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MetabolomicsIsotopomer analysis of MCF-7 breast cancer cell line incubated with 13C labelled glucose (GC-MS assay)
MS and NMR are two of the most powerful spectroscopic techniques used in the field of fluxomics, to analyze the flux distributions in cellular metabolomic pathways. Both of them possess pros and cons, such as, MS being the most sensitive and low-cost technique, while needs extensive sample processin...
2018-05-02 | MTBLS182 | MetaboLights
Metabolomics13C-glucose tracing analysis of IFN-treated A549 cells expressing wild-type SARS-CoV-2 Nsp3-4 or a deISGylation-defective Nsp3-4 mutant protein

Interferon (IFN)-stimulated gene 15 (ISG15) regulates diverse cellular processes, including antiviral immunity, through its conjugation to target proteins (ISGylation). Increasing evidence suggests that ISGylation can also reshape cellular metabolism; however, how viruses counteract ISGylation-me...

2026-05-05 | MTBLS14030 | MetaboLights
MetabolomicsThe effects of oligomycin administration and glutamine deprivation on metabolic profile of MCF7 breast cancer cell line measured using [1,2-13C2]-D-glucose and [U-13C5]-L-glutamine as tracers
This study aims to establish a comprehensive, genome-scale metabolic model (GSMM) combining different omics data of different regulatory levels of breast cancer cells in order to elucidate the way that these regulatory levels affect or determine each other. Together with transcriptomics, proteomics ...
2021-01-18 | MTBLS183 | MetaboLights
MetabolomicsStable isotope resolved analysis of HUVEC Cells under normaxia and hypoxia
In order to analyze HUVECs under normoxia and hypoxia, cells were initially deprived of excess nutrients and hormones by maintaining them overnight in a restricted medium with only 2% fetal bovine serum, and incubated for 40 hours in the presence of the tracer [1,2-13C2]-glucose until sample collect...
2017-12-18 | MTBLS412 | MetaboLights
ProteomicsData-independent proteomic profiling of Escherichia coli across graded 13C abundance treatments using Astral LC-MS/MS
This dataset contains data-independent acquisition (DIA) LC-MS/MS measurements of Escherichia coli samples acquired across a gradient of 13C abundance treatments. The treatment groups correspond to 1%, 2%, 5%, 25%, 50%, and 99% 13C abundance, with three replicate runs collected for each treatment le...
ORGANISM(S): Escherichia coli Bacteria 
2026-09-08 | PXD075964 | Pride
13c abundance Proteomics Dia Stable isotope probing Sip Escherichia coli Astral Evosep one Label-free Orbitrap Lc-ms/ms 
MetabolomicsHierarchical Carbon Metabolism in Iron-Deficient Bacteria Favors Iron-Scavenging Strategy

High-affinity iron (Fe) scavenging compounds, or siderophores, are widely employed by soil bacteria to survive scarcity in bioavailable Fe. Siderophore biosynthesis relies on cellular carbon metabolism, despite reported decrease in both carbon uptake and Fe-containing metabolic proteins in Fe-def...

2020-12-05 | MTBLS1715 | MetaboLights
MetabolomicsRegulatory mechanisms for aromatic carbon metabolism in Comamonas testosteroni
Widely reported discrepancies between metabolic flux and transcripts or enzyme levels in bacterial metabolism imply complex regulation mechanisms need to be considered, especially in new bacterial platforms for bioremediation and bioproduction. Comamonas testosteroni strains, which metabolize variou...
2022-11-21 | MTBLS3947 | MetaboLights
ProteomicsQuantitative metaproteomic analysis of 13C E. coli spike-in mixtures across dynamic range using Orbitrap Astral DIA LC-MS/MS
This dataset contains label-free DIA LC-MS/MS measurements of controlled bacterial spike-in samples designed to evaluate quantitative performance across a wide dynamic range. The samples consist of defined mixtures of 13C-labeled E. coli peptides spiked into unlabeled backgrounds at different ratios...
ORGANISM(S): gut metagenome 
2026-08-31 | PXD078868 | Pride
13c labeling Dynamic range Dia Stable isotope probing Label-free proteomics Benchmarking Orbitrap astral E. coli spike-in Metaproteomics 
MetabolomicsA Comprehensive and Ultrasensitive Isotope Calibration Method for Soil Amino Compounds Using Orbitrap Mass Spectrometry
Bound amino compounds (amino acid and amino sugar polymers) comprise a significant proportion (~40%) of soil organic nitrogen and therefore represent an essential source of nitrogen for plant and microbial nutrition. The analysis of their content and isotope enrichment still represents a significant...
2025-06-16 | MTBLS12550 | MetaboLights
UnknownHyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging.
Not available
S-EPMC5226623 | biostudies-literature
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