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TranscriptomicsPromoter directionality is controlled by U1 snRNP and polyadenylation signals
Transcription of the mammalian genome is pervasive, but productive transcription outside of protein-coding genes is limited by unknown mechanisms. In particular, although RNA polymerase II (RNAPII) initiates divergently from most active gene promoters, productive elongation occurs primarily in the s...
ORGANISM(S): Mus musculus 
2013-06-28 | E-GEOD-46433 | biostudies-arrayexpress
ProteomicsSequence specific recognition of U1 snRNP stem-loop 4 by the ubiquitin-like domain of U2 snRNP protein SF3A1 and its RGG motif
Cross-linking of isotope-labelled RNA coupled with mass spectrometry (CLIR-MS) was used to study the interaction between the ubiquitin-like domain (UBL) of the protein SF3A1, a component of the U2 snRNP, with stem-loop 4 RNA (SL4) from the U1 snRNP. The complex was cross-linked and analysed both in ...
ORGANISM(S): Homo sapiens (Human) 
2022-02-22 | PXD027189 | Pride
Uv cross-linking  sf3a1  u1  u2  snrnp  splicing  spliceosome  structural proteomics  protein-rna  rna-protein  protein-rna complex  rna-protein complex  clir-ms 
TranscriptomicsiCLIP and NGS to map protein-RNA interactions for U1-snRNP proteins in Trypanosoma brucei
Individual-nucleotide resolution UV-crosslinking and immunoprecipitation (iCLIP) combined with high-throughput sequencing was performed to generate genome-wide binding maps of two U1-snRNP proteins: U1C and U1-70K in Trypanosoma brucei. 3 (2) biological replicates of U1C (U1-70K) -specific co-immuno...
ORGANISM(S): Trypanosoma brucei 
2014-05-16 | E-GEOD-43848 | biostudies-arrayexpress
ProteomicsComposition and processing activity of a semi-recombinant holo U7 snRNP
In animal cells, replication-dependent histone pre-mRNAs are cleaved at the 3’ end by U7 snRNP consisting of two core components: a ~60-nucleotide U7 snRNA and a ring of seven proteins, with Lsm10 and Lsm11 replacing the spliceosomal SmD1 and SmD2. Lsm11 interacts with FLASH and together they recrui...
ORGANISM(S): Mus musculus (Mouse) 
2019-11-25 | PXD014164 | Pride
Sm proteins U7 snrnp Cpsf73 Lsm11 Flash Histone pre-mrna 3’ end processing 
TranscriptomicsNuclear Matrix Factor hnRNP U/SAF-A Exerts a Global Control of Alternative Splicing by Regulating U2 snRNP Maturation
The nuclear matrix associated hnRNP U/SAF-A protein has been implicated in diverse pathways from transcriptional regulation to telomere length control to X inactivation, but the precise mechanism underlying each of these processes has remained elusive. Here, we report hnRNP U as a regulator of SM...
ORGANISM(S): Homo sapiens 
2012-02-16 | E-GEOD-34491 | biostudies-arrayexpress
ProteomicsU1 snRNP and RNA polymerase II interaction is predominantly mediated by Prp40 rather than U1-70K in yeast
Transcription and splicing are coupled both temporally and physically. A previous cryo-EM structure of the human U1 snRNP and RNA polymerase pol II complex has shown that U1 snRNP uses predominantly the RRM domain of U1-70K to directly interact with the RPB2 subunit of pol II. However, residues on U...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2026-05-20 | PXD078573 | Pride
Yeast Spliceosome Splicing Pol ii Snrnp Prp40 
GenomicsNeuronal stimulation changes U1 snRNP binding and intronic polyadenylation
Neuronal stimulation changes U1 snRNP binding and intronic polyadenylation
PRJNA770346 | ENA
TranscriptomicsIdentification of RNAs associated with the Caenorhabditis elegans SL1 snRNP SNA-1 protein by RIP-Seq
This study aimed to identify RNAs bound to the C. elegans SL1 snRNP SNA-1 protein through use of RNA immunoprecipitation sequencing. Three IP sample replicates and three control sample replicates were sequenced.
ORGANISM(S): Caenorhabditis elegans 
2022-06-07 | E-MTAB-10289 | biostudies-arrayexpress
GenomicsThe Arabidopsis U1 snRNP regulates mRNA 3’-end processing
The Arabidopsis U1 snRNP regulates mRNA 3’-end processing
PRJEB65251 | ENA
GenomicsU1 snRNP regulates alternative promoter activity by inhibiting premature polyadenylation
U1 snRNP regulates alternative promoter activity by inhibiting premature polyadenylation
PRJNA1200036 | ENA
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