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TranscriptomicsLong non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR
Long non-coding RNAs (lncRNAs) form a new class of RNA molecules implicated in various aspects of protein coding gene expression regulation. To study lncRNAs in cancer, we generated expression profiles for 1708 human lncRNAs in the NCI60 cancer cell line panel using a high-throughput nanowell RT-qPC...
ORGANISM(S): Homo sapiens 
2016-06-29 | E-GEOD-80332 | biostudies-arrayexpress
TranscriptomicsHuman p53 signaling qPCR primer Array
H1299 cells were overexpressed miR-138 or silenced AGO2. The expression of 92 genes associated with p53 using the “Human p53 Signaling Pathway PCR Array” qPCR gene expression profiling. H1299 cells were transfected with NC mimics, AGO2 siRNA or miR-138 for 48h. Equal amount total RNA from each group...
ORGANISM(S): Homo sapiens 
2015-06-05 | E-GEOD-69562 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse line with high fertiliy by microarray analysis and qPCR.
The objective of the present study was to identify genes that are involved in increasing the ovulation number in mouse line FL1 that had been selected for high fertility performance. Experiment Overall Design: Ovaries from 30 animals of line FL1 and of the control line DUKsi were collected at the me...
ORGANISM(S): Mus musculus 
2008-10-25 | E-GEOD-11113 | biostudies-arrayexpress
TranscriptomicsBilberries reduce low-grade inflammation and subacute endotoxemia in individuals with features of metabolic syndrome - microarray/QPCR profiling of dietary effects of bilberries in PBMCs
Obesity and its consequences on cardiometabolic health have been associated to low-grade inflammation. The most diverse source of dietary anti-inflammatory compounds is polyphenols and especially anthocyanins, which are major polyphenols in bilberries (Vaccinium myrtillus). We investigated the effec...
ORGANISM(S): Homo sapiens 
2012-09-15 | E-GEOD-34145 | biostudies-arrayexpress
GenomicsqPCR profiling of mouse immune cell miRNAs
qPCR profiling of mouse immune cell miRNAs
PRJNA602706 | ENA
TranscriptomicsExpression data from peripheral blood mononuclear cells of subjects supplemented with vitamin C [qPCR array analysis]
A role of vitamin C(ascorbic acid) as an antioxidant molecule has been recognized, largely based on in vitro studies. However, more recently, the concept of M-bM-^@M-^\antioxidant moleculeM-bM-^@M-^] has been reconsidered and its biological function is no longer considered to be simply due to its ab...
ORGANISM(S): Homo sapiens 
2014-03-01 | E-GEOD-54476 | biostudies-arrayexpress
GenomicsQuantification of fungal dark matter using qPCR
Quantification of fungal dark matter using qPCR
PRJEB69215 | ENA
TranscriptomicsHeterogeneity in early lymphoid compartments [qPCR]
In order to understand the developmental trajectories of early lymphocyte development it is crucial to prospectively isolate stage and lineage specific cells. It has become clear that early lymphoid progenitor compartments in the bone marrow are molecularly and functionally heterogeneous which warra...
ORGANISM(S): Mus musculus 
2016-10-23 | E-GEOD-74606 | biostudies-arrayexpress
ProteomicsUsing RT-qPCR, proteomics and microscopy to unravel the spatio-temporal expression and subcellular localization of hordoindolines across development in barley endosperm
Hordeum vulgare (barley) hordoindolines (HINs), HINa, HINb1 and HINb2, are orthologous proteins of wheat puroindolines (PINs) that are small, basic, cysteine-rich seed-specific proteins and responsible for grain hardness. Grain hardness, is, next to its protein content, a major quality trait. In ba...
ORGANISM(S): Hordeum vulgare (Barley) 
2018-07-02 | PXD009708 | Pride
Spatio-temporal  laser microdissection  endosperm  label-free shotgun proteomics  rt-qpcr  reference genes  end-product quality Golden promise  protein bodies  grain hardness Barley caryopsis  hordoindoline 
TranscriptomicsHuman Wnt signaling pathway (Realtime qPCR array)
Human bone marrow mesenchymal stem cells (BMMSC) and human embryonic mesenchymal stem cells (ESMSC)were used. X-ray irradiation (2Gy) or 0Gy was delivered. After 4 hours, equal amount total RNA from each sample was extracted prior to gene expression analysis. After making cDNA, genes associated with...
ORGANISM(S): Homo sapiens 
2014-08-12 | E-GEOD-60310 | biostudies-arrayexpress
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