Sort   by:  
 Page size 
MetabolomicsComparative non-targeted LC-MS metabolomic profiling of murine bone marrow-derived macrophages cultured in 2D versus 3D

Macrophages are key drivers of inflammatory and fibrotic diseases and their activation is shaped by interactions in their tissue microenvironment. However, dissecting the processes that drive immunopathology has proved challenging as traditional 2D culture methods fail to capture the complex mole...

2026-06-08 | MTBLS12917 | MetaboLights
ProteomicsLabel-free quantification of 3D MCF10A cell culture proteome (DIA)
Global shotgun proteomic analysis using data independent acquisition (DIA) and Spectronaut analysis on the input cell lysates before the enrichment step in proximity labeling experiments, specifically MCF10A cells stably expressing miniTurbo-BAD in 3D with biotin treatment (n=4)
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-05-21 | MSV000094825 | MassIVE
TranscriptomicsTranscription profiling by array of human stromal cells cultured with or without 3D BPH-1 prostate epithelial cells
BPH-1 prostate epithelial cells when cultured in Matrigel form 3D acini which closely recapitulate the tissue architecture of the prostate. The presence of primary stroma increases epithelial adhesions and causes changes in morphology. To identify the stromal signals which control actin/cadherin dy...
ORGANISM(S): Homo sapiens 
2010-06-09 | E-MEXP-2657 | biostudies-arrayexpress
ProteomicsMicroenvironment remodeling during human iPSC-derived NSC 3D differentiation
Brain microenvironment plays an important role in neurodevelopment and function, where extracellular matrix (ECM) components and soluble factors modulate cellular features, as migration, proliferation survival and neuronal function. Disruption of microenvironment’s homeostasis is often related to pa...
ORGANISM(S): Homo sapiens (Human) 
2018-07-31 | PXD007130 | Pride
3d cultures  neual differentiation  hipsc-derived nsc  ecm  microenvironment  swath 
GenomicsExpression profiles of lncRNAs in 3D cultures.
Expression profiles of lncRNAs in 3D cultures.
PRJNA852280 | ENA
ProteomicsAnalysis of extracellular matrix proteins from 3D cultures of primary prostate fibroblasts, DU145 cells and mixed cells
Composition and organization of extracellular matrix (ECM) in tumor stroma are important regulators of cancer cell behavior. Therefore, ECM produced by cancer associated fibroblasts is often used in in vitro experiments. Here, a proteome wide analysis of ECM proteins in 3D culture is analysed.
ORGANISM(S): Homo sapiens (Human) 
2018-03-13 | PXD006563 | Pride
3d culture Prostate cancer Fibroblast Extracellular matrix 
MetabolomicsLiquid chromatography coupled to high-resolution mass spectrometry metabolomics: A useful tool for investigating tumor secretome based on a three-dimensional co-culture model

Three-dimensional (3D) cell culture technologies, which more closely mimic the complex microenvironment of tissue, are being increasingly evaluated as a tool for the preclinical screening of clinically promising new molecules, and studying of tissue metabolism. Studies of metabolites released int...

2023-12-12 | MTBLS5375 | MetaboLights
ProteomicsConditions for maintenance of hepatocyte differentiation and function in 3D cultures
3D spheroid cultures of primary human hepatocytes (PHH) are used in studies of hepatic drug metabolism and toxicity. However, the 3D spheroids are maintained under different conditions, with possible cofounding results. Here we performed an in-depth analysis of how various culture conditions influen...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD024632 | Pride
Primary human hepatocyte culture Lc-ms/ms 
GenomicsHippo Signaling Regulates Spontaneous Human Trophoblast Syncytialization in 3D Cultures
Hippo Signaling Regulates Spontaneous Human Trophoblast Syncytialization in 3D Cultures
PRJNA1248008 | ENA
ProteomicsExtracellular vesicles secreted by 3D tumor organoids are enriched for immune regulatory signaling biomolecules compared to conventional 2D glioblastoma cell systems
Background: Newer 3D culturing approaches are a promising way to better mimic the in vivo tumor microenvironment and to study the interactions between the heterogeneous cell populations of glioblastoma multiforme. Like many other tumors, glioblastoma uses extracellular vesicles as an intercellular c...
ORGANISM(S): Homo sapiens (Human) 
2024-04-09 | PXD047328 | Pride
Ev Extracellular vesicle Proteomics Microrna 3d organoid model Brain cancer Glioblastoma multiforme 
Sort   by:  
 Page size