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MetabolomicsGC-XLE method development: dSPE and MgSO4 as clean-up for sample preparation
Not available
ORGANISM(S): Homo sapiens 
ST001787 | MetabolomicsWorkbench
ProteomicsArabidopsis thaliana peptide SP2 and ethyl acetate extraction clean up.
The success of bottom-up proteomic analysis frequently depends on the efficient removal of contaminants from protein or peptide samples before LC-MS/MS. For a peptide clean-up workflow, the single-pot solid-phase-enhanced peptide sample preparation on carboxylate-modified paramagnetic beads (termed ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-01-03 | PXD045031 | Pride
Arabidopsis thaliana Peptide clean-up Ethyl acetate extraction Sp2 Polyethylene glycol Sodium dodecyl sulfate Magnetic beads Lc-ms/ms 
UnknownAn Automated Centrifugal Microfluidic Platform for Efficient Multistep Blood Sample Preparation and Clean-Up towards Small Ion-Molecule Analysis.
Not available
S-EPMC10745919 | biostudies-literature
UnknownAn Automated and High-Throughput Immunoaffinity Magnetic Bead-Based Sample Clean-Up Platform for the Determination of Aflatoxins in Grains and Oils Using UPLC-FLD.
Not available
S-EPMC6832433 | biostudies-literature
UnknownIntegrated preservation and sample clean up procedures for studying water ingestion by recreational swimmers via urinary biomarker determination.
Not available
S-EPMC5937129 | biostudies-literature
Proteomics3D-Printed Device for Consistent and High-Throughput Peptide Sample Clean-up Using StageTips
Mass spectrometry (MS) is a fundamental technology for the study of proteomics. A core technique for MS analysis is solid-phase extraction (SPE) for the removal of contaminants and sample concentration, and StageTips are one of the most commonly used devices used for SPE in modern proteomics workflo...
ORGANISM(S): Homo sapiens (Human) 
2019-03-21 | PXD009324 | Pride
3d-printed Stagetip 96-well 
UnknownA microfluidic platform integrating paper adsorption-based sample clean-up and voltage-assisted liquid desorption electrospray ionization mass spectrometry for biological sample analysis.
Not available
S-EPMC7275932 | biostudies-literature
ProteomicsSolvent Precipitation SP3 (SP4) enhances recovery for proteomics sample preparation without magnetic beads
Complete, reproducible extraction of protein material is essential for comprehensive and unbiased proteome analyses. A current gold standard is single-pot, solid-phase-enhanced sample preparation (SP3), in which organic solvent and magnetic beads are used to denature and capture protein aggregates, ...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) Drosophila melanogaster (Fruit fly) 
2022-08-01 | PXD032095 | Pride
Microparticles Proteomics Sample preparation Protein aggregation Contaminant removal Lc−ms/ms Protein precipitation 
ProteomicsDifferences in Protein Capture by SP3 and SP4 Demonstrate Mechanistic Insights of Proteomics Clean-up Techniques.
The goal of proteomics experiments is to identify proteins to observe changes in cellular processes and diseases. One challenge in proteomics is the removal of contaminants following protein extraction, which can limit protein identifications. Single-pot, solid-phase-enhanced sample preparation (SP3...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-02-20 | MSV000094130 | MassIVE
UnknownImmunocapture sample clean-up in determination of low abundant protein biomarkers - a feasibility study of peptide capture by anti-protein antibodies.
Not available
S-EPMC9097496 | biostudies-literature
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