppressive age...
ORGANISM(S): Homo sapiens 
2015-09-09 | E-GEOD-66107 | biostudies-arrayexpress
TranscriptomicsLooking for mitochondrial associated RNA
We investigated the peripheral mitochondrial localization of nuclear-encoded mRNAs (MLR) in various conditions in which translation was inhibited or the mRNA binding protein context altered (Delta puf3). We used cell fractionation protocols together with microarray to assess the distribution of mRNA...
ORGANISM(S): Saccharomyces cerevisiae 
2008-06-01 | E-GEOD-9393 | biostudies-arrayexpress
UnknownPuromycin-sensitive aminopeptidase: an antiviral prodrug activating enzyme.
Not available
S-EPMC2830364 | biostudies-literature
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