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ProteomicsLck phosphorylation status of Lck Tyr192 and Tyr192/Tyr505 mutantsLck phosphorylation status of Lck Tyr192 and Tyr192/Tyr505 mutants
Lymphocyte-specific protein tyrosine kinase (Lck) is crucial for signaling from the T cell receptor (TCR) and is controlled through tyrosine phosphorylations. Phosphorylated Tyr505 (pTyr505) promotes a closed, inactive conformation of Lck, while pTyr394 is critical for kinase activity. Additionally,...
ORGANISM(S): Homo sapiens (Human) 
2021-04-02 | PXD014470 | Pride
T-cells Tyrosine phosphorylation Lck y505f Immunoprecipitation Jurkat-tag cells Lck kinase Lck y192e T cell receptor signalling Lck y192f 
ProteomicsHuman Jurkat T cells Lck-immunoprecipitates LC-MS
Identification of peptides matching to CEP83 in Lck immunoprecipitates from Jurkat T cells by mass spectrometry.
ORGANISM(S): Homo sapiens (Human) 
2026-09-27 | PXD078925 | Pride
Human Lc-ms Lck Jurkat t cells 
ProteomicsThe phosphatase DUSP22/JKAP inhibits UBR2-mediated K63-ubiquitination and activation of Lck in TCR signaling
DUSP22 (also named JKAP) is a dual-specificity phosphatase that inhibits T cell activation. Here we identified the E3 ubiquitin ligase UBR2 as an upstream activator of Lck during T-cell activation. JKAP dephosphorylated UBR2 at two residues, leading to ubiquitin-mediated UBR2 degradation. The SCF (S...
ORGANISM(S): Homo sapiens (Human) 
2024-01-04 | PXD043457 | Pride
Human jurkat t cells 
ProteomicsHuman HEK293T Lck immunoprecipitates
To identify the SUMO E3 ligases specific for tyrosine kinase Lck.
ORGANISM(S): Homo sapiens (Human) 
2026-09-27 | PXD066093 | Pride
Human Lck 
ProteomicsHuman Lck sumoylated site identification
To identify the sumoylation sites of Lck, we performed mass spectrometry analysis by coexpressing Lck with SUMO1-T95R mutant in HEK293T cells.
ORGANISM(S): Homo sapiens (Human) 
2026-09-27 | PXD066090 | Pride
Human Lck Sumoylation 
TranscriptomicsSingle-cell RNA sequencing of spleen-derived LCK-expressing cells from adult zebrafish
Transcriptome data from individual lck:GFP expressing cells isolated from spleens of two adult Zebrafish. LCK is a marker of lymphocytes and here we identified two major subpopulations corresponding to T-cells and NK-like and a minor one of myeloid-like cells. Single cell transcriptomes are matched ...
ORGANISM(S): Danio rerio 
2016-11-01 | E-MTAB-4617 | biostudies-arrayexpress
ProteomicsLck promotes Zap70-dependent LAT phosphorylation by bridging Zap70 to LAT
T cell antigen receptor (TCR) signaling depends upon the kinases Lck and Zap70. Lck phosphorylates the TCR, facilitating Zap70 recruitment to the stimulated TCR. Lck also phosphorylates Zap70, relieving its auto-inhibition and activating its catalytic domain. Zap70 then phosphorylates the critical a...
ORGANISM(S): Homo sapiens (Human) 
2019-11-12 | PXD008258 | Pride
Jurkat t cell line Lat Tcr signaling Mice Lck Zap70 
TranscriptomicsTranscriptional profiling of p56lck-deficient regulatory and memory T cells
Signaling through the T cell antigen receptor is essential for the formation of regulatory T (Treg) cells in the thymus and for their involvement in antigen-directed suppression of immune responses. Using a conditional null allele of the gene encoding p56Lck we show here that T cell antigen recepto...
ORGANISM(S): Mus musculus 
2010-05-22 | E-GEOD-13645 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNA sequencing of lck-GFP transgenic zebrafish
The present study was conducted in the frame of the EU-funded Graphene Flagship project. The aim is to evaluate the impact of graphene oxide (GO) on the (innate) immune system using zebrafish as a model. We previously performed single-cell RNA-sequencing of germ-free zebrafish embryos exposed to GO ...
ORGANISM(S): Danio rerio 
2022-07-23 | E-MTAB-11991 | biostudies-arrayexpress
TranscriptomicsExpression data for Lck-Cre, Med23flox/flox and Med23flox/flox;Lck-Cre thymocytes +/- 3hr exposure to plate bound anti-CD3 antibody
MED23, a subunit of the Mediator coactivator complex, is important for the expression of a subset of MAPK/ERK pathway-dependent target genes; however, the genes in this subset varies between cell types. MAPK/ERK pathway-dependent processes are essential for T-cell development and function, but whet...
ORGANISM(S): Mus musculus 
2014-07-30 | E-GEOD-57061 | biostudies-arrayexpress
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