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MetabolomicsThe effect of the MYH9 in endothelial cells on metabolism

This document focuses on exploring the specific impact of MYH9 on the metabolic processes of vascular endothelial cells. To achieve this, a well-designed experimental approach was adopted: human umbilical vein endothelial cells (HUVECs) were subjected to different treatments using adenoviruses, i...

2026-03-05 | MTBLS12819 | MetaboLights
TranscriptomicsRemodeling of cell-type specific transcriptomes upon T cell stimulation in Systemic lupus erythematosus versus Sjögren’s syndrome
The RNA-sequencing to profile patient peripheral blood mononuclear cell (PBMC) samples of systemic lupus erythematosus (SLE, N=25), primary Sjögren’s syndrome (pSS, N=23), and healthy control (HD, N=24). We collected on the steady-state and 18-hours anti-CD3 culture to identify the transcriptome rem...
ORGANISM(S): Homo sapiens 
2023-12-20 | E-MTAB-12203 | biostudies-arrayexpress
ProteomicsDifferentially expressed proteins in Human Primary Keratinocytes upon IL-9 stimulation
HPKs from 3-5 healthy individual donors were pre-stimulated with TGFb (10ng/ml) plus IL-4 (20ng/ml) to upregulated the IL-9R (Das et al., 2019). Next day, HPKs were stimulated with IL-9 (50ng/ml) or in combination with IFNg (10ng/ml) for 24 hours at 37 degree C with 5% CO2. Sample preparation for L...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-01-28 | MSV000086776 | MassIVE
TranscriptomicsSingle-cell RNA sequencing of OT-I CD8+ T cells after stimulation with different affinity ligands
Activation of CD8+ T cells depends exquisitely on the affinity of the T cell receptor (TCR) for a peptide MHC (pMHC) ligand complex. Here, we activated OT-I transgenic CD8+ T cells with pure peptide and examined early activation responses by single-cell RNA-sequencing. T cells were activated with th...
ORGANISM(S): Mus musculus 
2018-06-25 | E-MTAB-6051 | biostudies-arrayexpress
TranscriptomicsChromatin immunoprecipitation of Foxp3 in mouse T-cell hybridoma cells
The forkhead transcription factor Foxp3 is essential for the development of CD4+CD25+ regulatory T (Treg) cells and prevention of autoimmunity, but how it controls the Treg gene expression program is not understood. We describe here genome-wide location and expression data that identify Foxp3 targe...
ORGANISM(S): Mus musculus 
2007-01-27 | E-TABM-154 | biostudies-arrayexpress
ProteomicsLabel-free proteomics to study the effect of IL-9 on MDA-MB-231 and HeLa cells
To study the proteomic profile of HeLa and MDA-MB-231 upon IL-9 stimulation, we treated both the cell-lines with the cytokine for 24 hours. Quantitative label-free proteomic analysis was then performed and abundance ratios were obtained for proteins from cells in IL-9 treated v. untreated conditions...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-03-03 | MSV000086990 | MassIVE
ProteomicsHiRIEF-LC-MS based proteomics analysis of subcellular fractions of human CD4+ T-Cells with and without T-cell Receptor stimulation.
Subcellular proteomics analysis of human T-cells in response to T-cell receptor stimulation. High Resolution Isoelectric Focusing (HiRIEF) LC-MS/MS and relative quantification by TMT 10-plex was used to analyze subcellular fractions (Cytosol, Nuclear and Mitochondria-Small Organelle-Membrane) of Hum...
ORGANISM(S): Homo sapiens (Human) 
2019-11-13 | PXD013284 | Pride
Subcellular T-cell Human T-cell receptor 
TranscriptomicsTranscription profiling of mouse cytotoxic T lymphocyte cell line CTLL-2 treated with interleukin-2
The cytokine IL-2 determines T cell fate by controlling T cell proliferation and differentiation, but the expression files of IL-2 regulated genes are not defined; Using Affymetrix mouse genome array and mouse T cell line CTLL-2, we analyzed the expression profiles of IL-2 regulated genes over 10 ti...
ORGANISM(S): Mus musculus 
2008-11-22 | E-GEOD-6085 | biostudies-arrayexpress
ProteomicsQuantitative interactomics of Lck-TurboID in living human T cells unveils T cell receptor stimulation-induced proximal Lck interactors
While Lck has been widely recognized to play a pivotal role in the initiation of the T cell receptor (TCR) signaling pathway, an understanding of the precise regulation of Lck in T cells upon TCR activation remains elusive. Investigation of protein-protein interaction (PPI) using proximity labeling ...
ORGANISM(S): Homo sapiens (Human) 
2020-11-12 | PXD020759 | Pride
Proximity labeling T cell receptor Lck Turboid Interactome 
TranscriptomicsDynamics of Virus-specific CD8+ T cells in the Human Nasal Cavity
The goal of this experiment is to compare the transcriptomic profile of CD8+ T cells isolated from the nasal cavity and whole blood at two different state, baseline and CD3/28 activated. This will allow us to characterise the unique features of CD8+ T cells in the nasal cavity. We isolated CD8+CD4...
ORGANISM(S): Homo sapiens 
2025-08-23 | E-MTAB-15436 | biostudies-arrayexpress
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