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ProteomicsQuantitative proteomic comparison between wild type Col and acinus-2 pinin-1 mutant using 15N metabolic labeling
ACINUS and PININ genes regulate transcription and alternative splicing (Bi et al., 2021) and we hypothesize that some proteins should have altered expression in the double mutant. Therefore, MS experiments were designed to identify these altered proteins on a global scale using 15N metabolic labeli...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-02-04 | PXD030081 | Pride
Arabidopsis Acinus Quantification 15n metabolic labeling Protein prospector Pinin 
ProteomicsQuantitative proteomic comparison between wild type Col and sec-5 mutant using 15N metabolic labeling
SECRET AGENT (SEC) is an O-GlcNAc transferase involved in O-GlcNAcylation of many proteins. However, sec-5 null allele mutant phenotype is very subtle. To examine if there are proteins that show altered expression in the sec-5 mutant compared to wild-type Col, we designed MS experiments to identify ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-02-04 | PXD030575 | Pride
Arabidopsis Quantification 15n metabolic labeling Protein prospector Secret agent (sec) 
ProteomicsCombined 15N-Labelling and TandemMOAC Quantifies Phosphorylation of MAP Kinase Substrates Downstream of MKK7 in Arabidopsis
Reversible protein phosphorylation is a widespread posttranslational modification that plays a key role in eukaryotic signal transduction. Due to the large dynamic range of protein abundance, low stoichiometry and transient nature of protein phosphorylation, the detection and accurate quantification...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-01-02 | PXD008200 | Pride
Phosphoproteomics Tandem moac 15n labeling Mapk Mpk substrate 
ProteomicsUsing targeted quantification (PRM) to quantify proteins between wild type Col and acinus-2 pinin-1 mutant with 15N metabolic labeling
ACINUS and PININ genes regulate transcription and alternative splicing (Bi et al., 2021) and we hypothesize that some proteins should have altered expression in the double mutant. Therefore, MS experiments were designed to identify these altered proteins on a global scale using 15N metabolic labeli...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-04-19 | PXD030096 | Pride
Arabidopsis Acinus Quantification 15n metabolic labeling Protein prospector Targeted quantification Pinin Parallel reaction monitoring (prm) 
Proteomics15N labeling of mouse microbiota
This study investigated the in vivo dynamics of the microbiome using metaproteomics coupled with protein-SIP in mice.
ORGANISM(S): gut metagenome 
2021-08-26 | PXD017451 | Pride
Microbiota Mouse 15n stable isotope labeling Metaproteomics 
ProteomicsParallel Reaction Monitoring of 42 peptide pairs with 15N metabolic labeling
To examine the properties of 14N and 15N labeled peptides from 15 metabolic labeling samples, we set up targeted quantification using Parallel Reaction Monitoring (PRM) on a high resolution, high accuracy mass spectrometer. We first examined the co-elution of light and heavy peptide forms. Furthermo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-06-09 | PXD030112 | Pride
Arabidopsis Quantification 15n metabolic labeling Protein prospector Targeted quantification Parallel reaction monitoring (prm) 
Unknown15N metabolic labeling of mammalian tissue with slow protein turnover.
Not available
S-EPMC2527585 | biostudies-literature
ProteomicsRelative quantification of 15N labeled acinus-2 pinin-1 and 14N labeled Col through Data Dependent Acquisition to obtain peptide information for targeted Parallel Reaction Monitoring experiment
To examine the properties of 14N and 15N labeled peptides using Parallel Reaction Monitoring (PRM) on a high resolution, high accuracy mass spectrometer we first set up this DDA experiment. The purpose of this experiment was to choose peptides that are good candidates for targeted quantification. Th...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-06-09 | PXD030098 | Pride
Arabidopsis Quantification Data dependent acquisition 15n metabolic labeling Relative quantification Silac Protein prospector 
ProteomicsA protocol for global quantification of phosphoproteins combining metabolic labeling and gel-based proteomics
Differential proteomics targeting the abundance level and changes of proteins is commonly used in biology to determine changes induced by different physiological states in living organisms. Here, current methods comprise both label-free and label-based proteomics approaches. More specifically, besid...
ORGANISM(S): Bacillus pumilus 
2017-10-02 | PXD005262 | Pride
Peroxide stress adaptation Gel-based proteomics Phosphorylation 14n/15n metabolic labeling Bacillus pumilus Quantification 
Proteomics15N Stable-isotope-labeling in Arabidopsis (SILIA) quantitative MS analysis of the AtACINUS interactome
AtACINUS protein is involved in regulation of alternative transcription and splicing(AS). Identifying interaction partners and protein complex compositions for AtACINUS can produce valuable information on the mechanisms by which they regulate transcription and AS, as well as post-translational modif...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-12-02 | PXD020700 | Pride
Seedling Acinus Plant Arabidopsis thaliana Atacinus Co-immunoprecipitation 15n stable-isotope-labeling in arabidopsis(silia) Ip-ms Lc-ms/ms 
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