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Total proteome for triplicates of WT and RBPMS KO hESCs to identify differences in proteins abundances due to a loss of RBPMS. Cells were lysed and prepared according to the in solution digest protocol and 50 µg proteins were reduced, alkylated and digested with Lys-C and trypsind.
ORGANISM(S): Homo sapiens (Human) 
2023-05-10 | PXD032045 | Pride
RBPMS regulates alternative splicing decision via recruiting other trans factors. This experiment aims to investigate the interactome of Strep-II tagged recombinant RBPMS in Hela nuclear extract. The interactome of the full length protein is compared to the construct lacking the far C-terminal 20 am...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD037620 | Pride
RBPMS in Heart Failure during aging
RBPMS regulate splicing decisions by modulating the transcript-bound proteome. This experiment aims to investigate the proteome assembled on an experimental RNA, TM3 transcript, in human Hela nuclear extract under the various conditions, ± ATP and ± RBPMS. The model RNA is tethered on amylose bead v...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD037617 | Pride
Transcriptome analysis of embryonic hearts from germline RBPMS knockout mutants
RBPMS regulates cardiomyocyte contraction and cardiac function through RNA alternative splicing
RBPMS knockdown and overexpression in rat PAC1 pulmonary artery smooth muscle cells (SMCs)
Immunoprecipitation for RBPMS in human embryonic stem cells. Triplicates of sheep anti-rabbit beads coupled with rabbit anti-RBPMS antibody were compared with and triplicates sheep anti-rabbit beads, which served as IgG control. Bands above heavy IgG, between heavy IgG and light IgG, below IgG and b...
ORGANISM(S): Homo sapiens (Human) 
2023-10-26 | PXD032040 | Pride
RBPMS and RBPMS2 share a high amino acid sequence similarity and are interchangeable in in vitro splicing assays. However, it remains unclear whether RBPMS and RBPMS2 play similar or distinct function during heart development in vivo. To address this question, we constructed Rbpms and Rbpms2 floxed ...
ORGANISM(S): Mus musculus 
2025-08-07 | GSE280824 | GEO
RBPMS and RBPMS2 share a high amino acid sequence similarity and are interchangeable in in vitro splicing assays. However, it remains unclear whether RBPMS and RBPMS2 play similar or distinct function during heart development in vivo. To address this question, we constructed Rbpms and Rbpms2 floxed ...
ORGANISM(S): Mus musculus 
2025-08-07 | GSE280806 | GEO
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