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TranscriptomicsTimecourse profiling of a C. elegans DGCR8/pash-1 conditional mutant.
Regulation of gene expression by microRNAs (miRNAs) is essential for normal development, but the roles of miRNAs in the physiology of adult animals are poorly understood. We have isolated a conditional allele of DGCR8/pash-1, which allows reversible and rapid inactivation of miRNA synthesis in vivo ...
ORGANISM(S): Caenorhabditis elegans 
2013-10-17 | E-MTAB-1333 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mice hearts comparing microRNA-22 overexpression to wildtype
To explore the role of miR-22 in the heart, we generated miR-22 null and transgenic mice. Cardiac transgenic overexpression of miR-22 in vivo led to cardiac hypertrophy that evolved into progressive dilated cardiomyopathy (DCM) in unstressed hearts. We found that miR-22 directly regulates two transc...
ORGANISM(S): Mus musculus 
2012-12-01 | E-MTAB-501 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mice hearts comparing microRNA-22 KO to wildtype
To explore the role of miR-22 in the heart, we generated miR-22 null and transgenic mice. Absence of miR-22 results in partial embryonic lethality arising from cardiac malformations. miR-22-null mice that survived until adulthood showed normal cardiac structure and function at baseline but were sens...
ORGANISM(S): Mus musculus 
2012-09-11 | E-MTAB-500 | biostudies-arrayexpress
TranscriptomicsDeep sequencing of C. elegans microRNAs under starvation conditions
In nature, animals often face feast or famine conditions. We aimed to identify the miRNAs of Caenorhabditis elegans that changed their expression under starvation conditions in stage L4 larvae. Our results highlight 14 miRNAs that show differential expression in starved versus well-fed larvae. In pa...
ORGANISM(S): Caenorhabditis elegans 
2016-01-27 | E-GEOD-67711 | biostudies-arrayexpress
TranscriptomicsmiR-124 acts through coREST to control the onset of Sema3A sensitivity in navigating retinal growth cones
During axon pathfinding, growth cones commonly exhibit changes in sensitivity to guidance cues that follow a strict timetable, even in the absence of pathway feedback, implicating cell-intrinsic regulation. Cellular timer mechanisms, however, are poorly understood. Here we have investigated microRNA...
ORGANISM(S): Xenopus laevis 
2011-12-12 | E-GEOD-33444 | biostudies-arrayexpress
TranscriptomicsGenome-wide expression analysis of the effects of a parasitic nematode's secreted exosomes on mammalian cells.
We show that a gastrointestinal nematode that infects mice, Heligmosomoides polygyrus, secretes vesicles packaged with specific microRNAs (miRNAs) and Y RNAs as well as a nematode-specific Argonaute protein. The vesicles are of intestinal origin and are enriched for homologs of mammalian proteins fo...
ORGANISM(S): Mus musculus 
2014-12-24 | E-GEOD-55941 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of mouse embryonic stem cell lines with Dgcr8 mutations to investigate the broad role of miRNAs and individual miRNAs through the reintroduction of miRNA mimics
These experiments were conducted as part of a study to derive the targets of miRNAs. Two independent BayGenomics mouse embryonic stem cell lines (XH157 and XG058), each bearing a gene trap between exons 9 and 10 of the Dgcr8 locus (ENSMUST00000115633, Ensembl v53) were further mutagenised by the tar...
ORGANISM(S): Mus musculus 
2012-08-20 | E-MTAB-418 | biostudies-arrayexpress
TranscriptomicsMicroRNA profiling of mouse differentiating embryonic stem cells
To characterise changes in microRNA expression in differentiating mouse ES cells
ORGANISM(S): Mus musculus 
2009-08-15 | E-TABM-621 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse B cells from wild type or mir-155 deficient mice, treated with a-mir155 PNA
B cells were extracted from spleens of mice treated with PNA a-miR155 and vehicle treated mice either wild type or mir-155 deficient. All mice received LPS to induce mir155 expression.
ORGANISM(S): Mus musculus 
2010-03-24 | E-MEXP-2587 | biostudies-arrayexpress
TranscriptomicsmiR-25 regulates Wwp2 and Fbxw7 and promotes reprogramming of mouse somatic cells
To reprogram mouse embryonic fibroblasts (MEFs) to induced Pluripotent Stem Cells (iPSCs), we constructed the PiggyBac (PB) transposon carrying the four Yamanaka factor cDNAs controlled by a CAG promoter (PB-CAG-OCKS, Oct4, cMyc, Klf4 and Sox2). As the baseline reprogramming control, we transfected ...
ORGANISM(S): Mus musculus 
2011-10-10 | E-MTAB-409 | biostudies-arrayexpress
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