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HCV proliferation is closely related to three-dimentional cellular condition. In case of blood-borne (bb) HCV culture in HuS-E2 cells, bbHCV was reproduced only from 3D-cultured cells in hollow fibers. Thus, in order to identify novel factors which support HCV proliferation under three-dimentional c...
ORGANISM(S): Homo sapiens 
We optimized a procedure of phosphotyrosine enrichment with immunoprecipitation. Then the optimized procedure was applied into a global analysis of phosphotyrosine status in culture cells treated with Erlotinib.
ORGANISM(S): Homo Sapiens (human) 
proteome, pSTY proteome and pY proteome analysis was performed using 13 gastric cancer cell lines w/wo apatinib treatment.
ORGANISM(S): Homo Sapiens (human) 
Phosphotyrosine status were compared between colorectal cancer cells which is sensitive or resistant to Cetuximab. Activated kinases in resistant cells were examined as targets to overcome the resistance.
ORGANISM(S): Homo Sapiens (human) 
Phosphoproteomics data of Matrigel-embedded HCT116 spheroid, and patient-derived colorecral cancer organoid
ORGANISM(S): Homo Sapiens (human) 
MDCK cell with Src-overexpression (wild type or mutant) were trypsinized, and enriched with Fe-IMAC. After labelling with TMT 10plex reagent, phosphotyrosine peptides were further enriched with phosphotyrosine antibodies. The enriched peptides were analyzed with Q Exactive Plus.
ORGANISM(S): Homo Sapiens (human) 
UMR106 cells were untreated or treated with 5 mM Pi or 100 ng/ml FGF2 for 15 min. The lysates were digested with trypsin, and tyrosine-phosphorylated peptides were enriched by anti-phosphotyrosine antibody and subjected to LC-MS/MS analysis. Raw data were directly analyzed against the SwissProt data...
ORGANISM(S): Rattus Rattus (black Rat) 
UMR106 cells were untreated or treated with 5 mM Pi or 100 ng/ml FGF2 for 15 min. The lysates were digested with trypsin, and tyrosine-phosphorylated peptides were enriched by anti-phosphotyrosine antibody. Several selected peptides including FGFR1 with phosphorylated tyrosine residues were measured...
ORGANISM(S): Rattus Rattus (black Rat) 
Phosphoproteomics were obtained from endoscopic biopsy of gastric cancer.
ORGANISM(S): Homo Sapiens (human) 
Phosphoproteomics were obtained from endoscopic biopsy of gastric cancer.
ORGANISM(S): Homo Sapiens (human) 
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